Rabbit polyclonal PRPF19 antibody (A6706)

$69.00$259.00

Reactivity: Human,Mouse,Rat
Applications: WB,IHC
Conjugation: Unconjugated
Supplier: ABclonal Inc.

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Rabbit polyclonal PRPF19 antibody (A6706)

Supplier: ABclonal Inc.

Recommended applications: WB,IHC

Recommended dilution:

WB 1:500 – 1:2000 IHC 1:100 – 1:200

Recommended protocols: check protocols

Image descriptions:

Click or hover above images to see image description for Rabbit polyclonal PRPF19 antibody.

Alternative names:

Check alternative names for the antibody

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PSO4 antibody, SNEV antibody, PRP19 antibody, UBOX4 antibody, hPSO4 antibody, NMP200 antibody
hPso4 antibody|pre mRNA processing factor 19 antibody|Pre-mRNA-processing factor 19 antibody|PRP19 antibody|PRP19/PSO4 homolog antibody|PRP19/PSO4 pre-mRNA processing factor 19 homolog (S. cerevisiae) antibody|PRP19_HUMAN antibody|PRPF19 antibody|PSO4 antibody|psoralen 4 antibody|Senescence evasion factor antibody|SNEV antibody|UBOX4 antibody|Anti-PRP19 antibody (ab27692)

SCBT cat No: sc-101236|sc-514338|

Name

Rabbit polyclonal PRPF19 antibody

Catalogue No.

A6706

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant protein of human PRPF19

Host

Rabbit

Applications

WB, IHC

Recommended dilution

WB 1:500 – 1:2000
IHC 1:100 – 1:200

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

Predicted: 55kDa/Observed: Refer to figures

Formulation

PRPF19 antibody was tube-contained.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Purification

PRPF19 antibody was purified using affinity purification.

Storage

Store at -20 Celsius degree. Avoid freeze / thaw cycles.

Alternative antibody names

PSO4 antibody, SNEV antibody, PRP19 antibody, UBOX4 antibody, hPSO4 antibody, NMP200 antibody

Database links

Human UniProt/Swiss-Prot:Q9UMS4

Protein names

PSO4, SNEV, PRP19, UBOX4, hPSO4, NMP200

Protein function

Ubiquitin-protein ligase which is a core component of several complexes mainly involved pre-mRNA splicing and DNA repair. Core component of the PRP19C/Prp19 complex/NTC/Nineteen complex which is part of the spliceosome and participates in its assembly, its remodeling and is required for its activity. During assembly of the spliceosome, mediates ‘Lys-63’-linked polyubiquitination of the U4 spliceosomal protein PRPF3. Ubiquitination of PRPF3 allows its recognition by the U5 component PRPF8 and stabilizes the U4/U5/U6 tri-snRNP spliceosomal complex . Recruited to RNA polymerase II C-terminal domain (CTD) and the pre-mRNA, it may also couple the transcriptional and spliceosomal machineries . The XAB2 complex, which contains PRPF19, is also involved in pre-mRNA splicing, transcription and transcription-coupled repair . Beside its role in pre-mRNA splicing PRPF19, as part of the PRP19-CDC5L complex, plays a role in the DNA damage response/DDR. It is recruited to the sites of DNA damage by the RPA complex where PRPF19 directly ubiquitinates RPA1 and RPA2. ‘Lys-63’-linked polyubiquitination of the RPA complex allows the recruitment of the ATR-ATRIP complex and the activation of ATR, a master regulator of the DNA damage response . May also play a role in DNA double-strand break (DSB) repair by recruiting the repair factor SETMAR to altered DNA . As part of the PSO4 complex may also be involved in the DNA interstrand cross-links/ICLs repair process . In addition, may also mediate ‘Lys-48’-linked polyubiquitination of substrates and play a role in proteasomal degradation . May play a role in the biogenesis of lipid droplets (By similarity). May play a role in neural differentiation possibly through its function as part of the spliceosome (By similarity).

Protein tissue specificity

Ubiquitous. Weakly expressed in senescent cells of different tissue origins. Highly expressed in tumor cell lines.

Protein sequence and domain

The 7 WD repeats are necessary and sufficient to support interaction with the RPA complex.Belongs to the WD repeat PRP19 family.; Contains 1 U-box domain.; Contains 7 WD repeats.

Protein cellular localization

Nucleus . Nucleus, nucleoplasm . Cytoplasm, cytoskeleton, spindle . Cytoplasm . Lipid droplet . Note: Nucleoplasmic in interphase cells. Irregularly distributed in anaphase cells. In prophase cells, uniformly distributed, but not associated with condensing chromosomes. Found in extrachromosomal regions in metaphase cells. Mainly localized to the mitotic spindle apparatus when chromosomes segregate during anaphase. When nuclei reform during late telophase, uniformly distributed in daughter cells and displays no preferred association with decondensing chromatin. Recruited on damaged DNA at sites of double-strand break.

Background

PSO4 is the human homolog of yeast Pso4, a gene essential for cell survival and DNA repair (Beck et al., 2008

Research area

All research areas>Transcription Regulators>Pre-mRNA-Processing
(View all antibody categories related to Transcription Regulators)

Note

AntibodyPlus can customize PRPF19 antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provides antibody trial sample for your own antibody validation.

Product type

Primary antibody

Supplier

ABclonal Inc.

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