Rabbit polyclonal PSAP antibody (A1819)

$69.00$259.00

Reactivity: Human,Mouse,Rat
Applications: WB,IHC,IF
Conjugation: Unconjugated
Supplier: ABclonal Inc.

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Rabbit polyclonal PSAP antibody (A1819)

Supplier: ABclonal Inc.

Recommended applications: WB,IHC,IF

Recommended dilution:

WB 1:500 – 1:2000 IHC 1:50 – 1:200 IF 1:50 – 1:200

Recommended protocols: check protocols

Image descriptions:

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Alternative names:

Check alternative names for the antibody

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FLJ00245 antibody, GLBA antibody, MGC110993 antibody, SAP1 antibody
A1 activator antibody|Cerebroside sulfate activator antibody|Co-beta-glucosidase antibody|Component C antibody|CSAct antibody|Dispersin antibody|GLBA antibody|Glucosylceramidase activator antibody|Proactivator polypeptide antibody|Proactivator polypeptide precursor antibody|Prosaposin (sphingolipid activator protein 1) antibody|prosaposin (variant Gaucher disease and variant metachromatic leukodystrophy) antibody|Prosaposin antibody|Protein A antibody|Protein C antibody|PSAP antibody|SAP-1 antibody|SAP-2 antibody|SAP_HUMAN antibody|SAP1 antibody|Saposin A antibody|Saposin B antibody|Saposin B Val antibody|Saposin C antibody|Saposin D antibody|Saposin-D antibody|Saposins antibody|Sgp1 antibody|Sphingolipid activator protein 1 antibody|Sphingolipid activator protein 2 antibody|Sulfated glycoprotein 1 antibody|Sulfatide/GM1 activator antibody|Anti-PSAP antibody (ab68466)

SCBT cat No: sc-32876|sc-374118|

Name

Rabbit polyclonal PSAP antibody

Catalogue No.

A1819

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant protein of human PSAP

Host

Rabbit

Applications

WB, IHC, IF

Recommended dilution

WB 1:500 – 1:2000
IHC 1:50 – 1:200
IF 1:50 – 1:200

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

Predicted: 58kDa/Observed: Refer to Figures

Formulation

PSAP antibody was tube-contained.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Purification

PSAP antibody was purified using affinity purification.

Storage

Store at -20 Celsius degree. Avoid freeze / thaw cycles.

Alternative antibody names

FLJ00245 antibody, GLBA antibody, MGC110993 antibody, SAP1 antibody

Database links

Human UniProt/Swiss-Prot:P07602

Protein names

FLJ00245, GLBA, MGC110993, SAP1

Protein function

Saposin-A and saposin-C stimulate the hydrolysis of glucosylceramide by beta-glucosylceramidase (EC 3.2.1.45) and galactosylceramide by beta-galactosylceramidase (EC 3.2.1.46). Saposin-C apparently acts by combining with the enzyme and acidic lipid to form an activated complex, rather than by solubilizing the substrate.; Saposin-B stimulates the hydrolysis of galacto-cerebroside sulfate by arylsulfatase A (EC 3.1.6.8), GM1 gangliosides by beta-galactosidase (EC 3.2.1.23) and globotriaosylceramide by alpha-galactosidase A (EC 3.2.1.22). Saposin-B forms a solubilizing complex with the substrates of the sphingolipid hydrolases.; Saposin-D is a specific sphingomyelin phosphodiesterase activator (EC 3.1.4.12).; Prosaposin: Behaves as a myelinotrophic and neurotrophic factor, these effects are mediated by its G-protein-coupled receptors, GPR37 and GPR37L1, undergoing ligand-mediated internalization followed by ERK phosphorylation signaling.; Saposins are specific low-molecular mass non-enzymic proteins, they participate in the lysosomal degradation of sphingolipids, which takes place by the sequential action of specific hydrolases.

Protein sequence and domain

Contains 2 saposin A-type domains.; Contains 4 saposin B-type domains.

Protein post-translational modifications

The lysosomal precursor is proteolytically processed to 4 small peptides, which are similar to each other and are sphingolipid hydrolase activator proteins.; N-linked glycans show a high degree of microheterogeneity.; The one residue extended Saposin-B-Val is only found in 5% of the chains.

Protein cellular localization

Lysosome .; Prosaposin: Secreted. Note: Secreted as a fully glycosylated 70 kDa protein composed of complex glycans.

Background

The PSAP gene encodes prosaposin, a precursor of four small nonenzymatic glycoproteins termed ‘sphingolipid activator proteins’ (SAPs) that assist in the lysosomal hydrolysis of sphingolipids. After proteolytic processing of the presaposin protein, these 4 released polypeptides are functional activators. Saposin A is encoded by residues 60 to 143 of PSAP, saposin B by 195 to 275, saposin C by 311 to 390, and saposin D by 405 to 487. They are four 12-14 kDa heatstable glycoproteins. Saposins A-D localize primarily to the lysosomal compartment where they facilitate the catabolism of glycosphingolipids with short oligosaccharide groups. Saposins A-D are required for the hydrolysis of certain sphingolipids by specific lysosomal hydrolases. (PMID: 2001789) Defects in PSAP are the cause of Gaucher disease, Tay-Sachs disease, and metachromatic leukodystrophy (PubMed: 2060627, PMID: 15773042). This PSAP antibody (10801-1-AP) is expected to recognize both saposin A and B.

Research area

All research areas>Synthesis and Degradation>saposin
(View all antibody categories related to Synthesis and Degradation)

Note

AntibodyPlus can customize PSAP antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provides antibody trial sample for your own antibody validation.

Product type

Primary antibody

Supplier

ABclonal Inc.

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