Rabbit polyclonal PIK3C2A antibody (A8526)

$69.00$259.00

Reactivity: Human,Mouse,Rat
Applications: WB,IHC
Conjugation: Unconjugated
Supplier: ABclonal Inc.

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Rabbit polyclonal PIK3C2A antibody (A8526)

Supplier: ABclonal Inc.

Recommended applications: WB,IHC

Recommended dilution:

WB 1:500 – 1:2000 IHC 1:50 – 1:100

Recommended protocols: check protocols

Image descriptions:

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Alternative names:

Check alternative names for the antibody

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CPK antibody, PI3-K-C2A antibody, PI3-K-C2(ALPHA) antibody
C2 containing phosphatidylinositol kinase antibody|C2 domain containing alpha polypeptide antibody|Class 2 alpha polypeptide antibody|CPK antibody|Cpk m antibody|DKFZp686L193 antibody|MGC142218 antibody|OTTHUMP00000231763 antibody|P3C2A_HUMAN antibody|Phosphatidylinositol 3 kinase class 2 alpha antibody|Phosphatidylinositol-4-phosphate 3-kinase C2 domain-containing subunit alpha antibody|Phosphoinositide 3 kinase class 2 alpha antibody|Phosphoinositide 3 kinase, class 2, alpha polypeptide antibody|Phosphoinositide 3-Kinase-C2-alpha antibody|PI3-K-C2(ALPHA) antibody|PI3-K-C2A antibody|PI3K-C2-alpha antibody|PI3K-C2alpha antibody|PI3KC 2 alpha antibody|PIK3C 2A antibody|Pik3c2a antibody|PtdIns-3-kinase C2 subunit alpha antibody|Anti-PI 3 Kinase Class 2A antibody – C-terminal (ab154583)

SCBT cat No: sc-136298|sc-365290|sc-67306|sc-48639|sc-100407|sc-136031|

Name

Rabbit polyclonal PIK3C2A antibody

Catalogue No.

A8526

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant protein of human PIK3C2A

Host

Rabbit

Applications

WB, IHC

Recommended dilution

WB 1:500 – 1:2000
IHC 1:50 – 1:100

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

Predicted: 191kDa/Observed: Refer to figures

Formulation

PIK3C2A antibody was tube-contained.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Purification

PIK3C2A antibody was purified using affinity purification.

Storage

Store at -20 Celsius degree. Avoid freeze / thaw cycles.

Alternative antibody names

CPK antibody, PI3-K-C2A antibody, PI3-K-C2(ALPHA) antibody

Database links

Human UniProt/Swiss-Prot:O00443

Protein names

CPK, PI3-K-C2A, PI3-K-C2(ALPHA)

Protein function

Generates phosphatidylinositol 3-phosphate (PtdIns3P) and phosphatidylinositol 3,4-bisphosphate (PtdIns(3,4)P2) that act as second messengers. Has a role in several intracellular trafficking events. Functions in insulin signaling and secretion. Required for translocation of the glucose transporter SLC2A4/GLUT4 to the plasma membrane and glucose uptake in response to insulin-mediated RHOQ activation. Regulates insulin secretion through two different mechanisms: involved in glucose-induced insulin secretion downstream of insulin receptor in a pathway that involves AKT1 activation and TBC1D4/AS160 phosphorylation, and participates in the late step of insulin granule exocytosis probably in insulin granule fusion. Synthesizes PtdIns3P in response to insulin signaling. Functions in clathrin-coated endocytic vesicle formation and distribution. Regulates dynamin-independent endocytosis, probably by recruiting EEA1 to internalizing vesicles. In neurosecretory cells synthesizes PtdIns3P on large dense core vesicles. Participates in calcium induced contraction of vascular smooth muscle by regulating myosin light chain (MLC) phosphorylation through a mechanism involving Rho kinase-dependent phosphorylation of the MLCP-regulatory subunit MYPT1. May play a role in the EGF signaling cascade. May be involved in mitosis and UV-induced damage response. Required for maintenance of normal renal structure and function by supporting normal podocyte function.

Protein tissue specificity

Expressed in columnar and transitional epithelia, mononuclear cells, smooth muscle cells, and endothelial cells lining capillaries and small venules (at protein level). Ubiquitously expressed, with highest levels in heart, placenta and ovary, and lowest levels in the kidney. Detected at low levels in islets of Langerhans from type 2 diabetes mellitus individuals.

Protein sequence and domain

Belongs to the PI3/PI4-kinase family.; Contains 1 C2 domain.; Contains 1 C2 PI3K-type domain.; Contains 1 PI3K-RBD domain.; Contains 1 PI3K/PI4K domain.; Contains 1 PIK helical domain.; Contains 1 PX (phox homology) domain.

Protein post-translational modifications

Phosphorylated upon insulin stimulation; which may lead to enzyme activation (By similarity). Phosphorylated on Ser-259 during mitosis and upon UV irradiation; which does not change enzymatic activity but leads to proteasomal degradation. Ser-259 phosphorylation may be mediated by CDK1 or JNK, depending on the physiological state of the cell.

Protein cellular localization

Cell membrane . Golgi apparatus . Cytoplasmic vesicle, clathrin-coated vesicle . Nucleus . Cytoplasm . Note: Inserts preferentially into membranes containing PtdIns(4,5)P2 . Associated with RNA-containing structures .

Background

The protein encoded by this gene belongs to the phosphoinositide 3-kinase (PI3K) family. PI3-kinases play roles in signaling pathways involved in cell proliferation, oncogenic transformation, cell survival, cell migration, and intracellular protein trafficking. This protein contains a lipid kinase catalytic domain as well as a C-terminal C2 domain, a characteristic of class II PI3-kinases. C2 domains act as calcium-dependent phospholipid binding motifs that mediate translocation of proteins to membranes, and may also mediate protein-protein interactions. The PI3-kinase activity of this protein is not sensitive to nanomolar levels of the inhibitor wortmanin. This protein was shown to be able to be activated by insulin and may be involved in integrin-dependent signaling.

Note

AntibodyPlus can customize PIK3C2A antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provides antibody trial sample for your own antibody validation.

Product type

Primary antibody

Supplier

ABclonal Inc.

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