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Rabbit Polyclonal Phospho-WNK1 (T60) antibody (STJ90776)
Supplier: St John’s Laboratory Ltd.
Recommended applications: WB, IHC, ELISA
Recommended dilution: WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:5000;
Recommended protocols: check protocols
Click or hover above images to see image description for WNK1 (phospho Thr60) Polyclonal Antibody.
Check alternative names for the antibodyExpand
WNK1 antibody, HSN2 antibody, KDP antibody, KIAA0344 antibody, PRKWNK1 antibody,|Erythrocyte 65 kDa protein antibody|HSAN2 antibody|HSN2 antibody|hWNK1 antibody|KDP antibody|KIAA0344 antibody|Kinase deficient protein antibody|MGC163339 antibody|MGC163341 antibody|p65 antibody|PRKWNK1 antibody|Prostate derived sterile 20 like kinase antibody|Protein kinase lysine deficient 1 antibody|Protein kinase lysine-deficient 1 antibody|Protein kinase with no lysine 1 antibody|PSK antibody|Serine/threonine protein kinase WNK1 antibody|Serine/threonine-protein kinase WNK1 antibody|With no K antibody|WNK lysine deficient protein kinase 1 antibody|WNK1 antibody|WNK1_HUMAN antibody|Anti-WNK1 antibody (ab53151)
SCBT cat No: To be updated
WNK1 (phospho Thr60) Polyclonal Antibody
|Catalogue No.|| |
Human, Mouse, Rat
Phospho-WNK1 (T60) Polyclonal Antibody detects endogenous levels of WNK1 protein only when phosphorylated at T60.
Synthesized phospho-peptide derived from WNK1 (phospho Thr60) at AA range 40-120
WB, IHC, ELISA
|Recommended dilution|| |
WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:5000;
|Molecular weight|| |
WNK1 (phospho Thr60) Antibody was tube-contained. Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
WNK1 (phospho Thr60) Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
-20 Celsius degree. Avoid repeated freeze/thaw cycles.
|Alternative antibody names|| |
Serine/threonine-protein kinase WNK1 antibody, Erythrocyte 65 kDa protein antibody, p65 antibody, Kinase deficient protein antibody, Protein kinase lysine-deficient 1 antibody, Protein kinase with no lysine 1 antibody, hWNK1 antibody
|Protein names|| |
Serine/threonine-protein kinase WNK1 , Erythrocyte 65 kDa protein , p65 , Kinase deficient protein , Protein kinase lysine-deficient 1 , Protein kinase with no lysine 1 , hWNK1
|Protein function|| |
Serine/threonine kinase which plays an important role in the regulation of electrolyte homeostasis, cell signaling, survival, and proliferation. Acts as an activator and inhibitor of sodium-coupled chloride cotransporters and potassium-coupled chloride cotransporters respectively. Activates SCNN1A, SCNN1B, SCNN1D and SGK1. Controls sodium and chloride ion transport by inhibiting the activity of WNK4, by either phosphorylating the kinase or via an interaction between WNK4 and the autoinhibitory domain of WNK1. WNK4 regulates the activity of the thiazide-sensitive Na-Cl cotransporter, SLC12A3, by phosphorylation. WNK1 may also play a role in actin cytoskeletal reorganization. Phosphorylates NEDD4L. Acts as a scaffold to inhibit SLC4A4, SLC26A6 as well as CFTR activities and surface expression, recruits STK39 which mediates the inhibition (By similarity). / ATP + a protein = ADP + a phosphoprotein. / Mg2+ / By hypertonicity. Activation requires autophosphorylation of Ser-382. Phosphorylation of Ser-378 also promotes increased activity (By similarity).
|Protein tissue specificity|| |
Widely expressed, with highest levels observed in the testis, heart, kidney and skeletal muscle. Isoform 3 is kidney-specific and specifically expressed in the distal convoluted tubule (DCT) and connecting tubule (CNT) of the nephron.
|Involvement in disease|| |
Pseudohypoaldosteronism 2C (PHA2C) [MIM:614492]: An autosomal dominant disorder characterized by severe hypertension, hyperkalemia, hyperchloremia, mild hyperchloremic metabolic acidosis in some cases, and correction of physiologic abnormalities by thiazide diuretics. . Note: The disease is caused by mutations affecting the gene represented in this entry.; Neuropathy, hereditary sensory and autonomic, 2A (HSAN2A) [MIM:201300]: A form of hereditary sensory and autonomic neuropathy, a genetically and clinically heterogeneous group of disorders characterized by degeneration of dorsal root and autonomic ganglion cells, and by sensory and/or autonomic abnormalities. HSAN2A is an autosomal recessive disorder characterized by impairment of pain, temperature and touch sensation, onset of symptoms in infancy or early childhood, occurrence of distal extremity pathologies (paronychia, whitlows, ulcers, and Charcot joints), frequent amputations, sensory loss that affects all modalities of sensation (lower and upper limbs and perhaps the trunk as well), absence or diminution of tendon reflexes (usually in all limbs), minimal autonomic dysfunction, absence of sensory nerve action potentials, and virtual absence of myelinated fibers with decreased numbers of unmyelinated fibers in sural nerves. . Note: The disease is caused by mutations affecting the gene represented in this entry.
|Protein sequence and domain|| |
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. WNK subfamily. / Contains 1 protein kinase domain.
|Protein post-translational modifications|| |
O-glycosylated. / Ubiquitinated in vitro by the BCR(KLHL3) complex and in vivo by a BCR(KLHL2) complex, leading to proteasomal degradation.
|Protein cellular localization|| |
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St John’s Laboratory Ltd.
|Product type|| |
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