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Rabbit polyclonal PRKAA1 antibody (A1229)
Supplier: ABclonal Inc.
Recommended applications: WB,IHC,IF
WB 1:500 – 1:2000 IHC 1:50 – 1:200 IF 1:50 – 1:200
Recommended protocols: check protocols
Click or hover above images to see image description for Rabbit polyclonal PRKAA1 antibody.
Check alternative names for the antibodyExpand
PRKAA1 antibody,AMPK antibody,AMPKa1 antibody,MGC33776 antibody,MGC57364 antibody
5 AMP activated protein kinase alpha 1catalytic subunit antibody|5 AMP activated protein kinase catalytic alpha 1 chain antibody|5′ AMP activated protein kinase catalytic subunit alpha 1 antibody|5′-AMP-activated protein kinase catalytic subunit alpha-1 antibody|AAPK1 antibody|AAPK1_HUMAN antibody|ACACA kinase antibody|acetyl CoA carboxylase kinase antibody|AI194361 antibody|AI450832 antibody|AL024255 antibody|AMP -activate kinase alpha 1 subunit antibody|AMP-activated protein kinase, catalytic, alpha -1 antibody|AMPK 1 antibody|AMPK alpha 1 antibody|AMPK alpha 1 chain antibody|AMPK antibody|AMPK subunit alpha-1 antibody|AMPK1 antibody|AMPKa1 antibody|AMPKalpha1 antibody|C130083N04Rik antibody|cb116 antibody|EC 220.127.116.11 antibody|HMG CoA reductase kinase antibody|HMGCR kinase antibody|hormone sensitive lipase kinase antibody|Hydroxymethylglutaryl CoA reductase kinase antibody|im:7154392 antibody|kinase AMPK alpha1 antibody|MGC33776 antibody|MGC57364 antibody|OTTHUMP00000161795 antibody|OTTHUMP00000161796 antibody|PRKAA 1 antibody|PRKAA1 antibody|Protein kinase AMP activated alpha 1 catalytic subunit antibody|SNF1-like protein AMPK antibody|SNF1A antibody|Tau protein kinase PRKAA1 antibody|wu:fa94c10 antibody|Anti-AMPK alpha 1 antibody [Y365] (ab32047)
SCBT cat No: sc-130394|sc-19128|sc-19126|sc-398861|sc-74461|sc-25792|sc-19129|sc-19131|sc-19133|
Rabbit polyclonal PRKAA1 antibody
|Catalogue No.|| |
Recombinant protein of human PRKAA1
WB, IHC, IF
|Recommended dilution|| |
WB 1:500 – 1:2000
|Molecular weight|| |
Predicted: 63kDa/Observed: Refer to Figures
PRKAA1 antibody was tube-contained.
PRKAA1 antibody was purified using affinity purification.
Store at -20 Celsius degree. Avoid freeze / thaw cycles.
|Alternative antibody names|| |
PRKAA1 antibody,AMPK antibody,AMPKa1 antibody,MGC33776 antibody,MGC57364 antibody
|Database links|| |
|Protein names|| |
|Protein function|| |
Catalytic subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by indirectly activating myosin. Regulates lipid synthesis by phosphorylating and inactivating lipid metabolic enzymes such as ACACA, ACACB, GYS1, HMGCR and LIPE; regulates fatty acid and cholesterol synthesis by phosphorylating acetyl-CoA carboxylase (ACACA and ACACB) and hormone-sensitive lipase (LIPE) enzymes, respectively. Regulates insulin-signaling and glycolysis by phosphorylating IRS1, PFKFB2 and PFKFB3. AMPK stimulates glucose uptake in muscle by increasing the translocation of the glucose transporter SLC2A4/GLUT4 to the plasma membrane, possibly by mediating phosphorylation of TBC1D4/AS160. Regulates transcription and chromatin structure by phosphorylating transcription regulators involved in energy metabolism such as CRTC2/TORC2, FOXO3, histone H2B, HDAC5, MEF2C, MLXIPL/ChREBP, EP300, HNF4A, p53/TP53, SREBF1, SREBF2 and PPARGC1A. Acts as a key regulator of glucose homeostasis in liver by phosphorylating CRTC2/TORC2, leading to CRTC2/TORC2 sequestration in the cytoplasm. In response to stress, phosphorylates ‘Ser-36’ of histone H2B (H2BS36ph), leading to promote transcription. Acts as a key regulator of cell growth and proliferation by phosphorylating TSC2, RPTOR and ATG1/ULK1: in response to nutrient limitation, negatively regulates the mTORC1 complex by phosphorylating RPTOR component of the mTORC1 complex and by phosphorylating and activating TSC2. In response to nutrient limitation, promotes autophagy by phosphorylating and activating ATG1/ULK1. AMPK also acts as a regulator of circadian rhythm by mediating phosphorylation of CRY1, leading to destabilize it. May regulate the Wnt signaling pathway by phosphorylating CTNNB1, leading to stabilize it. Also has tau-protein kinase activity: in response to amyloid beta A4 protein (APP) exposure, activated by CAMKK2, leading to phosphorylation of MAPT/TAU; however the relevance of such data remains unclear in vivo. Also phosphorylates CFTR, EEF2K, KLC1, NOS3 and SLC12A1.
|Protein sequence and domain|| |
The AIS (autoinhibitory sequence) region shows some sequence similarity with the ubiquitin-associated domains and represses kinase activity.Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily.; Contains 1 protein kinase domain.
|Protein post-translational modifications|| |
Ubiquitinated.; Phosphorylated at Thr-183 by STK11/LKB1 in complex with STE20-related adapter-alpha (STRADA) pseudo kinase and CAB39. Also phosphorylated at Thr-183 by CAMKK2; triggered by a rise in intracellular calcium ions, without detectable changes in the AMP/ATP ratio. CAMKK1 can also phosphorylate Thr-183, but at a much lower level. Dephosphorylated by protein phosphatase 2A and 2C (PP2A and PP2C). Phosphorylated by ULK1 and ULK2; leading to negatively regulate AMPK activity and suggesting the existence of a regulatory feedback loop between ULK1, ULK2 and AMPK. Dephosphorylated by PPM1A and PPM1B.
|Protein cellular localization|| |
Cytoplasm . Nucleus . Note: In response to stress, recruited by p53/TP53 to specific promoters.
AMP-activated protein kinase (AMPK) is highly conserved from yeast to plants and animals and plays a key role in the regulation of energy homeostasis (1). AMPK is a heterotrimeric complex composed of a catalytic alpha subunit and regulatory beta and gamma subunits, each of which is encoded by two or three distinct genes (alpha1, 2; beta1, 2; gamma1, 2, 3) (2). The kinase is activated by an elevated AMP/ATP ratio due to cellular and environmental stress, such as heat shock, hypoxia, and ischemia (1). The tumor suppressor LKB1, in association with accessory proteins STRAD and MO25, phosphorylates AMPKalpha at Thr172 in the activation loop, and this phosphorylation is required for AMPK activation (3-5). AMPKalpha is also phosphorylated at Thr258 and Ser485 (for alpha1; Ser491 for alpha2). The upstream kinase and the biological significance of these phosphorylation events have yet to be elucidated (6). The beta1 subunit is post-translationally modified by myristoylation and multi-site phosphorylation including Ser24/25, Ser96, Ser101, Ser108, and Ser182 (6,7). Phosphorylation at Ser108 of the beta1 subunit seems to be required for the activation of AMPK enzyme, while phosphorylation at Ser24/25 and Ser182 affects AMPK localization (7). Several mutations in AMPKgamma subunits have been identified, most of which are located in the putative AMP/ATP binding sites (CBS or Bateman domains). Mutations at these sites lead to reduction of AMPK activity and cause glycogen accumulation in heart or skeletal muscle (1,2). Accumulating evidence indicates that AMPK not only regulates the metabolism of fatty acids and glycogen, but also modulates protein synthesis and cell growth through EF2 and TSC2/mTOR pathways, as well as blood flow via eNOS/nNOS (1).
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|Product type|| |
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