Rabbit polyclonal PSTPIP1 antibody (A7760)

$69.00$259.00

Reactivity: Human,Mouse,Rat
Applications: WB,IHC
Conjugation: Unconjugated
Supplier: ABclonal Inc.

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Rabbit polyclonal PSTPIP1 antibody (A7760)

Supplier: ABclonal Inc.

Recommended applications: WB,IHC

Recommended dilution:

WB 1:500 – 1:2000 IHC 1:50 – 1:200

Recommended protocols: check protocols

Image descriptions:

Click or hover above images to see image description for Rabbit polyclonal PSTPIP1 antibody.

Alternative names:

Check alternative names for the antibody

Expand

H-PIP antibody, PAPAS antibody, CD2BP1 antibody, PSTPIP antibody, CD2BP1L antibody, CD2BP1S antibody
CD2 antigen binding protein 1 antibody|CD2 binding protein 1 antibody|CD2 cytoplasmic tail binding protein antibody|CD2-binding protein 1 antibody|CD2BP1 antibody|CD2BP1L antibody|CD2BP1S antibody|H PIP antibody|H-PIP antibody|HPIP antibody|PAPAS antibody|PEST phosphatase interacting protein 1 antibody|PEST phosphatase-interacting protein 1 antibody|PPIP1_HUMAN antibody|Proline serine threonine phosphatase interacting protein 1 antibody|Proline-serine-threonine phosphatase-interacting protein 1 antibody|PSTPIP antibody|Pstpip1 antibody|Anti-PSTPIP1 antibody (ab88474)

SCBT cat No: sc-390727|sc-135221|sc-104630|

Name

Rabbit polyclonal PSTPIP1 antibody

Catalogue No.

A7760

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant protein of human PSTPIP1

Host

Rabbit

Applications

WB, IHC

Recommended dilution

WB 1:500 – 1:2000
IHC 1:50 – 1:200

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

Predicted: 48kDa/Observed: Refer to figures

Formulation

PSTPIP1 antibody was tube-contained.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Purification

PSTPIP1 antibody was purified using affinity purification.

Storage

Store at -20 Celsius degree. Avoid freeze / thaw cycles.

Alternative antibody names

H-PIP antibody, PAPAS antibody, CD2BP1 antibody, PSTPIP antibody, CD2BP1L antibody, CD2BP1S antibody

Database links

Human UniProt/Swiss-Prot:O43586

Protein names

H-PIP, PAPAS, CD2BP1, PSTPIP, CD2BP1L, CD2BP1S

Protein function

Involved in regulation of the actin cytoskeleton. May regulate WAS actin-bundling activity. Bridges the interaction between ABL1 and PTPN18 leading to ABL1 dephosphorylation. May play a role as a scaffold protein between PTPN12 and WAS and allow PTPN12 to dephosphorylate WAS. Has the potential to physically couple CD2 and CD2AP to WAS. Acts downstream of CD2 and CD2AP to recruit WAS to the T-cell:APC contact site so as to promote the actin polymerization required for synapse induction during T-cell activation (By similarity). Down-regulates CD2-stimulated adhesion through the coupling of PTPN12 to CD2. Also has a role in innate immunity and the inflammatory response. Recruited to inflammasomes by MEFV. Induces formation of pyroptosomes, large supramolecular structures composed of oligomerized PYCARD dimers which form prior to inflammatory apoptosis. Binding to MEFV allows MEFV to bind to PYCARD and facilitates pyroptosome formation. Regulates endocytosis and cell migration in neutrophils.

Protein tissue specificity

Highly expressed in the peripheral blood leukocytes, granulocytes and monocytes, namely in T-cells and natural killer cells, and in spleen. Weakly expressed in the thymus, small intestine, lung and placenta.

Protein sequence and domain

The F-BAR domain is important for filament formation. The SH3 domain is not required for filament formation or localization to the uropod.Contains 1 F-BAR domain.; Contains 1 SH3 domain.

Protein post-translational modifications

Dephosphorylated on Tyr-345 by PTPN18, this event negatively regulates the association of PSTPIP1 with SH2 domain-containing proteins as tyrosine kinase. Phosphorylation of Tyr-345 is probably required for subsequent phosphorylation at other tyrosine residues. Phosphorylation is induced by activation of the EGFR and PDGFR in a ABL1 dependent manner. The phosphorylation regulates the interaction with WAS and with MEFV (By similarity).

Protein cellular localization

Cytoplasm . Cell membrane ; Peripheral membrane protein . Cell projection, uropodium . Cytoplasm, cytoskeleton . Cytoplasm, perinuclear region . Cell projection, lamellipodium . Cleavage furrow . Note: Mainly cytoplasmic in T-cells . Colocalizes in cluster with CD2 near the cell surface membrane in activated T-cells . In monocytes, forms a branched filamentous network in the cytoplasm . In transfected cells, forms relatively straight filaments radiating out from the nucleus . Filament formation requires an intact tubulin cytoskeleton . In migrating neutrophils, colocalizes with PIP5K1C and DNM2 to the trailing edge of the uropod in a actin-dependent manner . Colocalized with PTPN12 in the cytoplasm and the perinuclear region. During interphase, colocalizes with F-actin in the cortical cytoskeleton, lamellipodia, and stress fibers. In dividing cells, colocalizes with the F-actin rich cytokinetic cleavage furrow. Colocalized with CD2AP and WAS in the actin cytoskeleton within the cytoplasm. Colocalized with CD2, CD2AP and WAS at the site of T-cell:APC contact (By similarity).

Background

The protein encoded by this gene binds to the cytoplasmic tail of CD2, an effector of T cell activation and adhesion, negatively affecting CD2-triggered T cell activation. The encoded protein appears to be a scaffold protein and a regulator of the actin cytoskeleton. It has also been shown to bind ABL1, PTPN18, WAS, CD2AP, and PTPN12. Mutations in this gene are a cause of PAPA syndrome.

Note

AntibodyPlus can customize PSTPIP1 antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provides antibody trial sample for your own antibody validation.

Product type

Primary antibody

Supplier

ABclonal Inc.

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