Rabbit polyclonal Phospho-STK11-S334 antibody (AP0601)

$69.00$259.00

Reactivity: Human
Applications: WB
Conjugation: Unconjugated
Supplier: ABclonal Inc.

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Rabbit polyclonal Phospho-STK11-S334 antibody (AP0601)

Supplier: ABclonal Inc.

Recommended applications: WB

Recommended dilution:

WB 1:500 – 1:2000

Recommended protocols: check protocols

Image descriptions:

Click or hover above images to see image description for Rabbit polyclonal Phospho-STK11-S334 antibody.

Alternative names:

Check alternative names for the antibody

Expand

PJS antibody, LKB1 antibody, hLKB1 antibody
hLKB1 antibody|Renal carcinoma antigen NY-REN-19 antibody|Serine/Threonine Kinase 11 antibody|Serine/threonine protein kinase 11 antibody|Serine/threonine protein kinase LKB1 antibody|Serine/threonine protein kinase STK11 antibody|Serine/threonine-protein kinase 11 antibody|Serine/threonine-protein kinase LKB1 antibody|Serine/threonine-protein kinase XEEK1 antibody|Stk11 antibody|STK11_HUMAN antibody|Anti-LKB1 antibody [Ley 37D/G6] (ab15095)

SCBT cat No: sc-5637|sc-5638|

Name

Rabbit polyclonal Phospho-STK11-S334 antibody

Catalogue No.

AP0601

Reactivity

Human

Immunogen

A phospho specific peptide corresponding to residues surrounding S334 of human STK11

Host

Rabbit

Applications

WB

Recommended dilution

WB 1:500 – 1:2000

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

Predicted: 49kDa/Observed: Refer to figures

Formulation

Phospho-STK11-S334 antibody was tube-contained.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Purification

Phospho-STK11-S334 antibody was purified using affinity purification.

Storage

Store at -20 Celsius degree. Avoid freeze / thaw cycles.

Alternative antibody names

PJS antibody, LKB1 antibody, hLKB1 antibody

Database links

Human UniProt/Swiss-Prot:Q15831

Protein names

PJS, LKB1, hLKB1

Protein function

Tumor suppressor serine/threonine-protein kinase that controls the activity of AMP-activated protein kinase (AMPK) family members, thereby playing a role in various processes such as cell metabolism, cell polarity, apoptosis and DNA damage response. Acts by phosphorylating the T-loop of AMPK family proteins, thus promoting their activity: phosphorylates PRKAA1, PRKAA2, BRSK1, BRSK2, MARK1, MARK2, MARK3, MARK4, NUAK1, NUAK2, SIK1, SIK2, SIK3 and SNRK but not MELK. Also phosphorylates non-AMPK family proteins such as STRADA, PTEN and possibly p53/TP53. Acts as a key upstream regulator of AMPK by mediating phosphorylation and activation of AMPK catalytic subunits PRKAA1 and PRKAA2 and thereby regulates processes including: inhibition of signaling pathways that promote cell growth and proliferation when energy levels are low, glucose homeostasis in liver, activation of autophagy when cells undergo nutrient deprivation, and B-cell differentiation in the germinal center in response to DNA damage. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton. Required for cortical neuron polarization by mediating phosphorylation and activation of BRSK1 and BRSK2, leading to axon initiation and specification. Involved in DNA damage response: interacts with p53/TP53 and recruited to the CDKN1A/WAF1 promoter to participate in transcription activation. Able to phosphorylate p53/TP53; the relevance of such result in vivo is however unclear and phosphorylation may be indirect and mediated by downstream STK11/LKB1 kinase NUAK1. Also acts as a mediator of p53/TP53-dependent apoptosis via interaction with p53/TP53: translocates to the mitochondrion during apoptosis and regulates p53/TP53-dependent apoptosis pathways. In vein endothelial cells, inhibits PI3K/Akt signaling activity and thus induces apoptosis in response to the oxidant peroxynitrite (in vitro). Regulates UV radiation-induced DNA damage response mediated by CDKN1A. In association with NUAK1, phosphorylates CDKN1A in response to UV radiation and contributes to its degradation which is necessary for optimal DNA repair .; Isoform 2: Has a role in spermiogenesis.

Protein tissue specificity

Ubiquitously expressed. Strongest expression in testis and fetal liver.

Protein sequence and domain

Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. LKB1 subfamily.; Contains 1 protein kinase domain.

Protein post-translational modifications

Phosphorylated by ATM at Thr-363 following ionizing radiation (IR). Phosphorylation at Ser-428 by RPS6KA1 and/or some PKA is required to inhibit cell growth. Phosphorylation at Ser-428 is also required during neuronal polarization to mediate phosphorylation of BRSK1 and BRSK2 (By similarity). Phosphorylation by PKC/PRKCZ at Ser-428 promotes peroxynitrite-induced nuclear export of STK11, leading to PTEN activation and subsequent inhibition of AKT signaling. Phosphorylation by PKC/PRKCZ at Ser-399 in isoform 2 promotes metformin (or peroxynitrite)-induced nuclear export of STK11 and activation of AMPK. UV radiation-induced phosphorylation at Thr-363 mediates CDKN1A degradation (By similarity).; Acetylated. Deacetylation at Lys-48 enhances cytoplasmic localization and kinase activity in vitro.

Protein cellular localization

Nucleus. Cytoplasm. Membrane . Mitochondrion. Note: A small fraction localizes at membranes (By similarity). Relocates to the cytoplasm when bound to STRAD (STRADA or STRADB) and CAB39/MO25 (CAB39/MO25alpha or CAB39L/MO25beta). Translocates to the mitochondrion during apoptosis. Translocates to the cytoplasm in response to metformin or peroxynitrite treatment. PTEN promotes cytoplasmic localization.; Isoform 2: Nucleus . Cytoplasm . Note: Predominantly nuclear, but translocates to the cytoplasm in response to metformin or peroxynitrite treatment.

Background

This gene, which encodes a member of the serine/threonine kinase family, regulates cell polarity and functions as a tumor suppressor. Mutations in this gene have been associated with Peutz-Jeghers syndrome, an autosomal dominant disorder characterized by the growth of polyps in the gastrointestinal tract, pigmented macules on the skin and mouth, and other neoplasms. Alternate transcriptional splice variants of this gene have been observed but have not been thoroughly characterized.

Research area

All research areas>Kinases and Phosphatases>LKB1
(View all antibody categories related to Kinases and Phosphatases)

Note

AntibodyPlus can customize Phospho-STK11-S334 antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provides antibody trial sample for your own antibody validation.

Product type

Primary antibody

Supplier

ABclonal Inc.

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