Rabbit polyclonal PRKDC antibody (A7716)

$69.00$259.00

Reactivity: Human,Mouse
Applications: WB,IHC
Conjugation: Unconjugated
Supplier: ABclonal Inc.

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Rabbit polyclonal PRKDC antibody (A7716)

Supplier: ABclonal Inc.

Recommended applications: WB,IHC

Recommended dilution:

WB 1:500 – 1:2000 IHC 1:50 – 1:200

Recommended protocols: check protocols

Image descriptions:

Click or hover above images to see image description for Rabbit polyclonal PRKDC antibody.

Alternative names:

Check alternative names for the antibody

Expand

HYRC antibody, p350 antibody, DNAPK antibody, DNPK1 antibody, HYRC1 antibody, IMD26 antibody, XRCC7 antibody, DNA-PKcs antibody
DNA dependent protein kinase catalytic subunit antibody|DNA PK catalytic subunit antibody|DNA-dependent protein kinase catalytic subunit antibody|DNA-PK catalytic subunit antibody|DNA-PKcs antibody|DNAPK antibody|DNAPK catalytic subunit antibody|DNPK 1 antibody|DNPK1 antibody|Hyper radiosensitivity of murine scid mutation, complementing 1 antibody|HYRC 1 antibody|HYRC antibody|HYRC1 antibody|IMD26 antibody|p350 antibody|p460 antibody|PKRDC antibody|PRKDC antibody|PRKDC_HUMAN antibody|Protein Kinase DNA Activated Catalytic Polypeptide antibody|XRCC 7 antibody|XRCC7 antibody|Anti-DNA PKcs antibody [18-2] (ab1832)

SCBT cat No: sc-390698|sc-390849|sc-5282|sc-376207|

Name

Rabbit polyclonal PRKDC antibody

Catalogue No.

A7716

Reactivity

Human, Mouse

Immunogen

A synthetic peptide of human PRKDC

Host

Rabbit

Applications

WB, IHC

Recommended dilution

WB 1:500 – 1:2000
IHC 1:50 – 1:200

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

Predicted: 469kDa/Observed: Refer to figures

Formulation

PRKDC antibody was tube-contained.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Purification

PRKDC antibody was purified using affinity purification.

Storage

Store at -20 Celsius degree. Avoid freeze / thaw cycles.

Alternative antibody names

HYRC antibody, p350 antibody, DNAPK antibody, DNPK1 antibody, HYRC1 antibody, IMD26 antibody, XRCC7 antibody, DNA-PKcs antibody

Database links

Human UniProt/Swiss-Prot:P78527

Protein names

HYRC, p350, DNAPK, DNPK1, HYRC1, IMD26, XRCC7, DNA-PKcs

Protein function

Serine/threonine-protein kinase that acts as a molecular sensor for DNA damage. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break (DSB) repair and V(D)J recombination. Must be bound to DNA to express its catalytic properties. Promotes processing of hairpin DNA structures in V(D)J recombination by activation of the hairpin endonuclease artemis (DCLRE1C). The assembly of the DNA-PK complex at DNA ends is also required for the NHEJ ligation step. Required to protect and align broken ends of DNA. May also act as a scaffold protein to aid the localization of DNA repair proteins to the site of damage. Found at the ends of chromosomes, suggesting a further role in the maintenance of telomeric stability and the prevention of chromosomal end fusion. Also involved in modulation of transcription. Recognizes the substrate consensus sequence [ST]-Q. Phosphorylates ‘Ser-139’ of histone variant H2AX/H2AFX, thereby regulating DNA damage response mechanism. Phosphorylates DCLRE1C, c-Abl/ABL1, histone H1, HSPCA, c-jun/JUN, p53/TP53, PARP1, POU2F1, DHX9, SRF, XRCC1, XRCC1, XRCC4, XRCC5, XRCC6, WRN, MYC and RFA2. Can phosphorylate C1D not only in the presence of linear DNA but also in the presence of supercoiled DNA. Ability to phosphorylate p53/TP53 in the presence of supercoiled DNA is dependent on C1D. Contributes to the determination of the circadian period length by antagonizing phosphorylation of CRY1 ‘Ser-588’ and increasing CRY1 protein stability, most likely through an indirect machanism. Interacts with CRY1 and CRY2; negatively regulates CRY1 phosphorylation.

Protein sequence and domain

Belongs to the PI3/PI4-kinase family.; Contains 1 FAT domain.; Contains 1 FATC domain.; Contains 2 HEAT repeats.; Contains 1 PI3K/PI4K domain.; Contains 3 TPR repeats.

Protein post-translational modifications

Autophosphorylated on Ser-2056, Thr-2609, Thr-2638 and Thr-2647. Ser-2056 and Thr-2609 are DNA damage-inducible phosphorylation sites (inducible with ionizing radiation, IR) dephosphorylated by PPP5C. Autophosphorylation induces a conformational change that leads to remodeling of the DNA-PK complex, requisite for efficient end processing and DNA repair.; S-nitrosylated by GAPDH.; Polyubiquitinated by RNF144A, leading to proteasomal degradation.

Protein cellular localization

Nucleus . Nucleus, nucleolus .

Background

This gene encodes the catalytic subunit of the DNA-dependent protein kinase (DNA-PK). It functions with the Ku70/Ku80 heterodimer protein in DNA double strand break repair and recombination. The protein encoded is a member of the PI3/PI4-kinase family.

Research area

All research areas>Tumor Suppressor/Apoptosis>DNA-PK
(View all antibody categories related to Tumor Suppressor/Apoptosis)

Note

AntibodyPlus can customize PRKDC antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provides antibody trial sample for your own antibody validation.

Product type

Primary antibody

Supplier

ABclonal Inc.

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