Rabbit Polyclonal PKM2 antibody (STJ95142)

$99.00$319.00

Reactivity: Human, Mouse, Rat
Applications: WB, IHC, IF
Conjugation: Unconjugated
Supplier: St John’s Laboratory Ltd.

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Rabbit Polyclonal PKM2 antibody (STJ95142)

Supplier: St John’s Laboratory Ltd.

Recommended applications: WB, ELISA

Recommended dilution: WB 1:500-1:2000; ELISA 1:10000;

Recommended protocols: check protocols

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Alternative names:

Check alternative names for the antibody

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SCBT cat No: sc-365684|sc-65176|sc-292640|sc-135048|sc-100538|

Name

PKM2 Polyclonal Antibody

Catalogue No.

STJ95142

Reactivity

Human, Mouse, Rat

Specificity

PKM2 Polyclonal Antibody detects endogenous levels of PKM2 protein.

Immunogen

Synthesized peptide derived from the Internal region of human PKM2

Host

Rabbit

Applications

WB, ELISA

Recommended dilution

WB 1:500-1:2000; ELISA 1:10000;

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

58 kDa

Formulation

PKM2 Antibody was tube-contained. Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Concentration

1 mg/ml

Purification

PKM2 Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage

-20 Celsius degree. Avoid repeated freeze/thaw cycles.

Alternative antibody names

Pyruvate kinase PKM antibody, Cytosolic thyroid hormone-binding protein antibody, CTHBP antibody, Opa-interacting protein 3 antibody, OIP-3 antibody, Pyruvate kinase 2/3 antibody, Pyruvate kinase muscle isozyme antibody, Thyroid hormone-binding protein 1 antibody, THBP1 antibody, Tumor M2-PK antibody, p58 antibody

Database links

Human UniProt/Swiss-Prot:P14618;Mouse UniPort/Swiss-Prot: P52480;Rat UniProt/Swiss-Port: P11980;Human Entrez Gene: 5315;Mouse Entrez Gene: 18746;Rat Entrez Gene: Rn.1556

Protein names

Pyruvate kinase PKM , Cytosolic thyroid hormone-binding protein , CTHBP , Opa-interacting protein 3 , OIP-3 , Pyruvate kinase 2/3 , Pyruvate kinase muscle isozyme , Thyroid hormone-binding protein 1 , THBP1 , Tumor M2-PK , p58

Protein function

Glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. Stimulates POU5F1-mediated transcriptional activation. Plays a general role in caspase independent cell death of tumor cells. The ratio betwween the highly active tetrameric form and nearly inactive dimeric form determines whether glucose carbons are channeled to biosynthetic processes or used for glycolytic ATP production. The transition between the 2 forms contributes to the control of glycolysis and is important for tumor cell proliferation and survival. / ATP + pyruvate = ADP + phosphoenolpyruvate. / Mg2+ / K+ / Isoform M2 is allosterically activated by D-fructose 1,6-bisphosphate (FBP). Inhibited by oxalate and 3,3′,5-triiodo-L-thyronine (T3). The activity of the tetrameric form is inhibited by PML. Selective binding to tyrosine-phosphorylated peptides releases the allosteric activator FBP, leading to inhibition of PKM enzymatic activity, this diverts glucose metabolites from energy production to anabolic processes when cells are stimulated by certain growth factors. Glycolytic flux are highly dependent on de novo biosynthesis of serine and glycine, and serine is a natural ligand and allosteric activator of isoform M2. / 2.7 mM for phosphoenolpyruvate (at 32 degrees Celsius, pH 8.0) / 0.17 mM for phosphoenolpyruvate (in the presence of 2 mM FBP, at 32 degrees Celsius, pH 8.0) / 0.34 mM for ADP (at 32 degrees Celsius, pH 8.0) / 0.24 mM for ADP (in the presence of 2 mM FBP, at 32 degrees Celsius, pH 8.0) / 0.13 mM for phosphoenolpyruvate (in the presence of 2 mM FBP, at 25 degrees Celsius) / 0.63 mM for ADP (in the presence of 2 mM FBP, at 25 degrees Celsius) / Optimum pH for T3 binding is 6.0-6.5. Increase in pH causes T3 binding to drop, does not bind T3 above pH 9.0 or below pH 5.0. /

Protein tissue specificity

Specifically expressed in proliferating cells, such as embryonic stem cells, embryonic carcinoma cells, as well as cancer cells.

Protein sequence and domain

Belongs to the pyruvate kinase family.

Protein post-translational modifications

ISGylated. / Under hypoxia, hydroxylated by EGLN3. / Acetylation at Lys-305 is stimulated by high glucose concentration, it decreases enzyme activity and promotes its lysosomal-dependent degradation via chaperone-mediated autophagy.

Protein cellular localization

Cytoplasm / Nucleus

Research area

All research areas>Synthesis and Degradation>PKM2
(View all antibody categories related to Synthesis and Degradation)

Note

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Supplier

St John’s Laboratory Ltd.

Product type

Primary antibody

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Immunofluorescence analysis of Rat lung tissue

1: PKM2 Polyclonal Antibody(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunofluorescence analysis of Rat lung tissue

1: PKM2 Polyclonal Antibody(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunofluorescence analysis of Rat kidney tissue

1: PKM2 Polyclonal Antibody(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunofluorescence analysis of Rat kidney tissue

1: PKM2 Polyclonal Antibody(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunofluorescence analysis of Rat spleen tissue

1: PKM2 Polyclonal Antibody(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunofluorescence analysis of Rat spleen tissue

1: PKM2 Polyclonal Antibody(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunohistochemical analysis of paraffin embedded Human uterus tissue

1: PKM2 Polyclonal Antibody was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Human uterus cancer tissue

1: PKM2 Polyclonal Antibody was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Human colon tissue

1: PKM2 Polyclonal Antibody was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Human stomach tissue

1: PKM2 Polyclonal Antibody was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Human stomach cancer tissue

1: PKM2 Polyclonal Antibody was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.


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