Rabbit polyclonal PRKG1 antibody (A2565)

$69.00$259.00

Reactivity: Human,Mouse,Rat
Applications: WB,IHC
Conjugation: Unconjugated
Supplier: ABclonal Inc.

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Rabbit polyclonal PRKG1 antibody (A2565)

Supplier: ABclonal Inc.

Recommended applications: WB,IHC

Recommended dilution:

WB 1:500 – 1:2000 IHC 1:50 – 1:200

Recommended protocols: check protocols

Image descriptions:

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Alternative names:

Check alternative names for the antibody

Expand

PKG antibody, cGK antibody, AAT8 antibody, cGK1 antibody, cGKI antibody, cGK 1 antibody, PRKG1B antibody, PRKGR1B antibody, cGKI-BETA antibody, cGKI-alpha antibody
cGK 1 antibody|cGK1 antibody|cGKI alpha antibody|cGKI antibody|cGKI beta antibody|cGMP-dependent protein kinase 1 antibody|cGMP-dependent protein kinase I antibody|DKFZp686K042 antibody|FLJ36117 antibody|KGP1_HUMAN antibody|KGPB antibody|MGC71944 antibody|pkg antibody|PRKG1 antibody|PRKG1B antibody|PRKGR1A antibody|PRKGR1B antibody|Anti-cGKI antibody (ab90502)

SCBT cat No: sc-10338|sc-393987|sc-10335|sc-271765|sc-271766|sc-25429|sc-10343|sc-10342|sc-10341|

Name

Rabbit polyclonal PRKG1 antibody

Catalogue No.

A2565

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant protein of human PRKG1

Host

Rabbit

Applications

WB, IHC

Recommended dilution

WB 1:500 – 1:2000
IHC 1:50 – 1:200

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

Predicted: 76kDa/Observed: Refer to Figures

Formulation

PRKG1 antibody was tube-contained.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Purification

PRKG1 antibody was purified using affinity purification.

Storage

Store at -20 Celsius degree. Avoid freeze / thaw cycles.

Alternative antibody names

PKG antibody, cGK antibody, AAT8 antibody, cGK1 antibody, cGKI antibody, cGK 1 antibody, PRKG1B antibody, PRKGR1B antibody, cGKI-BETA antibody, cGKI-alpha antibody

Database links

Human UniProt/Swiss-Prot:Q13976

Protein names

PKG, cGK, AAT8, cGK1, cGKI, cGK 1, PRKG1B, PRKGR1B, cGKI-BETA, cGKI-alpha

Protein function

Serine/threonine protein kinase that acts as key mediator of the nitric oxide (NO)/cGMP signaling pathway. GMP binding activates PRKG1, which phosphorylates serines and threonines on many cellular proteins. Numerous protein targets for PRKG1 phosphorylation are implicated in modulating cellular calcium, but the contribution of each of these targets may vary substantially among cell types. Proteins that are phosphorylated by PRKG1 regulate platelet activation and adhesion, smooth muscle contraction, cardiac function, gene expression, feedback of the NO-signaling pathway, and other processes involved in several aspects of the CNS like axon guidance, hippocampal and cerebellar learning, circadian rhythm and nociception. Smooth muscle relaxation is mediated through lowering of intracellular free calcium, by desensitization of contractile proteins to calcium, and by decrease in the contractile state of smooth muscle or in platelet activation. Regulates intracellular calcium levels via several pathways: phosphorylates MRVI1/IRAG and inhibits IP3-induced Ca(2+) release from intracellular stores, phosphorylation of KCNMA1 (BKCa) channels decreases intracellular Ca(2+) levels, which leads to increased opening of this channel. PRKG1 phosphorylates the canonical transient receptor potential channel (TRPC) family which inactivates the associated inward calcium current. Another mode of action of NO/cGMP/PKGI signaling involves PKGI-mediated inactivation of the Ras homolog gene family member A (RhoA). Phosphorylation of RHOA by PRKG1 blocks the action of this protein in myriad processes: regulation of RHOA translocation; decreasing contraction; controlling vesicle trafficking, reduction of myosin light chain phosphorylation resulting in vasorelaxation. Activation of PRKG1 by NO signaling alters also gene expression in a number of tissues. In smooth muscle cells, increased cGMP and PRKG1 activity influence expression of smooth muscle-specific contractile proteins, levels of proteins in the NO/cGMP signaling pathway, down-regulation of the matrix proteins osteopontin and thrombospondin-1 to limit smooth muscle cell migration and phenotype. Regulates vasodilator-stimulated phosphoprotein (VASP) functions in platelets and smooth muscle.

Protein tissue specificity

Primarily expressed in lung and placenta.

Protein sequence and domain

Composed of an N-terminal leucine-zipper domain followed by an autoinhibitory domain, which mediate homodimer formation and inhibit kinase activity, respectively. Next, two cGMP-binding domains are followed by the catalytic domain at the C-terminus. Binding of cGMP to cGMP-binding domains results in a conformational change that activates kinase activity by removing the autoinhibitory domain from the catalytic cleft leaving the catalytic domain free to phosphorylate downstream substrates. Isoforms alpha and beta have identical cGMP-binding and catalytic domains but differ in their leucine zipper and autoinhibitory sequences and therefore differ in their dimerization substrates and kinase enzyme activity.; Heterotetramerization is mediated by the interaction between a coiled-coil of PRKG1 and the leucine/isoleucine zipper of PPP1R12A/MBS, the myosin-binding subunit of the myosin phosphatase.Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. cGMP subfamily.; Contains 1 AGC-kinase C-terminal domain.; Contains 2 cyclic nucleotide-binding domains.; Contains 1 protein kinase domain.

Protein post-translational modifications

Autophosphorylation increases kinase activity.; 65 kDa monomer is produced by proteolytic cleavage.

Protein cellular localization

Cytoplasm . Note: Colocalized with TRPC7 in the plasma membrane.

Background

Mammals have three different isoforms of cyclic GMP-dependent protein kinase (Ialpha, Ibeta, and II). These PRKG isoforms act as key mediators of the nitric oxide/cGMP signaling pathway and are important components of many signal transduction processes in diverse cell types. This PRKG1 gene on human chromosome 10 encodes the soluble Ialpha and Ibeta isoforms of PRKG by alternative transcript splicing. A separate gene on human chromosome 4, PRKG2, encodes the membrane-bound PRKG isoform II. The PRKG1 proteins play a central role in regulating cardiovascular and neuronal functions in addition to relaxing smooth muscle tone, preventing platelet aggregation, and modulating cell growth. This gene is most strongly expressed in all types of smooth muscle, platelets, cerebellar Purkinje cells, hippocampal neurons, and the lateral amygdala. Isoforms Ialpha and Ibeta have identical cGMP-binding and catalytic domains but differ in their leucine/isoleucine zipper and autoinhibitory sequences and therefore differ in their dimerization substrates and kinase enzyme activity.

Note

AntibodyPlus can customize PRKG1 antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provides antibody trial sample for your own antibody validation.

Product type

Primary antibody

Supplier

ABclonal Inc.

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