Rabbit polyclonal POU5F1 antibody (A7920)

$69.00$259.00

Reactivity: Human,Mouse,Rat
Applications: WB,IHC
Conjugation: Unconjugated
Supplier: ABclonal Inc.

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Rabbit polyclonal POU5F1 antibody (A7920)

Supplier: ABclonal Inc.

Recommended applications: WB,IHC

Recommended dilution:

WB 1:500 – 1:2000 IHC 1:50 – 1:200

Recommended protocols: check protocols

Image descriptions:

Click or hover above images to see image description for Rabbit polyclonal POU5F1 antibody.

Alternative names:

Check alternative names for the antibody

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OCT3 antibody, OCT4 antibody, OTF3 antibody, OTF4 antibody, OTF-3 antibody, Oct-3 antibody, Oct-4 antibody
Octamer binding transcription factor 4 antibody|MGC22487 antibody|Oct 3 antibody|Oct 4 antibody|Oct-3 antibody|Oct-4 antibody|OCT3 antibody|Oct4 antibody|Octamer binding protein 3 antibody|Octamer binding protein 4 antibody|Octamer binding transcription factor 3 antibody|Octamer-binding protein 3 antibody|Octamer-binding protein 4 antibody|Octamer-binding transcription factor 3 antibody|OTF 3 antibody|OTF 4 antibody|OTF-3 antibody|OTF3 antibody|OTF4 antibody|PO5F1_HUMAN antibody|POU class 5 homeobox 1 antibody|POU domain class 5 transcription factor 1 antibody|POU domain transcription factor OCT4 antibody|POU domain, class 5, transcription factor 1 antibody|POU-type homeodomain-containing DNA-binding protein antibody|POU5F1 antibody|Anti-Oct4 antibody – ChIP Grade (ab19857)

SCBT cat No: sc-101534|sc-365509|sc-5279|sc-8629|sc-514295|sc-9081|sc-25401|sc-8628|sc-8630|

Name

Rabbit polyclonal POU5F1 antibody

Catalogue No.

A7920

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide of human POU5F1

Host

Rabbit

Applications

WB, IHC

Recommended dilution

WB 1:500 – 1:2000
IHC 1:50 – 1:200

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

Predicted: 39kDa/Observed: Refer to figures

Formulation

POU5F1 antibody was tube-contained.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Purification

POU5F1 antibody was purified using affinity purification.

Storage

Store at -20 Celsius degree. Avoid freeze / thaw cycles.

Alternative antibody names

OCT3 antibody, OCT4 antibody, OTF3 antibody, OTF4 antibody, OTF-3 antibody, Oct-3 antibody, Oct-4 antibody

Database links

Human UniProt/Swiss-Prot:Q01860

Protein names

OCT3, OCT4, OTF3, OTF4, OTF-3, Oct-3, Oct-4

Protein function

Transcription factor that binds to the octamer motif (5′-ATTTGCAT-3′). Forms a trimeric complex with SOX2 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency.

Protein tissue specificity

Expressed in developing brain. Highest levels found in specific cell layers of the cortex, the olfactory bulb, the hippocampus and the cerebellum. Low levels of expression in adult tissues.

Protein sequence and domain

The POU-specific domain mediates interaction with PKM.Belongs to the POU transcription factor family. Class-5 subfamily.; Contains 1 homeobox DNA-binding domain.; Contains 1 POU-specific domain.

Protein post-translational modifications

Sumoylation enhances the protein stability, DNA binding and transactivation activity. Sumoylation is required for enhanced YES1 expression (By similarity).; Ubiquitinated; undergoes ‘Lys-63’-linked polyubiquitination by WWP2 leading to proteasomal degradation.; ERK1/2-mediated phosphorylation at Ser-111 promotes nuclear exclusion and proteasomal degradation. Phosphorylation at Thr-235 and Ser-236 decrease DNA-binding and alters ability to activate transcription.

Protein cellular localization

Cytoplasm. Nucleus. Note: Expressed in a diffuse and slightly punctuate pattern.

Background

This gene encodes a transcription factor containing a POU homeodomain that plays a key role in embryonic development and stem cell pluripotency. Aberrant expression of this gene in adult tissues is associated with tumorigenesis. This gene can participate in a translocation with the Ewing’s sarcoma gene on chromosome 21, which also leads to tumor formation. Alternative splicing, as well as usage of alternative AUG and non-AUG translation initiation codons, results in multiple isoforms. One of the AUG start codons is polymorphic in human populations. Related pseudogenes have been identified on chromosomes 1, 3, 8, 10, and 12.

Research area

All research areas>Homeodomain Proteins>Oct
(View all antibody categories related to Homeodomain Proteins)

Note

AntibodyPlus can customize POU5F1 antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provides antibody trial sample for your own antibody validation.

Product type

Primary antibody

Supplier

ABclonal Inc.

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