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Rabbit Polyclonal Phospho-Tie-2 (Y992) antibody (STJ90597)
Supplier: St John’s Laboratory Ltd.
Recommended applications: WB, ELISA
Recommended dilution: WB 1:500-1:2000; ELISA 1:10000;
Recommended protocols: check protocols
Click or hover above images to see image description for Tie-2 (phospho Tyr992) Polyclonal Antibody.
Check alternative names for the antibodyExpand
TEK antibody, TIE2 antibody, VMCM antibody, VMCM1 antibody,|Angiopoietin 1 receptor antibody|Angiopoietin-1 receptor antibody|CD202b antibody|CD202b antigen antibody|Endothelial tyrosine kinase antibody|Endothelium specific receptor tyrosine kinase 2 antibody|hTIE 2 antibody|hTIE2 antibody|Hyk antibody|p140 TEK antibody|Soluble TIE2 variant 1 antibody|Soluble TIE2 variant 2 antibody|Tek antibody|tek tyrosine kinase antibody|TEK tyrosine kinase endothelial antibody|tek tyrosine kinase, endothelial antibody|TIE 2 antibody|TIE2 antibody|TIE2_HUMAN antibody|Tunica interna endothelial cell kinase antibody|Tyrosine kinase with Ig and EGF homology domains 2 antibody|Tyrosine kinase with Ig and EGF homology domains-2 antibody|Tyrosine protein kinase receptor TEK antibody|Tyrosine protein kinase receptor TIE 2 antibody|Tyrosine-protein kinase receptor TEK antibody|Tyrosine-protein kinase receptor TIE-2 antibody|Venous malformations multiple cutaneous and mucosal antibody|VMCM 1 antibody|VMCM antibody|VMCM1 antibody|Anti-TIE2 antibody [Cl. 16] (ab24859)
SCBT cat No: sc-130607|sc-293322|sc-367459|sc-1394|sc-74443|sc-20716|
Tie-2 (phospho Tyr992) Polyclonal Antibody
|Catalogue No.|| |
Phospho-Tie-2 (Y992) Polyclonal Antibody detects endogenous levels of Tie-2 protein only when phosphorylated at Y992.
Synthesized phospho-peptide derived from Tie-2 (phospho Tyr992) at AA range 930-1010
|Recommended dilution|| |
WB 1:500-1:2000; ELISA 1:10000;
|Molecular weight|| |
Tie-2 (phospho Tyr992) Antibody was tube-contained. Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Tie-2 (phospho Tyr992) Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
-20 Celsius degree. Avoid repeated freeze/thaw cycles.
|Alternative antibody names|| |
Angiopoietin-1 receptor antibody, Endothelial tyrosine kinase antibody, Tunica interna endothelial cell kinase antibody, Tyrosine kinase with Ig and EGF homology domains-2 antibody, Tyrosine-protein kinase receptor TEK antibody, Tyrosine-protein kinase receptor TIE-2 antibody, hTIE2 antibody, p140 TEK antibody, CD antigen CD202b antibody
|Protein names|| |
Angiopoietin-1 receptor , Endothelial tyrosine kinase , Tunica interna endothelial cell kinase , Tyrosine kinase with Ig and EGF homology domains-2 , Tyrosine-protein kinase receptor TEK , Tyrosine-protein kinase receptor TIE-2 , hTIE2 , p140 TEK , CD antigen CD202b
|Protein function|| |
Tyrosine-protein kinase that acts as cell-surface receptor for ANGPT1, ANGPT2 and ANGPT4 and regulates angiogenesis, endothelial cell survival, proliferation, migration, adhesion and cell spreading, reorganization of the actin cytoskeleton, but also maintenance of vascular quiescence. Has anti-inflammatory effects by preventing the leakage of proinflammatory plasma proteins and leukocytes from blood vessels. Required for normal angiogenesis and heart development during embryogenesis. Required for post-natal hematopoiesis. After birth, activates or inhibits angiogenesis, depending on the context. Inhibits angiogenesis and promotes vascular stability in quiescent vessels, where endothelial cells have tight contacts. In quiescent vessels, ANGPT1 oligomers recruit TEK to cell-cell contacts, forming complexes with TEK molecules from adjoining cells, and this leads to preferential activation of phosphatidylinositol 3-kinase and the AKT1 signaling cascades. In migrating endothelial cells that lack cell-cell adhesions, ANGT1 recruits TEK to contacts with the extracellular matrix, leading to the formation of focal adhesion complexes, activation of PTK2/FAK and of the downstream kinases MAPK1/ERK2 and MAPK3/ERK1, and ultimately to the stimulation of sprouting angiogenesis. ANGPT1 signaling triggers receptor dimerization and autophosphorylation at specific tyrosine residues that then serve as binding sites for scaffold proteins and effectors. Signaling is modulated by ANGPT2 that has lower affinity for TEK, can promote TEK autophosphorylation in the absence of ANGPT1, but inhibits ANGPT1-mediated signaling by competing for the same binding site. Signaling is also modulated by formation of heterodimers with TIE1, and by proteolytic processing that gives rise to a soluble TEK extracellular domain. The soluble extracellular domain modulates signaling by functioning as decoy receptor for angiopoietins. TEK phosphorylates DOK2, GRB7, GRB14, PIK3R1; SHC1 and TIE1. / ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate. / Angiopoietin binding leads to receptor dimerization and activation by autophosphorylation at Tyr-992 on the kinase activation loop. Inhibited by staurosporine, K252a, PP2, damnacanthal, SB203580, CEP-11207, CEP-11981 and CE-245677. Inhibited by triazine, thienopyrimidine and thiazolopyrimidine derivatives.
|Protein tissue specificity|| |
Detected in umbilical vein endothelial cells. Proteolytic processing gives rise to a soluble extracellular domain that is detected in blood plasma (at protein level). Predominantly expressed in endothelial cells and their progenitors, the angioblasts. Has been directly found in placenta and lung, with a lower level in umbilical vein endothelial cells, brain and kidney.
|Involvement in disease|| |
Dominantly inherited venous malformations (VMCM) [MIM:600195]: An error of vascular morphogenesis characterized by dilated, serpiginous channels. . Note: The disease is caused by mutations affecting the gene represented in this entry.; Note: May play a role in a range of diseases with a vascular component, including neovascularization of tumors, psoriasis and inflammation.
|Protein sequence and domain|| |
The soluble extracellular domain is functionally active in angiopoietin binding and can modulate the activity of the membrane-bound form by competing for angiopoietins. / Belongs to the protein kinase superfamily. Tyr protein kinase family. Tie subfamily. / Contains 3 EGF-like domains. / Contains 3 fibronectin type-III domains. / Contains 2 Ig-like C2-type (immunoglobulin-like) domains. / Contains 1 protein kinase domain.
|Protein post-translational modifications|| |
Proteolytic processing leads to the shedding of the extracellular domain (soluble TIE-2 alias sTIE-2). / Autophosphorylated on tyrosine residues in response to ligand binding. Autophosphorylation occurs in trans, i.e. one subunit of the dimeric receptor phosphorylates tyrosine residues on the other subunit. Autophosphorylation occurs in a sequential manner, where Tyr-992 in the kinase activation loop is phosphorylated first, followed by autophosphorylation at Tyr-1108 and at additional tyrosine residues. ANGPT1-induced phosphorylation is impaired during hypoxia, due to increased expression of ANGPT2. Phosphorylation is important for interaction with GRB14, PIK3R1 and PTPN11. Phosphorylation at Tyr-1102 is important for interaction with SHC1, GRB2 and GRB7. Phosphorylation at Tyr-1108 is important for interaction with DOK2 and for coupling to downstream signal transduction pathways in endothelial cells. Dephosphorylated by PTPRB. / Ubiquitinated. The phosphorylated receptor is ubiquitinated and internalized, leading to its degradation.
|Protein cellular localization|| |
Cell membrane; Single-pass type I membrane protein / Cell junction / Cell junction > focal adhesion / Cytoplasm > cytoskeleton / Secreted
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St John’s Laboratory Ltd.
|Product type|| |
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