Rabbit Polyclonal Phospho-VASP (S238) antibody (STJ90432)

$99.00$319.00

Reactivity: Human, Mouse, Rat
Applications: WB, IHC, ELISA
Conjugation: Unconjugated
Supplier: St John’s Laboratory Ltd.

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Rabbit Polyclonal Phospho-VASP (S238) antibody (STJ90432)

Supplier: St John’s Laboratory Ltd.

Recommended applications: WB, IHC, ELISA

Recommended dilution: WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:20000;

Recommended protocols: check protocols

Image descriptions:

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Alternative names:

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VASP antibody,||
SCBT cat No: To be updated

 

Name

VASP (phospho Ser238) Polyclonal Antibody

Catalogue No.

STJ90432

Reactivity

Human, Mouse, Rat

Specificity

Phospho-VASP (S238) Polyclonal Antibody detects endogenous levels of VASP protein only when phosphorylated at S238.

Immunogen

Synthesized phospho-peptide derived from VASP (phospho Ser238) at AA range 180-260

Host

Rabbit

Applications

WB, IHC, ELISA

Recommended dilution

WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:20000;

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

46/50 kDa

Formulation

VASP (phospho Ser238) Antibody was tube-contained. Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Concentration

1 mg/ml

Purification

VASP (phospho Ser238) Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage

-20 Celsius degree. Avoid repeated freeze/thaw cycles.

Alternative antibody names

Vasodilator-stimulated phosphoprotein antibody, VASP antibody

Database links

Human UniProt/Swiss-Prot:P50552;Mouse UniPort/Swiss-Prot: P70460;Rat UniProt/Swiss-Port: B5DEX4;Human Entrez Gene: 7408;Mouse Entrez Gene: 22323;Rat Entrez Gene: Rn.98750

Protein names

Vasodilator-stimulated phosphoprotein , VASP

Protein function

Ena/VASP proteins are actin-associated proteins involved in a range of processes dependent on cytoskeleton remodeling and cell polarity such as axon guidance, lamellipodial and filopodial dynamics, platelet activation and cell migration. VASP promotes actin filament elongation. It protects the barbed end of growing actin filaments against capping and increases the rate of actin polymerization in the presence of capping protein. VASP stimulates actin filament elongation by promoting the transfer of profilin-bound actin monomers onto the barbed end of growing actin filaments. Plays a role in actin-based mobility of Listeria monocytogenes in host cells. Regulates actin dynamics in platelets and plays an important role in regulating platelet aggregation.

Protein tissue specificity

Highly expressed in platelets.

Protein sequence and domain

The EVH2 domain is comprised of 3 regions. Block A is a thymosin-like domain required for G-actin binding. The KLKR motif within this block is essential for the G-actin binding and for actin polymerization. Block B is required for F-actin binding and subcellular location, and Block C for tetramerization. / The WH1 domain mediates interaction with XIRP1. / Belongs to the Ena/VASP family. / Contains 1 WH1 domain.

Protein post-translational modifications

Major substrate for cAMP-dependent (PKA) and cGMP-dependent protein kinase (PKG) in platelets. The preferred site for PKA is Ser-157, the preferred site for PKG/PRKG1, Ser-239. In ADP-activated platelets, phosphorylation by PKA or PKG on Ser-157 leads to fibrinogen receptor inhibition. Phosphorylation on Thr-278 requires prior phosphorylation on Ser-157 and Ser-239. In response to phorbol ester (PMA) stimulation, phosphorylated by PKC/PRKCA. In response to thrombin, phosphorylated by both PKC and ROCK1. Phosphorylation at Thr-278 by AMPK does not require prior phosphorylation at Ser-157 or Ser-239. Phosphorylation at Ser-157 by PKA is required for localization to the tight junctions in epithelial cells. Phosphorylation modulates F-actin binding, actin filament elongation and platelet activation. Phosphorylation at Ser-322 by AMPK also alters actin filament binding. Carbon monoxide (CO) promotes phosphorylation at Ser-157, while nitric oxide (NO) promotes phosphorylation at Ser-157, but also at Ser-239. Response to NO and CO is blunted in platelets from diabetic patients, and VASP is not phosphorylated efficiently at Ser-157 and Ser-239.

Protein cellular localization

Cytoplasm / Cytoplasm > cytoskeleton / Cell junction > focal adhesion / Cell junction > tight junction / Cell projection > lamellipodium membrane / Cell projection > filopodium membrane

Research area

All research areas>Structural Proteins>VASP
(View all antibody categories related to Structural Proteins)

Note

AntibodyPlus can customize VASP (phospho Ser238) Antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provide antibody trial sample for your own antibody validation and collects antibody reviews.

Supplier

St John’s Laboratory Ltd.

Product type

Primary antibody

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