Rabbit polyclonal PIAS2 antibody (A5654)

$69.00$259.00

Reactivity: Human,Mouse,Rat
Applications: WB,IHC
Conjugation: Unconjugated
Supplier: ABclonal Inc.

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Rabbit polyclonal PIAS2 antibody (A5654)

Supplier: ABclonal Inc.

Recommended applications: WB,IHC

Recommended dilution:

WB 1:500 – 1:2000 IHC 1:50 – 1:200

Recommended protocols: check protocols

Image descriptions:

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Alternative names:

Check alternative names for the antibody

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DIP antibody, MIZ antibody, MIZ1 antibody, SIZ2 antibody, ARIP3 antibody, PIASX antibody, ZMIZ4 antibody, PIASX-BETA antibody, PIASX-ALPHA antibody
Androgen receptor interacting protein 3 antibody|Androgen receptor-interacting protein 3 antibody|ARIP3 antibody|DAB2 interacting protein antibody|DAB2-interacting protein antibody|DIP antibody|E3 SUMO protein ligase PIAS2 antibody|E3 SUMO-protein ligase PIAS2 antibody|MIZ antibody|Miz1 antibody|Msx interacting zinc finger protein antibody|Msx-interacting zinc finger protein antibody|PIAS NY protein antibody|PIAS-NY protein antibody|PIAS2 antibody|PIAS2_HUMAN antibody|PIASX antibody|PIASX-ALPHA antibody|PIASX-BETA antibody|Protein inhibitor of activated STAT x antibody|Protein inhibitor of activated STAT, 2 antibody|Protein inhibitor of activated STAT2 antibody|SIZ2 antibody|Zinc finger, MIZ-type containing 4 antibody|ZMIZ4 antibody|Anti-PIAS2 antibody (ab155556)

SCBT cat No: sc-18245|sc-166494|sc-30879|

Name

Rabbit polyclonal PIAS2 antibody

Catalogue No.

A5654

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant protein of human PIAS2

Host

Rabbit

Applications

WB, IHC

Recommended dilution

WB 1:500 – 1:2000
IHC 1:50 – 1:200

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

Predicted: 68kDa/Observed: Refer to Figures

Formulation

PIAS2 antibody was tube-contained.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Purification

PIAS2 antibody was purified using affinity purification.

Storage

Store at -20 Celsius degree. Avoid freeze / thaw cycles.

Alternative antibody names

DIP antibody, MIZ antibody, MIZ1 antibody, SIZ2 antibody, ARIP3 antibody, PIASX antibody, ZMIZ4 antibody, PIASX-BETA antibody, PIASX-ALPHA antibody

Database links

Human UniProt/Swiss-Prot:O75928

Protein names

DIP, MIZ, MIZ1, SIZ2, ARIP3, PIASX, ZMIZ4, PIASX-BETA, PIASX-ALPHA

Protein function

Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulator in various cellular pathways, including the STAT pathway, the p53 pathway and the steroid hormone signaling pathway. The effects of this transcriptional coregulation, transactivation or silencing may vary depending upon the biological context and the PIAS2 isoform studied. However, it seems to be mostly involved in gene silencing. Binds to sumoylated ELK1 and enhances its transcriptional activity by preventing recruitment of HDAC2 by ELK1, thus reversing SUMO-mediated repression of ELK1 transactivation activity. Isoform PIAS2-beta, but not isoform PIAS2-alpha, promotes MDM2 sumoylation. Isoform PIAS2-alpha promotes PARK7 sumoylation. Isoform PIAS2-beta promotes NCOA2 sumoylation more efficiently than isoform PIAS2-alpha. Isoform PIAS2-alpha sumoylates PML at’Lys-65′ and ‘Lys-160’.

Protein tissue specificity

Mainly expressed in testis. Isoform 3 is expressed predominantly in adult testis, weakly in pancreas, embryonic testis and sperm, and at very low levels in other organs.

Protein sequence and domain

The LXXLL motif is a transcriptional coregulator signature.Belongs to the PIAS family.; Contains 1 PINIT domain.; Contains 1 SAP domain.; Contains 1 SP-RING-type zinc finger.

Protein post-translational modifications

Sumoylated.

Protein cellular localization

Nucleus speckle . Nucleus, PML body . Nucleus . Note: Colocalizes at least partially with promyelocytic leukemia nuclear bodies (PML NBs) . Colocalizes with SUMO1 in nuclear granules (By similarity).

Background

This gene encodes a member of the protein inhibitor of activated STAT (PIAS) family. PIAS proteins function as SUMO E3 ligases and play important roles in many cellular processes by mediating the sumoylation of target proteins. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. Isoforms of the encoded protein enhance the sumoylation of specific target proteins including the p53 tumor suppressor protein, c-Jun, and the androgen receptor. A pseudogene of this gene is located on the short arm of chromosome 4. The symbol MIZ1 has also been associated with ZBTB17 which is a different gene located on chromosome 1.

Research area

All research areas>Signaling Intermediates>PIAS
(View all antibody categories related to Signaling Intermediates)

Note

AntibodyPlus can customize PIAS2 antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provides antibody trial sample for your own antibody validation.

Product type

Primary antibody

Supplier

ABclonal Inc.

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