Rabbit polyclonal Phospho-Stat3-Y705 antibody (AP0070)

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Reactivity: Human
Applications: WB,IF
Conjugation: Unconjugated
Supplier: ABclonal Inc.

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Rabbit polyclonal Phospho-Stat3-Y705 antibody (AP0070)

Supplier: ABclonal Inc.

Recommended applications: WB,IF

Recommended dilution:

WB 1:500 – 1:2000 IF 1:50 – 1:200

Recommended protocols: check protocols

Image descriptions:

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Alternative names:

Check alternative names for the antibody

Expand

STAT3 antibody, APRF antibody, HIES antibody, FLJ20882 antibody, MGC16063 antibody
1110034C02Rik antibody|Acute Phase Response Factor antibody|Acute-phase response factor antibody|ADMIO antibody|APRF antibody|AW109958 antibody|DNA binding protein APRF antibody|FLJ20882 antibody|HIES antibody|MGC16063 antibody|Signal transducer and activator of transcription 3 (acute phase response factor) antibody|Signal transducer and activator of transcription 3 antibody|STAT 3 antibody|Stat3 antibody|STAT3_HUMAN antibody|Anti-STAT3 antibody [EPR787Y] (ab68153)

SCBT cat No: sc-56747|sc-71792|sc-81523|sc-8059|sc-293059|sc-136193|sc-135649|sc-21876|sc-8001|sc-7993|sc-28296|sc-136194|sc-16317|sc-22160|sc-101804|

Name

Rabbit polyclonal Phospho-Stat3-Y705 antibody

Catalogue No.

AP0070

Reactivity

Human

Immunogen

A phospho specific peptide corresponding to residues surrounding Tyr705 of human Stat3

Host

Rabbit

Applications

WB, IF

Recommended dilution

WB 1:500 – 1:2000
IF 1:50 – 1:200

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

Predicted: 92kDa/Observed: Refer to Figures

Formulation

Phospho-Stat3-Y705 antibody was tube-contained.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Purification

Phospho-Stat3-Y705 antibody was purified using affinity purification.

Storage

Store at -20 Celsius degree. Avoid freeze / thaw cycles.

Alternative antibody names

STAT3 antibody, APRF antibody, HIES antibody, FLJ20882 antibody, MGC16063 antibody

Database links

Human UniProt/Swiss-Prot:P40763

Protein names

STAT3, APRF, HIES, FLJ20882, MGC16063

Protein function

Signal transducer and transcription activator that mediates cellular responses to interleukins, KITLG/SCF, LEP and other growth factors. Once activated, recruits coactivators, such as NCOA1 or MED1, to the promoter region of the target gene . May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4. Binds to the interleukin-6 (IL-6)-responsive elements identified in the promoters of various acute-phase protein genes. Activated by IL31 through IL31RA. Involved in cell cycle regulation by inducing the expression of key genes for the progression from G1 to S phase, such as CCND1 . Mediates the effects of LEP on melanocortin production, body energy homeostasis and lactation (By similarity). May play an apoptotic role by transctivating BIRC5 expression under LEP activation . Cytoplasmic STAT3 represses macroautophagy by inhibiting EIF2AK2/PKR activity.

Protein tissue specificity

Heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.

Protein sequence and domain

Belongs to the transcription factor STAT family.; Contains 1 SH2 domain.

Protein post-translational modifications

Tyrosine phosphorylated upon stimulation with EGF. Tyrosine phosphorylated in response to constitutively activated FGFR1, FGFR2, FGFR3 and FGFR4 (By similarity). Activated through tyrosine phosphorylation by BMX. Tyrosine phosphorylated in response to IL6, IL11, LIF, CNTF, KITLG/SCF, CSF1, EGF, PDGF, IFN-alpha, LEP and OSM. Activated KIT promotes phosphorylation on tyrosine residues and subsequent translocation to the nucleus. Phosphorylated on serine upon DNA damage, probably by ATM or ATR. Serine phosphorylation is important for the formation of stable DNA-binding STAT3 homodimers and maximal transcriptional activity. ARL2BP may participate in keeping the phosphorylated state of STAT3 within the nucleus. Upon LPS challenge, phosphorylated within the nucleus by IRAK1. Upon erythropoietin treatment, phosphorylated on Ser-727 by RPS6KA5. Phosphorylation at Tyr-705 by PTK6 or FER leads to an increase of its transcriptional activity. Dephosphorylation on tyrosine residues by PTPN2 negatively regulates IL6/interleukin-6 signaling.

Protein cellular localization

Cytoplasm. Nucleus. Note: Shuttles between the nucleus and the cytoplasm. Translocated into the nucleus upon tyrosine phosphorylation and dimerization, in response to signaling by activated FGFR1, FGFR2, FGFR3 or FGFR4. Constitutive nuclear presence is independent of tyrosine phosphorylation. Predominantly present in the cytoplasm without stimuli. Upon leukemia inhibitory factor (LIF) stimulation, accumulates in the nucleus. The complex composed of BART and ARL2 plays an important role in the nuclear translocation and retention of STAT3. Identified in a complex with LYN and PAG1.

Background

The Stat3 transcription factor is an important signaling molecule for many cytokines and growth factor receptors (1) and is required for murine fetal development (2). Stat3 is constitutively activated in a number of human tumors (3,4) and possesses oncogenic potential (5) and anti-apoptotic activities (3). Stat3 is activated by phosphorylation at Tyr705, which induces dimerization, nuclear translocation, and DNA binding (6,7). Transcriptional activation seems to be regulated by phosphorylation at Ser727 through the MAPK or mTOR pathways (8,9). Stat3 isoform expression appears to reflect biological function as the relative expression levels of Stat3alpha (86 kDa) and Stat3beta (79 kDa) depend on cell type, ligand exposure, or cell maturation stage (10). It is notable that Stat3beta lacks the serine phosphorylation site within the carboxy-terminal transcriptional activation domain (8).

Research area

All research areas>Transcription Regulators>Stat
(View all antibody categories related to Transcription Regulators)

Note

AntibodyPlus can customize Phospho-Stat3-Y705 antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provides antibody trial sample for your own antibody validation.

Product type

Primary antibody

Supplier

ABclonal Inc.

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