Rabbit polyclonal Phospho-TNK2-Y284 antibody (AP0589)

$69.00$259.00

Reactivity: Human
Applications: WB
Conjugation: Unconjugated
Supplier: ABclonal Inc.

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Rabbit polyclonal Phospho-TNK2-Y284 antibody (AP0589)

Supplier: ABclonal Inc.

Recommended applications: WB

Recommended dilution:

WB 1:500 – 1:2000

Recommended protocols: check protocols

Image descriptions:

Click or hover above images to see image description for Rabbit polyclonal Phospho-TNK2-Y284 antibody.

Alternative names:

Check alternative names for the antibody

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ACK antibody, ACK1 antibody, ACK-1 antibody, p21cdc42Hs antibody
Acetate kinase 1 antibody|Acetokinase 1 antibody|ACK 1 antibody|ACK antibody|ACK-1 antibody|ACK1 antibody|ACK1_HUMAN antibody|Activated Cdc42 associated kinase 1 antibody|Activated CDC42 kinase 1 antibody|Activated p21cdc42Hs kinase antibody|FLJ44758 antibody|FLJ45547 antibody|p21cdc42Hs antibody|TNK 2 antibody|TNK2 antibody|Tyrosine kinase non receptor 2 antibody|Tyrosine kinase non receptor protein 2 antibody|Tyrosine kinase non-receptor protein 2 antibody|Anti-ACK1 antibody (ab135672)

SCBT cat No: sc-28336|sc-323|

Name

Rabbit polyclonal Phospho-TNK2-Y284 antibody

Catalogue No.

AP0589

Reactivity

Human

Immunogen

A phospho specific peptide corresponding to residues surrounding Y284 of human TNK2

Host

Rabbit

Applications

WB

Recommended dilution

WB 1:500 – 1:2000

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

Predicted: 115kDa/Observed: Refer to figures

Formulation

Phospho-TNK2-Y284 antibody was tube-contained.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Purification

Phospho-TNK2-Y284 antibody was purified using affinity purification.

Storage

Store at -20 Celsius degree. Avoid freeze / thaw cycles.

Alternative antibody names

ACK antibody, ACK1 antibody, ACK-1 antibody, p21cdc42Hs antibody

Database links

Human UniProt/Swiss-Prot:Q07912

Protein names

ACK, ACK1, ACK-1, p21cdc42Hs

Protein function

Non-receptor tyrosine-protein and serine/threonine-protein kinase that is implicated in cell spreading and migration, cell survival, cell growth and proliferation. Transduces extracellular signals to cytosolic and nuclear effectors. Phosphorylates AKT1, AR, MCF2, WASL and WWOX. Implicated in trafficking and clathrin-mediated endocytosis through binding to epidermal growth factor receptor (EGFR) and clathrin. Binds to both poly- and mono-ubiquitin and regulates ligand-induced degradation of EGFR, thereby contributing to the accumulation of EGFR at the limiting membrane of early endosomes. Downstream effector of CDC42 which mediates CDC42-dependent cell migration via phosphorylation of BCAR1. May be involved both in adult synaptic function and plasticity and in brain development. Activates AKT1 by phosphorylating it on ‘Tyr-176’. Phosphorylates AR on ‘Tyr-267’ and ‘Tyr-363’ thereby promoting its recruitment to androgen-responsive enhancers (AREs). Phosphorylates WWOX on ‘Tyr-287’. Phosphorylates MCF2, thereby enhancing its activity as a guanine nucleotide exchange factor (GEF) toward Rho family proteins. Contributes to the control of AXL receptor levels. Confers metastatic properties on cancer cells and promotes tumor growth by negatively regulating tumor suppressor such as WWOX and positively regulating pro-survival factors such as AKT1 and AR.

Protein tissue specificity

The Tyr-284 phosphorylated form shows a significant increase in expression in breast cancers during the progressive stages i.e. normal to hyperplasia (ADH), ductal carcinoma in situ (DCIS), invasive ductal carcinoma (IDC) and lymph node metastatic (LNMM) stages. It also shows a significant increase in expression in prostate cancers during the progressive stages.

Protein sequence and domain

The EBD (EGFR-binding domain) domain is necessary for interaction with EGFR.; The SAM-like domain is necessary for NEDD4-mediated ubiquitination. Promotes membrane localization and dimerization to allow for autophosphorylation.; The UBA domain binds both poly- and mono-ubiquitin.Belongs to the protein kinase superfamily. Tyr protein kinase family.; Contains 1 CRIB domain.; Contains 1 protein kinase domain.; Contains 1 SH3 domain.; Contains 1 UBA domain.

Protein post-translational modifications

Autophosphorylation regulates kinase activity. Phosphorylation on Tyr-518 is required for interaction with SRC and is observed during association with clathrin-coated pits.; Polyubiquitinated by NEDD4 and NEDD4L. Degradation can be induced by EGF and is lysosome-dependent (By similarity).

Protein cellular localization

Cell membrane. Nucleus. Endosome. Cell junction, adherens junction . Cytoplasmic vesicle membrane; Peripheral membrane protein; Cytoplasmic side. Cytoplasmic vesicle, clathrin-coated vesicle. Membrane, clathrin-coated pit. Note: The Tyr-284 phosphorylated form is found both in the membrane and nucleus. Colocalizes with EGFR on endosomes. Nuclear translocation is CDC42-dependent.

Background

This gene encodes a tyrosine kinase that binds Cdc42Hs in its GTP-bound form and inhibits both the intrinsic and GTPase-activating protein (GAP)-stimulated GTPase activity of Cdc42Hs. This binding is mediated by a unique sequence of 47 amino acids C-terminal to an SH3 domain. The protein may be involved in a regulatory mechanism that sustains the GTP-bound active form of Cdc42Hs and which is directly linked to a tyrosine phosphorylation signal transduction pathway. Several alternatively spliced transcript variants have been identified from this gene, but the full-length nature of only two transcript variants has been determined.

Research area

All research areas>Kinases and Phosphatases>ACK
(View all antibody categories related to Kinases and Phosphatases)

Note

AntibodyPlus can customize Phospho-TNK2-Y284 antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provides antibody trial sample for your own antibody validation.

Product type

Primary antibody

Supplier

ABclonal Inc.

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