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Rabbit Polyclonal Phospho-Trk B (Y516) antibody (STJ90429)
Supplier: St John’s Laboratory Ltd.
Recommended applications: WB, IHC, ELISA
Recommended dilution: WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:5000;
Recommended protocols: check protocols
Click or hover above images to see image description for Trk B (phospho Tyr516) Polyclonal Antibody.
Check alternative names for the antibodyExpand
NTRK2 antibody, TRKB antibody,|AI848316 antibody|tyrosine kinase receptor B antibody|Tyrosine receptor kinase B antibody|Anti-TrkB antibody (ab18987)
SCBT cat No: sc-135645|sc-66159|sc-135790|sc-135789|sc-71947|sc-5839|sc-398471|
Trk B (phospho Tyr516) Polyclonal Antibody
|Catalogue No.|| |
Human, Mouse, Rat
Phospho-Trk B (Y516) Polyclonal Antibody detects endogenous levels of Trk B protein only when phosphorylated at Y516.
Synthesized phospho-peptide derived from Trk B (phospho Tyr516) at AA range 450-530
WB, IHC, ELISA
|Recommended dilution|| |
WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:5000;
|Molecular weight|| |
Trk B (phospho Tyr516) Antibody was tube-contained. Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Trk B (phospho Tyr516) Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
-20 Celsius degree. Avoid repeated freeze/thaw cycles.
|Alternative antibody names|| |
BDNF/NT-3 growth factors receptor antibody, GP145-TrkB antibody, Trk-B antibody, Neurotrophic tyrosine kinase receptor type 2 antibody, TrkB tyrosine kinase antibody, Tropomyosin-related kinase B antibody
|Protein names|| |
BDNF/NT-3 growth factors receptor , GP145-TrkB , Trk-B , Neurotrophic tyrosine kinase receptor type 2 , TrkB tyrosine kinase , Tropomyosin-related kinase B
|Protein function|| |
Receptor tyrosine kinase involved in the development and the maturation of the central and the peripheral nervous systems through regulation of neuron survival, proliferation, migration, differentiation, and synapse formation and plasticity. Receptor for BDNF/brain-derived neurotrophic factor and NTF4/neurotrophin-4. Alternatively can also bind NTF3/neurotrophin-3 which is less efficient in activating the receptor but regulates neuron survival through NTRK2. Upon ligand-binding, undergoes homodimerization, autophosphorylation and activation. Recruits, phosphorylates and/or activates several downstream effectors including SHC1, FRS2, SH2B1, SH2B2 and PLCG1 that regulate distinct overlapping signaling cascades. Through SHC1, FRS2, SH2B1, SH2B2 activates the GRB2-Ras-MAPK cascade that regulates for instance neuronal differentiation including neurite outgrowth. Through the same effectors controls the Ras-PI3 kinase-AKT1 signaling cascade that mainly regulates growth and survival. Through PLCG1 and the downstream protein kinase C-regulated pathways controls synaptic plasticity. Thereby, plays a role in learning and memory by regulating both short term synaptic function and long-term potentiation. PLCG1 also leads to NF-Kappa-B activation and the transcription of genes involved in cell survival. Hence, it is able to suppress anoikis, the apoptosis resulting from loss of cell-matrix interactions. May also play a role in neutrophin-dependent calcium signaling in glial cells and mediate communication between neurons and glia. / ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate. / The neuronal activity and the influx of calcium positively regulate the kinase activity and the internalization of the receptor which are both important for active signaling. Regulated by NGFR that may control the internalization of the receptor. NGFR may also stimulate the activation by BDNF compared to NTF3 and NTF4. SH2D1A inhibits the autophosphorylation of the receptor, and alters the recruitment and activation of downstream effectors and signaling cascades. The formation of active receptors dimers able to fully transduce the ligand-mediated signal, may be negatively regulated by the formation of inactive heterodimers with the non-catalytic isoforms (By similarity).
|Protein tissue specificity|| |
Isoform TrkB is expressed in the central and peripheral nervous system. In the central nervous system (CNS), expression is observed in the cerebral cortex, hippocampus, thalamus, choroid plexus, granular layer of the cerebellum, brain stem, and spinal cord. In the peripheral nervous system, it is expressed in many cranial ganglia, the ophthalmic nerve, the vestibular system, multiple facial structures, the submaxillary glands, and dorsal root ganglia. Isoform TrkB-T1 is mainly expressed in the brain but also detected in other tissues including pancreas, kidney and heart. Isoform TrkB-T-Shc is predominantly expressed in the brain.
|Involvement in disease|| |
Obesity, hyperphagia, and developmental delay (OBHD) [MIM:613886]: A disorder characterized by early-onset obesity, hyperphagia, and severe developmental delay in motor function, speech, and language. . Note: The disease is caused by mutations affecting the gene represented in this entry.
|Protein sequence and domain|| |
Belongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily. / Contains 2 Ig-like C2-type (immunoglobulin-like) domains. / Contains 2 LRR (leucine-rich) repeats. / Contains 1 LRRCT domain. / Contains 1 LRRNT domain. / Contains 1 protein kinase domain.
|Protein post-translational modifications|| |
Phosphorylated. Undergoes ligand-mediated autophosphorylation that is required for interaction with SHC1 and PLCG1 and other downstream effectors. Isoform TrkB-T-Shc is not phosphorylated. / Ubiquitinated. Undergoes polyubiquitination upon activation; regulated by NGFR. Ubiquitination regulates the internalization of the receptor (By similarity).
|Protein cellular localization|| |
Cell membrane; Single-pass type I membrane protein / Endosome membrane
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St John’s Laboratory Ltd.
|Product type|| |
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