Rabbit Polyclonal Phospho-Syk (Y348) antibody (STJ90774)

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Reactivity: Human, Mouse, Rat, Monkey
Applications: WB, IHC, ELISA
Conjugation: Unconjugated
Supplier: St John’s Laboratory Ltd.

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Rabbit Polyclonal Phospho-Syk (Y348) antibody (STJ90774)

Supplier: St John’s Laboratory Ltd.

Recommended applications: WB, IHC, ELISA

Recommended dilution: WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:10000;

Recommended protocols: check protocols

Image descriptions:

Click or hover above images to see image description for Syk (phospho Tyr348) Polyclonal Antibody.

Alternative names:

Check alternative names for the antibody

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SYK antibody,|EC 2.7.10.2 antibody|JAK 3 antibody|JAK L antibody|JAK-3 antibody|Jak3 antibody|JAK3 HUMAN antibody|JAK3_HUMAN antibody|JAKL antibody|Janus kinase 3 (a protein tyrosine kinase, leukocyte) antibody|Janus kinase 3 antibody|Janus Kinase3 antibody|L JAK antibody|L-JAK antibody|Leukocyte janus kinase antibody|LJAK antibody|Protein tyrosine kinase leukocyte antibody|Tyrosine protein kinase JAK3 antibody|Tyrosine-protein kinase JAK3 antibody|Anti-Syk antibody [SYK-01] (ab3993)
SCBT cat No: sc-325227|sc-12913|sc-16852|sc-130606|sc-30037|sc-18205|sc-130219|sc-248320|sc-71794|sc-32275|sc-32276|sc-12413|

 

Name

Syk (phospho Tyr348) Polyclonal Antibody

Catalogue No.

STJ90774

Reactivity

Human, Mouse, Rat, Monkey

Specificity

Phospho-Syk (Y348) Polyclonal Antibody detects endogenous levels of Syk protein only when phosphorylated at Y348.

Immunogen

Synthesized phospho-peptide derived from Syk (phospho Tyr348) at AA range 290-370

Host

Rabbit

Applications

WB, IHC, ELISA

Recommended dilution

WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:10000;

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

72 kDa

Formulation

Syk (phospho Tyr348) Antibody was tube-contained. Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Concentration

1 mg/ml

Purification

Syk (phospho Tyr348) Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage

-20 Celsius degree. Avoid repeated freeze/thaw cycles.

Alternative antibody names

Tyrosine-protein kinase SYK antibody, Spleen tyrosine kinase antibody, p72-Syk antibody

Database links

Human UniProt/Swiss-Prot:P43405;Mouse UniPort/Swiss-Prot: P48025;Rat UniProt/Swiss-Port: Q64725;Human Entrez Gene: 6850;Mouse Entrez Gene: 20963;Rat Entrez Gene: Rn.87407

Protein names

Tyrosine-protein kinase SYK , Spleen tyrosine kinase , p72-Syk

Protein function

Non-receptor tyrosine kinase which mediates signal transduction downstream of a variety of transmembrane receptors including classical immunoreceptors like the B-cell receptor (BCR). Regulates several biological processes including innate and adaptive immunity, cell adhesion, osteoclast maturation, platelet activation and vascular development. Assembles into signaling complexes with activated receptors at the plasma membrane via interaction between its SH2 domains and the receptor tyrosine-phosphorylated ITAM domains. The association with the receptor can also be indirect and mediated by adapter proteins containing ITAM or partial hemITAM domains. The phosphorylation of the ITAM domains is generally mediated by SRC subfamily kinases upon engagement of the receptor. More rarely signal transduction via SYK could be ITAM-independent. Direct downstream effectors phosphorylated by SYK include VAV1, PLCG1, PI-3-kinase, LCP2 and BLNK. Initially identified as essential in B-cell receptor (BCR) signaling, it is necessary for the maturation of B-cells most probably at the pro-B to pre-B transition. Activated upon BCR engagement, it phosphorylates and activates BLNK an adapter linking the activated BCR to downstream signaling adapters and effectors. It also phosphorylates and activates PLCG1 and the PKC signaling pathway. It also phosphorylates BTK and regulates its activity in B-cell antigen receptor (BCR)-coupled signaling. In addition to its function downstream of BCR plays also a role in T-cell receptor signaling. Plays also a crucial role in the innate immune response to fungal, bacterial and viral pathogens. It is for instance activated by the membrane lectin CLEC7A. Upon stimulation by fungal proteins, CLEC7A together with SYK activates immune cells inducing the production of ROS. Also activates the inflammasome and NF-kappa-B-mediated transcription of chemokines and cytokines in presence of pathogens. Regulates neutrophil degranulation and phagocytosis through activation of the MAPK signaling cascade. Also mediates the activation of dendritic cells by cell necrosis stimuli. Also involved in mast cells activation. Also functions downstream of receptors mediating cell adhesion. Relays for instance, integrin-mediated neutrophils and macrophages activation and P-selectin receptor/SELPG-mediated recruitment of leukocytes to inflammatory loci. Plays also a role in non-immune processes. It is for instance involved in vascular development where it may regulate blood and lymphatic vascular separation. It is also required for osteoclast development and function. Functions in the activation of platelets by collagen, mediating PLCG2 phosphorylation and activation. May be coupled to the collagen receptor by the ITAM domain-containing FCER1G. Also activated by the membrane lectin CLEC1B that is required for activation of platelets by PDPN/podoplanin. Involved in platelet adhesion being activated by ITGB3 engaged by fibrinogen. / ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate. / Autoinhibited. Intramolecular binding of the interdomains A and B (also called linker region) to parts of the catalytic domain keep the catalytic center in an inactive conformation. The phosphorylation of the interdomains or the binding of the SH2 domains with dually phosphorylated ITAM domains on transmembrane proteins disrupt those intramolecular interactions allowing the kinase domain to adopt an active conformation. The phosphorylation of SYK and of the ITAM domains which is responsible for SYK activation is essentially mediated by SRC subfamily kinases, like LYN, upon transmembrane receptors engagement. May also be negatively regulated by PTPN6 through dephosphorylation. Downstream signaling adapters and intermediates like BLNK or RHOH may mediate positive and/or negative feedback regulation. Negatively regulated by CBL and CBLB through ubiquitination and probable degradation. Phosphorylates SH3BP2 which in turn may regulate SYK through LYN (By similarity).

Protein tissue specificity

Widely expressed in hematopoietic cells (at protein level). Within the B-cells compartment it is for instance expressed for pro-B-cells to plasma cells.

Protein sequence and domain

The SH2 domains mediate the interaction of SYK with the phosphorylated ITAM domains of transmembrane proteins. Some proteins like CLEC1B have a partial ITAM domain (also called hemITAM) containing a single YxxL motif. The interaction with SYK requires CLEC1B homodimerization. / Belongs to the protein kinase superfamily. Tyr protein kinase family. SYK/ZAP-70 subfamily. / Contains 1 protein kinase domain. / Contains 2 SH2 domains.

Protein post-translational modifications

Ubiquitinated by CBLB after BCR activation; which promotes proteasomal degradation. / Autophosphorylated. Phosphorylated on tyrosine residues by LYN following receptors engagement. Phosphorylation on Tyr-323 creates a binding site for CBL, an adapter protein that serves as a negative regulator of BCR-stimulated calcium ion signaling. Phosphorylation at Tyr-348 creates a binding site for VAV1. Phosphorylation on Tyr-348 and Tyr-352 enhances the phosphorylation and activation of phospholipase C-gamma and the early phase of calcium ion mobilization via a phosphoinositide 3-kinase-independent pathway (By similarity). Phosphorylation on Ser-297 is very common, it peaks 5 minutes after BCR stimulation, and creates a binding site for YWHAG. Phosphorylation at Tyr-630 creates a binding site for BLNK. Dephosphorylated by PTPN6.

Protein cellular localization

Cell membrane / Cytoplasm > cytosol

Research area

All research areas>Kinases and Phosphatases>Syk
(View all antibody categories related to Kinases and Phosphatases)

Note

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Supplier

St John’s Laboratory Ltd.

Product type

Primary antibody

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