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Rabbit Polyclonal Phospho-Wee1 (S642) antibody (STJ90550)
Supplier: St John’s Laboratory Ltd.
Recommended applications: WB, ELISA
Recommended dilution: WB 1:500-1:2000; ELISA 1:5000;
Recommended protocols: check protocols
Click or hover above images to see image description for Wee1 (phospho Ser642) Polyclonal Antibody.
Check alternative names for the antibodyExpand
WEE1 antibody,|DKFZp686I18166 antibody|EC 126.96.36.199 antibody|FLJ16446 antibody|MGC105683 antibody|OTTHUMP00000231338 antibody|OTTHUMP00000231339 antibody|Wee 1 antibody|WEE 1 homolog 1 (S. pombe) antibody|WEE1 antibody|WEE1 homolog (S. pombe) antibody|Wee1 homolog antibody|WEE1 homolog S. pombe antibody|Wee1 like protein kinase antibody|Wee1 tyrosine kinase antibody|Wee1+ homolog antibody|Wee1+ S. pombe homolog antibody|WEE1, S. pombe, homolog of antibody|WEE1, somatic antibody|Wee1-like protein kinase antibody|WEE1_HUMAN antibody|WEE1A antibody|Wee1A kinase antibody|WEE1hu antibody|Anti-Wee1 antibody (ab2315)
SCBT cat No: To be updated
Wee1 (phospho Ser642) Polyclonal Antibody
|Catalogue No.|| |
Human, Mouse, Rat
Phospho-Wee1 (S642) Polyclonal Antibody detects endogenous levels of Wee1 protein only when phosphorylated at S642.
Synthesized phospho-peptide derived from Wee1 (phospho Ser642) at AA range 580-660
|Recommended dilution|| |
WB 1:500-1:2000; ELISA 1:5000;
|Molecular weight|| |
Wee1 (phospho Ser642) Antibody was tube-contained. Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Wee1 (phospho Ser642) Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
-20 Celsius degree. Avoid repeated freeze/thaw cycles.
|Alternative antibody names|| |
Wee1-like protein kinase antibody, WEE1hu antibody, Wee1A kinase antibody
|Protein names|| |
Wee1-like protein kinase , WEE1hu , Wee1A kinase
|Protein function|| |
Acts as a negative regulator of entry into mitosis (G2 to M transition) by protecting the nucleus from cytoplasmically activated cyclin B1-complexed CDK1 before the onset of mitosis by mediating phosphorylation of CDK1 on ‘Tyr-15’. Specifically phosphorylates and inactivates cyclin B1-complexed CDK1 reaching a maximum during G2 phase and a minimum as cells enter M phase. Phosphorylation of cyclin B1-CDK1 occurs exclusively on ‘Tyr-15’ and phosphorylation of monomeric CDK1 does not occur. Its activity increases during S and G2 phases and decreases at M phase when it is hyperphosphorylated. A correlated decrease in protein level occurs at M/G1 phase, probably due to its degradation. / ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate. / Mg2+ / Synthesis is increased during S and G2 phases, presumably by an increase in transcription; activity is decreased by phosphorylation during m phase. Protein levels fall in M phase as a result of decreased synthesis combined with degradation. Activity seems to be negatively regulated by phosphorylation upon entry into mitosis, although N-terminal phosphorylation might also regulate the protein stability via protection from proteolysis or might regulate the subcellular location.
|Protein sequence and domain|| |
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. WEE1 subfamily. / Contains 1 protein kinase domain.
|Protein post-translational modifications|| |
Phosphorylated during M and G1 phases. Also autophosphorylated. Phosphorylation at Ser-642 by BRSK1 and BRSK2 in post-mitotic neurons, leads to down-regulate WEE1 activity in polarized neurons. Phosphorylated at Ser-53 and Ser-123 by PLK1 and CDK1, respectively, generating an signal for degradation that can be recognized by the SCF(BTRC) complex, leading to its ubiquitination and degradation at the onset of G2/M phase. / Dephosphorylated at Thr-239 by CTDP1. / Ubiquitinated and degraded at the onset of G2/M phase.
|Protein cellular localization|| |
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St John’s Laboratory Ltd.
|Product type|| |
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