Rabbit polyclonal PIWIL4 antibody (A3144)

$69.00$259.00

Reactivity: Human,Mouse,Rat
Applications: WB,IHC,IF
Conjugation: Unconjugated
Supplier: ABclonal Inc.

Clear
-$50 as credit for submitting review for primary antibody
Shipping notice:
Product will be delivered in 6 to 8 Business Days

Rabbit polyclonal PIWIL4 antibody (A3144)

Supplier: ABclonal Inc.

Recommended applications: WB,IHC,IF

Recommended dilution:

WB 1:500 – 1:2000 IHC 1:50 – 1:200 IF 1:50 – 1:200

Recommended protocols: check protocols

Image descriptions:

Click or hover above images to see image description for Rabbit polyclonal PIWIL4 antibody.

Alternative names:

Check alternative names for the antibody

Expand

HIWI2 antibody, MIWI2 antibody
DKFZp686P01248 antibody|FLJ36156 antibody|HILI 2 antibody|HILI2 antibody|HIWI 2 antibody|HIWI2 antibody|Miwi 2 protein antibody|Miwi2 antibody|PIWI antibody|Piwi like 2 antibody|Piwi like 4 (Drosophila) antibody|Piwi like 4 antibody|Piwi like protein 4 antibody|PIWI like protein antibody|Piwi like RNA mediated gene silencing 4 antibody|Piwi-like protein 4 antibody|PIWIL 4 antibody|Piwil4 antibody|PIWL4_HUMAN antibody|Anti-PIWIL4 antibody (ab21869)

SCBT cat No: sc-517215|sc-68932|sc-67593|

Name

Rabbit polyclonal PIWIL4 antibody

Catalogue No.

A3144

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant protein of human PIWIL4

Host

Rabbit

Applications

WB, IHC, IF

Recommended dilution

WB 1:500 – 1:2000
IHC 1:50 – 1:200
IF 1:50 – 1:200

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

Predicted: 97kDa/Observed: Refer to Figures

Formulation

PIWIL4 antibody was tube-contained.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Purification

PIWIL4 antibody was purified using affinity purification.

Storage

Store at -20 Celsius degree. Avoid freeze / thaw cycles.

Alternative antibody names

HIWI2 antibody, MIWI2 antibody

Database links

Human UniProt/Swiss-Prot:Q7Z3Z4

Protein names

HIWI2, MIWI2

Protein function

Plays a central role during spermatogenesis by repressing transposable elements and preventing their mobilization, which is essential for the germline integrity. Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and governs the methylation and subsequent repression of transposons. Directly binds piRNAs, a class of 24 to 30 nucleotide RNAs that are generated by a Dicer-independent mechanism and are primarily derived from transposons and other repeated sequence elements. Associates with secondary piRNAs antisense and PIWIL2/MILI is required for such association. The piRNA process acts upstream of known mediators of DNA methylation. Participates in a piRNA amplification loop. Besides their function in transposable elements repression, piRNAs are probably involved in other processes during meiosis such as translation regulation (By similarity). May be involved in the chromatin-modifying pathway by inducing ‘Lys-9’ methylation of histone H3 at some loci .

Protein tissue specificity

Expressed in testis. According to PubMed:17544373, it is ubiquitously expressed.

Protein sequence and domain

Belongs to the argonaute family. Piwi subfamily.; Contains 1 PAZ domain.; Contains 1 Piwi domain.

Protein post-translational modifications

Arginine methylation by PRMT5 is required for the interaction with Tudor domain-containing protein (TDRD1, TDRKH/TDRD2 and TDRD9) and subsequent localization to the meiotic nuage, also named P granule.

Protein cellular localization

Nucleus . Cytoplasm . Note: Probable component of the meiotic nuage, also named P granule, a germ-cell-specific organelle required to repress transposon activity during meiosis. PIWIL2/MILI is required for nuclear localization (By similarity).

Background

PIWIL4 belongs to the Argonaute family of proteins, which function in development and maintenance of germline stem cells. Plays a central role during spermatogenesis by repressing transposable elements and prevent their mobilization, which is essential for the germline integrity. Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and govern the methylation and subsequent repression of transposons. Directly binds piRNAs, a class of 24 to 30 nucleotide RNAs that are generated by a Dicer-independent mechanism and are primarily derived from transposons and other repeated sequence elements. Associates with secondary piRNAs antisense and PIWIL2/MILI is required for such association.

Note

AntibodyPlus can customize PIWIL4 antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provides antibody trial sample for your own antibody validation.

Product type

Primary antibody

Supplier

ABclonal Inc.

Reviews


There are no reviews yet.

Only logged in customers who have purchased this product may leave a review

There are no reviews for this product yet.
By submitting a review, get following benefits:

1. Receive $50 ABcoins as credit for each review.
2. First trial sample order will be fully refunded as credit.
3. Have a chance to win a $50 amazon gift card!

view database submit reviews

There is no extra validation for this product yet.
Check other extra validated antibodies below:



Welcome to AntibodyPlus!