Rabbit Polyclonal Phospho-Trk A (Y701) antibody (STJ90937)

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Reactivity: Human, Mouse, Rat
Applications: WB, IHC, ELISA
Conjugation: Unconjugated
Supplier: St John’s Laboratory Ltd.

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Rabbit Polyclonal Phospho-Trk A (Y701) antibody (STJ90937)

Supplier: St John’s Laboratory Ltd.

Recommended applications: WB, IHC, ELISA

Recommended dilution: WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:40000;

Recommended protocols: check protocols

Image descriptions:

Click or hover above images to see image description for Trk A (phospho Tyr701) Polyclonal Antibody.

Alternative names:

Check alternative names for the antibody

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NTRK1 antibody, MTC antibody, TRK antibody, TRKA antibody,|MTC antibody|Neurotrophic tyrosine kinase receptor type 1 antibody|Neurotrophin receptor tyrosine kinase type 2 antibody|NTRK1 antibody|NTRK2 antibody|TRK antibody|TRKA antibody|TRKB antibody|Tyrosine kinase receptor A antibody|Tyrosine kinase receptor B antibody|Anti-TRKA+B antibody [1.A.37] (ab18137)
SCBT cat No: sc-135645|sc-66159|sc-135790|sc-135789|sc-71947|sc-5839|sc-398471|

 

Name

Trk A (phospho Tyr701) Polyclonal Antibody

Catalogue No.

STJ90937

Reactivity

Human, Mouse, Rat

Specificity

Phospho-Trk A (Y701) Polyclonal Antibody detects endogenous levels of Trk A protein only when phosphorylated at Y701.

Immunogen

Synthesized phospho-peptide derived from Trk A (phospho Tyr701) at AA range 640-720

Host

Rabbit

Applications

WB, IHC, ELISA

Recommended dilution

WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:40000;

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

87 kDa

Formulation

Trk A (phospho Tyr701) Antibody was tube-contained. Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Concentration

1 mg/ml

Purification

Trk A (phospho Tyr701) Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage

-20 Celsius degree. Avoid repeated freeze/thaw cycles.

Alternative antibody names

High affinity nerve growth factor receptor antibody, Neurotrophic tyrosine kinase receptor type 1 antibody, TRK1-transforming tyrosine kinase protein antibody, Tropomyosin-related kinase A antibody, Tyrosine kinase receptor antibody, Tyrosine kinase receptor A antibody, Trk-A antibody, gp140trk antibody, p140-TrkA antibody

Database links

Human UniProt/Swiss-Prot:P04629;Mouse UniPort/Swiss-Prot: Q3UFB7;Rat UniProt/Swiss-Port: P35739;Human Entrez Gene: 4914;Mouse Entrez Gene: 18211;Rat Entrez Gene: Rn.39098

Protein names

High affinity nerve growth factor receptor , Neurotrophic tyrosine kinase receptor type 1 , TRK1-transforming tyrosine kinase protein , Tropomyosin-related kinase A , Tyrosine kinase receptor , Tyrosine kinase receptor A , Trk-A , gp140trk , p140-TrkA

Protein function

Receptor tyrosine kinase involved in the development and the maturation of the central and peripheral nervous systems through regulation of proliferation, differentiation and survival of sympathetic and nervous neurons. High affinity receptor for NGF which is its primary ligand, it can also bind and be activated by NTF3/neurotrophin-3. However, NTF3 only supports axonal extension through NTRK1 but has no effect on neuron survival. Upon dimeric NGF ligand-binding, undergoes homodimerization, autophosphorylation and activation. Recruits, phosphorylates and/or activates several downstream effectors including SHC1, FRS2, SH2B1, SH2B2 and PLCG1 that regulate distinct overlapping signaling cascades driving cell survival and differentiation. Through SHC1 and FRS2 activates a GRB2-Ras-MAPK cascade that regulates cell differentiation and survival. Through PLCG1 controls NF-Kappa-B activation and the transcription of genes involved in cell survival. Through SHC1 and SH2B1 controls a Ras-PI3 kinase-AKT1 signaling cascade that is also regulating survival. In absence of ligand and activation, may promote cell death, making the survival of neurons dependent on trophic factors. / Isoform TrkA-III is resistant to NGF, constitutively activates AKT1 and NF-kappa-B and is unable to activate the Ras-MAPK signaling cascade. Antagonizes the anti-proliferative NGF-NTRK1 signaling that promotes neuronal precursors differentiation. Isoform TrkA-III promotes angiogenesis and has oncogenic activity when overexpressed. / ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate. / The pro-survival signaling effect of NTRK1 in neurons requires its endocytosis into signaling early endosomes and its retrograde axonal transport. This is regulated by different proteins including CFL1, RAC1 and SORT1. NTF3 is unable to induce this signaling probably due to the lability of the NTF3-NTRK1 complex in endosomes. SH2D1A inhibits the autophosphorylation of the receptor, and alters the recruitment and activation of downstream effectors and signaling cascades (By similarity). Regulated by NGFR (By similarity).

Protein tissue specificity

Isoform TrkA-I is found in most non-neuronal tissues. Isoform TrkA-II is primarily expressed in neuronal cells. TrkA-III is specifically expressed by pluripotent neural stem and neural crest progenitors.

Involvement in disease

Congenital insensitivity to pain with anhidrosis (CIPA) [MIM:256800]: Characterized by a congenital insensitivity to pain, anhidrosis (absence of sweating), absence of reaction to noxious stimuli, self-mutilating behavior, and mental retardation. This rare autosomal recessive disorder is also known as congenital sensory neuropathy with anhidrosis or hereditary sensory and autonomic neuropathy type IV or familial dysautonomia type II. . Note: The disease is caused by mutations affecting the gene represented in this entry.; Note: Chromosomal aberrations involving NTRK1 are found in papillary thyroid carcinomas (PTCs) (PubMed:2869410, PubMed:7565764, PubMed:1532241). Translocation t(1;3)(q21;q11) with TFG generates the TRKT3 (TRK-T3) transcript by fusing TFG to the 3′-end of NTRK1 (PubMed:7565764). A rearrangement with TPM3 generates the TRK transcript by fusing TPM3 to the 3′-end of NTRK1 (PubMed:2869410). An intrachromosomal rearrangement that links the protein kinase domain of NTRK1 to the 5′-end of the TPR gene forms the fusion protein TRK-T1. TRK-T1 is a 55 kDa protein reacting with antibodies against the C-terminus of the NTRK1 protein (PubMed:1532241). .

Protein sequence and domain

The transmembrane domain mediates interaction with KIDINS220. / The extracellular domain mediates interaction with NGFR. / Belongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily. / Contains 2 Ig-like C2-type (immunoglobulin-like) domains. / Contains 2 LRR (leucine-rich) repeats. / Contains 1 LRRCT domain. / Contains 1 protein kinase domain.

Protein post-translational modifications

Ligand-mediated autophosphorylation. Interaction with SQSTM1 is phosphotyrosine-dependent. Autophosphorylation at Tyr-496 mediates interaction and phosphorylation of SHC1. / N-glycosylated (Probable). Isoform TrkA-I is N-glycosylated. / Ubiquitinated. Undergoes polyubiquitination upon activation; regulated by NGFR. Ubiquitination regulates the internalization of the receptor (By similarity).

Protein cellular localization

Cell membrane / Single-pass type I membrane protein / Early endosome membrane / Late endosome membrane

Research area

All research areas>Neurobiology>Trk
(View all antibody categories related to Neurobiology)

Note

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Supplier

St John’s Laboratory Ltd.

Product type

Primary antibody

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