Rabbit Polyclonal Phospho-YAP (S127) antibody (STJ90868)

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Reactivity: Human, Mouse, Rat, Monkey
Applications: WB, IF
Conjugation: Unconjugated
Supplier: St John’s Laboratory Ltd.

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Rabbit Polyclonal Phospho-YAP (S127) antibody (STJ90868)

Supplier: St John’s Laboratory Ltd.

Recommended applications: WB, IHC, ELISA

Recommended dilution: WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:40000;

Recommended protocols: check protocols

Image descriptions:

Click or hover above images to see image description for YAP (phospho Ser127) Polyclonal Antibody.

Alternative names:

Check alternative names for the antibody

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YAP1 antibody, YAP65 antibody,|65 kDa Yes associated protein antibody|65 kDa Yes-associated protein antibody|COB1 antibody|YAp 1 antibody|YAP 65 antibody|YAP antibody|YAP1 antibody|YAP1_HUMAN antibody|YAP2 antibody|YAP65 antibody|yes -associated protein delta antibody|Yes associated protein 1 65kDa antibody|Yes associated protein 1 antibody|Yes associated protein 2 antibody|yes associated protein beta antibody|YKI antibody|Yorkie homolog antibody|Anti-YAP1 antibody [EP1674Y] (ab52771)
SCBT cat No: To be updated

Name

YAP (phospho Ser127) Polyclonal Antibody

Catalogue No.

STJ90868

Reactivity

Human, Mouse, Rat, Monkey

Specificity

Phospho-YAP (S127) Polyclonal Antibody detects endogenous levels of YAP protein only when phosphorylated at S127.

Immunogen

Synthesized phospho-peptide derived from YAP (phospho Ser127) at AA range 70-150

Host

Rabbit

Applications

WB, IHC, ELISA

Recommended dilution

WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:40000;

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

65 kDa

Formulation

YAP (phospho Ser127) Antibody was tube-contained. Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Concentration

1 mg/ml

Purification

YAP (phospho Ser127) Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage

-20 Celsius degree. Avoid repeated freeze/thaw cycles.

Alternative antibody names

Transcriptional coactivator YAP1 antibody, Yes-associated protein 1 antibody, Protein yorkie homolog antibody, Yes-associated protein YAP65 homolog antibody

Database links

Human UniProt/Swiss-Prot:P46937;Mouse UniPort/Swiss-Prot: P46938;Rat UniProt/Swiss-Port: Q2EJA0;Human Entrez Gene: 10413;Mouse Entrez Gene: 22601;Rat Entrez Gene: Rn.162978

Protein names

Transcriptional coactivator YAP1 , Yes-associated protein 1 , Protein yorkie homolog , Yes-associated protein YAP65 homolog

Protein function

Transcriptional regulator which can act both as a coactivator and a corepressor and is the critical downstream regulatory target in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis . The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ . Plays a key role in tissue tension and 3D tissue shape by regulating cortical actomyosin network formation. Acts via ARHGAP18, a Rho GTPase activating protein that suppresses F-actin polymerization . Plays a key role to control cell proliferation in response to cell contact. Phosphorylation of YAP1 by LATS1/2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration . The presence of TEAD transcription factors are required for it to stimulate gene expression, cell growth, anchorage-independent growth, and epithelial mesenchymal transition (EMT) induction . / Isoform 2: Isoform 2 and isoform 3 can activate the C-terminal fragment (CTF) of ERBB4 (isoform 3).

Protein tissue specificity

Increased expression seen in some liver and prostate cancers. Isoforms lacking the transactivation domain found in striatal neurons of patients with Huntington disease (at protein level).

Involvement in disease

Coloboma, ocular, with or without hearing impairment, cleft lip/palate, and/or mental retardation (COB1) [MIM:120433]: An autosomal dominant disease characterized by uveal colobomata, microphthalmia, cataract and cleft lip/palate. Considerable variability is observed among patients, uveal colobomata being the most constant feature. Some patients manifest mental retardation of varying degree and/or sensorineural, mid-frequency hearing loss. . Note: The disease is caused by mutations affecting the gene represented in this entry.

Protein sequence and domain

The first coiled-coil region mediates most of the interaction with TEAD transcription factors. / Belongs to the YAP1 family. / Contains 2 WW domains.

Protein post-translational modifications

Phosphorylated by LATS1 and LATS2; leading to cytoplasmic translocation and inactivation . Phosphorylated by ABL1; leading to YAP1 stabilization, enhanced interaction with TP73 and recruitment onto proapoptotic genes; in response to DNA damage . Phosphorylation at Ser-400 and Ser-403 by CK1 is triggered by previous phosphorylation at Ser-397 by LATS proteins and leads to YAP1 ubiquitination by SCF(beta-TRCP) E3 ubiquitin ligase and subsequent degradation . Phosphorylated at Thr-119, Ser-138, Thr-154, Ser-367 and Thr-412 by MAPK8/JNK1 and MAPK9/JNK2, which is required for the regukation of apoptosis by YAP1 . / Ubiquitinated by SCF(beta-TRCP) E3 ubiquitin ligase.

Protein cellular localization

Cytoplasm / Nucleus

Research area

All research areas>Transcription Regulators>YAP
(View all antibody categories related to Transcription Regulators)

Note

AntibodyPlus can customize YAP (phospho Ser127) Antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provide antibody trial sample for your own antibody validation and collects antibody reviews.

Supplier

St John’s Laboratory Ltd.

Product type

Primary antibody

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Immunofluorescence analysis of Rat heart tissue

1: YAP (phospho Ser127) Polyclonal Antibody(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunofluorescence analysis of Rat heart tissue

1: YAP (phospho Ser127) Polyclonal Antibody(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.


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