Rabbit Polyclonal Phospho-WNK1 (T60) antibody (STJ90776)

$99.00$319.00

Reactivity: Human, Mouse, Rat
Applications: WB, IHC, ELISA
Conjugation: Unconjugated
Supplier: St John’s Laboratory Ltd.

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Rabbit Polyclonal Phospho-WNK1 (T60) antibody (STJ90776)

Supplier: St John’s Laboratory Ltd.

Recommended applications: WB, IHC, ELISA

Recommended dilution: WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:5000;

Recommended protocols: check protocols

Image descriptions:

Click or hover above images to see image description for WNK1 (phospho Thr60) Polyclonal Antibody.

Alternative names:

Check alternative names for the antibody

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WNK1 antibody, HSN2 antibody, KDP antibody, KIAA0344 antibody, PRKWNK1 antibody,|Erythrocyte 65 kDa protein antibody|HSAN2 antibody|HSN2 antibody|hWNK1 antibody|KDP antibody|KIAA0344 antibody|Kinase deficient protein antibody|MGC163339 antibody|MGC163341 antibody|p65 antibody|PRKWNK1 antibody|Prostate derived sterile 20 like kinase antibody|Protein kinase lysine deficient 1 antibody|Protein kinase lysine-deficient 1 antibody|Protein kinase with no lysine 1 antibody|PSK antibody|Serine/threonine protein kinase WNK1 antibody|Serine/threonine-protein kinase WNK1 antibody|With no K antibody|WNK lysine deficient protein kinase 1 antibody|WNK1 antibody|WNK1_HUMAN antibody|Anti-WNK1 antibody (ab53151)
SCBT cat No: To be updated

 

Name

WNK1 (phospho Thr60) Polyclonal Antibody

Catalogue No.

STJ90776

Reactivity

Human, Mouse, Rat

Specificity

Phospho-WNK1 (T60) Polyclonal Antibody detects endogenous levels of WNK1 protein only when phosphorylated at T60.

Immunogen

Synthesized phospho-peptide derived from WNK1 (phospho Thr60) at AA range 40-120

Host

Rabbit

Applications

WB, IHC, ELISA

Recommended dilution

WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:5000;

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

230 kDa

Formulation

WNK1 (phospho Thr60) Antibody was tube-contained. Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Concentration

1 mg/ml

Purification

WNK1 (phospho Thr60) Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage

-20 Celsius degree. Avoid repeated freeze/thaw cycles.

Alternative antibody names

Serine/threonine-protein kinase WNK1 antibody, Erythrocyte 65 kDa protein antibody, p65 antibody, Kinase deficient protein antibody, Protein kinase lysine-deficient 1 antibody, Protein kinase with no lysine 1 antibody, hWNK1 antibody

Database links

Human UniProt/Swiss-Prot:Q9H4A3;Mouse UniPort/Swiss-Prot: P83741;Rat UniProt/Swiss-Port: Q9JIH7;Human Entrez Gene: 65125;Mouse Entrez Gene: 232341;Rat Entrez Gene: Rn.27409

Protein names

Serine/threonine-protein kinase WNK1 , Erythrocyte 65 kDa protein , p65 , Kinase deficient protein , Protein kinase lysine-deficient 1 , Protein kinase with no lysine 1 , hWNK1

Protein function

Serine/threonine kinase which plays an important role in the regulation of electrolyte homeostasis, cell signaling, survival, and proliferation. Acts as an activator and inhibitor of sodium-coupled chloride cotransporters and potassium-coupled chloride cotransporters respectively. Activates SCNN1A, SCNN1B, SCNN1D and SGK1. Controls sodium and chloride ion transport by inhibiting the activity of WNK4, by either phosphorylating the kinase or via an interaction between WNK4 and the autoinhibitory domain of WNK1. WNK4 regulates the activity of the thiazide-sensitive Na-Cl cotransporter, SLC12A3, by phosphorylation. WNK1 may also play a role in actin cytoskeletal reorganization. Phosphorylates NEDD4L. Acts as a scaffold to inhibit SLC4A4, SLC26A6 as well as CFTR activities and surface expression, recruits STK39 which mediates the inhibition (By similarity). / ATP + a protein = ADP + a phosphoprotein. / Mg2+ / By hypertonicity. Activation requires autophosphorylation of Ser-382. Phosphorylation of Ser-378 also promotes increased activity (By similarity).

Protein tissue specificity

Widely expressed, with highest levels observed in the testis, heart, kidney and skeletal muscle. Isoform 3 is kidney-specific and specifically expressed in the distal convoluted tubule (DCT) and connecting tubule (CNT) of the nephron.

Involvement in disease

Pseudohypoaldosteronism 2C (PHA2C) [MIM:614492]: An autosomal dominant disorder characterized by severe hypertension, hyperkalemia, hyperchloremia, mild hyperchloremic metabolic acidosis in some cases, and correction of physiologic abnormalities by thiazide diuretics. . Note: The disease is caused by mutations affecting the gene represented in this entry.; Neuropathy, hereditary sensory and autonomic, 2A (HSAN2A) [MIM:201300]: A form of hereditary sensory and autonomic neuropathy, a genetically and clinically heterogeneous group of disorders characterized by degeneration of dorsal root and autonomic ganglion cells, and by sensory and/or autonomic abnormalities. HSAN2A is an autosomal recessive disorder characterized by impairment of pain, temperature and touch sensation, onset of symptoms in infancy or early childhood, occurrence of distal extremity pathologies (paronychia, whitlows, ulcers, and Charcot joints), frequent amputations, sensory loss that affects all modalities of sensation (lower and upper limbs and perhaps the trunk as well), absence or diminution of tendon reflexes (usually in all limbs), minimal autonomic dysfunction, absence of sensory nerve action potentials, and virtual absence of myelinated fibers with decreased numbers of unmyelinated fibers in sural nerves. . Note: The disease is caused by mutations affecting the gene represented in this entry.

Protein sequence and domain

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. WNK subfamily. / Contains 1 protein kinase domain.

Protein post-translational modifications

O-glycosylated. / Ubiquitinated in vitro by the BCR(KLHL3) complex and in vivo by a BCR(KLHL2) complex, leading to proteasomal degradation.

Protein cellular localization

Cytoplasm

Research area

All research areas>Kinases and Phosphatases>WNK
(View all antibody categories related to Kinases and Phosphatases)

Note

AntibodyPlus can customize WNK1 (phospho Thr60) Antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provide antibody trial sample for your own antibody validation and collects antibody reviews.

Supplier

St John’s Laboratory Ltd.

Product type

Primary antibody

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