Rabbit polyclonal POLB antibody (A1681)

$69.00$259.00

Reactivity: Human,Mouse,Rat
Applications: WB,IHC,IF
Conjugation: Unconjugated
Supplier: ABclonal Inc.

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Rabbit polyclonal POLB antibody (A1681)

Supplier: ABclonal Inc.

Recommended applications: WB,IHC,IF

Recommended dilution:

WB 1:200 – 1:1000 IHC 1:50 – 1:200 IF 1:50 – 1:200

Recommended protocols: check protocols

Image descriptions:

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Alternative names:

Check alternative names for the antibody

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MGC125976 antibody, POLB antibody
DNA directed DNA polymerase beta antibody|DNA pol beta antibody|DNA polymerase beta antibody|DNA polymerase beta subunit antibody|DPOLB_HUMAN antibody|MGC125976 antibody|Pol B antibody|Pol beta antibody|POLB antibody|Polymerase (DNA directed) beta antibody|Anti-DNA Polymerase beta antibody (ab26343)

SCBT cat No: sc-376581|sc-48819|sc-5927|sc-5925|

Name

Rabbit polyclonal POLB antibody

Catalogue No.

A1681

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant protein of human POLB

Host

Rabbit

Applications

WB, IHC, IF

Recommended dilution

WB 1:200 – 1:1000
IHC 1:50 – 1:200
IF 1:50 – 1:200

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

Predicted: 38kDa/Observed: Refer to Figures

Formulation

POLB antibody was tube-contained.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Purification

POLB antibody was purified using affinity purification.

Storage

Store at -20 Celsius degree. Avoid freeze / thaw cycles.

Alternative antibody names

MGC125976 antibody, POLB antibody

Database links

Human UniProt/Swiss-Prot:P06746

Protein names

MGC125976, POLB

Protein function

Repair polymerase that plays a key role in base-excision repair. Has 5′-deoxyribose-5-phosphate lyase (dRP lyase) activity that removes the 5′ sugar phosphate and also acts as a DNA polymerase that adds one nucleotide to the 3′ end of the arising single-nucleotide gap. Conducts ‘gap-filling’ DNA synthesis in a stepwise distributive fashion rather than in a processive fashion as for other DNA polymerases.

Protein sequence and domain

Residues 239-252 form a flexible loop which appears to affect the polymerase fidelity.Belongs to the DNA polymerase type-X family.

Protein post-translational modifications

Methylation by PRMT6 stimulates the polymerase activity by enhancing DNA binding and processivity.; Ubiquitinated at Lys-41, Lys-61 and Lys-81: monoubiquitinated by HUWE1/ARF-BP1. Monoubiquitinated protein is then the target of STUB1/CHIP, which catalyzes polyubiquitination from monoubiquitin, leading to degradation by the proteasome. USP47 mediates the deubiquitination of monoubiquitinated protein, preventing polyubiquitination by STUB1/CHIP and its subsequent degradation.

Protein cellular localization

Nucleus. Cytoplasm. Note: Cytoplasmic in normal conditions. Translocates to the nucleus following DNA damage.

Background

The protein encoded by this gene is a DNA polymerase involved in base excision and repair, also called gap-filling DNA synthesis. The encoded protein, acting as a monomer, is normally found in the cytoplasm, but it translocates to the nucleus upon DNA damage. Several transcript variants of this gene exist, but the full-length nature of only one has been described to date. [provided by RefSeq, Sep 2011]

Research area

All research areas>Transcription Regulators>DNA Polymerase
(View all antibody categories related to Transcription Regulators)

Note

AntibodyPlus can customize POLB antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provides antibody trial sample for your own antibody validation.

Product type

Primary antibody

Supplier

ABclonal Inc.

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