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Rabbit polyclonal PRKAG1 antibody (A7300)
Supplier: ABclonal Inc.
Recommended applications: WB,IHC,IF
WB 1:500 – 1:2000 IHC 1:50 – 1:200 IF 1:50 – 1:100
Recommended protocols: check protocols
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Check alternative names for the antibodyExpand
5′ AMP activated protein kinase gamma 1 subunit antibody|5′ AMP activated protein kinase subunit gamma 1 antibody|5”-AMP-activated protein kinase subunit gamma-1 antibody|AAKG1_HUMAN antibody|AMP activated protein kinase noncatalytic gamma 1 subunit antibody|AMPK gamma 1 chain antibody|AMPK gamma1 antibody|AMPK subunit gamma-1 antibody|AMPKg antibody|MGC8666 antibody|PRKAG 1 antibody|PRKAG1 antibody|Protein kinase AMP activated gamma 1 non catalytic subunit antibody|protein kinase, AMP-activated, noncatalytic gamma-1 antibody|Anti-AMPK gamma 1 antibody [Y308] (ab32508)
SCBT cat No: sc-19138|sc-25793|sc-390579|
Rabbit polyclonal PRKAG1 antibody
|Catalogue No.|| |
Human, Mouse, Rat
Recombinant protein of human PRKAG1
WB, IHC, IF
|Recommended dilution|| |
WB 1:500 – 1:2000
|Molecular weight|| |
Predicted: 38kDa/Observed: Refer to figures
PRKAG1 antibody was tube-contained.
PRKAG1 antibody was purified using affinity purification.
Store at -20 Celsius degree. Avoid freeze / thaw cycles.
|Alternative antibody names|| |
|Database links|| |
|Protein names|| |
|Protein function|| |
AMP/ATP-binding subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by indirectly activating myosin. Gamma non-catalytic subunit mediates binding to AMP, ADP and ATP, leading to activate or inhibit AMPK: AMP-binding results in allosteric activation of alpha catalytic subunit (PRKAA1 or PRKAA2) both by inducing phosphorylation and preventing dephosphorylation of catalytic subunits. ADP also stimulates phosphorylation, without stimulating already phosphorylated catalytic subunit. ATP promotes dephosphorylation of catalytic subunit, rendering the AMPK enzyme inactive.
|Protein sequence and domain|| |
The AMPK pseudosubstrate motif resembles the sequence around sites phosphorylated on target proteins of AMPK, except the presence of a non-phosphorylatable residue in place of Ser. In the absence of AMP this pseudosubstrate sequence may bind to the active site groove on the alpha subunit (PRKAA1 or PRKAA2), preventing phosphorylation by the upstream activating kinase STK11/LKB1.; The CBS domains mediate binding to AMP, ADP and ATP. 2 sites bind either AMP or ATP, whereas a third site contains a tightly bound AMP that does not exchange. Under physiological conditions AMPK mainly exists in its inactive form in complex with ATP, which is much more abundant than AMP.Belongs to the 5′-AMP-activated protein kinase gamma subunit family.; Contains 4 CBS domains.
|Protein post-translational modifications|| |
Phosphorylated by ULK1 and ULK2; leading to negatively regulate AMPK activity and suggesting the existence of a regulatory feedback loop between ULK1, ULK2 and AMPK.
The protein encoded by this gene is a regulatory subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. This subunit is one of the gamma regulatory subunits of AMPK. Alternatively spliced transcript variants encoding distinct isoforms have been observed.
AntibodyPlus can customize PRKAG1 antibody according to your requirement, including bulk product size,etc. Please contact firstname.lastname@example.org. AntibodyPlus provides antibody trial sample for your own antibody validation.
|Product type|| |
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