Rabbit polyclonal PTCH1 antibody (A0826)

$69.00$259.00

Reactivity: Human,Mouse
Applications: WB
Conjugation: Unconjugated
Supplier: ABclonal Inc.

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Rabbit polyclonal PTCH1 antibody (A0826)

Supplier: ABclonal Inc.

Recommended applications: WB

Recommended dilution:

WB 1:500 – 1:1000

Recommended protocols: check protocols

Image descriptions:

Click or hover above images to see image description for Rabbit polyclonal PTCH1 antibody.

Alternative names:

Check alternative names for the antibody

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BCNS antibody, FLJ26746 antibody, FLJ42602 antibody, HPE7 antibody, NBCCS antibody, PTC antibody, PTC1 antibody, PTCH antibody, PTCH11 antibody
A230106A15Rik antibody|BCNS antibody|FLJ26746 antibody|FLJ42602 antibody|Holoprosencephaly 7 antibody|HPE7 antibody|mes antibody|NBCCS antibody|OTTHUMP00000021709 antibody|OTTHUMP00000021710 antibody|Patched antibody|Patched (Drosophila) homolog antibody|Patched 1 antibody|Patched homolog (Drosophila) antibody|Patched homolog 1 (Drosophila) antibody|Patched homolog 1 antibody|Patched protein homolog 1 antibody|Protein patched homolog 1 antibody|PTC antibody|PTC1 antibody|PTC1_HUMAN antibody|PTCH antibody|PTCH protein +12b antibody|PTCH protein +4′ antibody|PTCH protein -10 antibody|PTCH protein antibody|ptch1 antibody|PTCH1 protein antibody|PTCH11 antibody|Ptch2 antibody|Anti-Patched / PTCH1 antibody (ab53715)

SCBT cat No: sc-293416|sc-6147|sc-6149|sc-9016|sc-34122|

Name

Rabbit polyclonal PTCH1 antibody

Catalogue No.

A0826

Reactivity

Human, Mouse

Immunogen

Recombinant protein of human PTCH1

Host

Rabbit

Applications

WB

Recommended dilution

WB 1:500 – 1:1000

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

Predicted: 161kDa/Observed: Refer to Figures

Formulation

PTCH1 antibody was tube-contained.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Purification

PTCH1 antibody was purified using affinity purification.

Storage

Store at -20 Celsius degree. Avoid freeze / thaw cycles.

Alternative antibody names

BCNS antibody, FLJ26746 antibody, FLJ42602 antibody, HPE7 antibody, NBCCS antibody, PTC antibody, PTC1 antibody, PTCH antibody, PTCH11 antibody

Database links

Human UniProt/Swiss-Prot:Q13635

Protein names

BCNS, FLJ26746, FLJ42602, HPE7, NBCCS, PTC, PTC1, PTCH, PTCH11

Protein function

Acts as a receptor for sonic hedgehog (SHH), indian hedgehog (IHH) and desert hedgehog (DHH). Associates with the smoothened protein (SMO) to transduce the hedgehog’s proteins signal. Seems to have a tumor suppressor function, as inactivation of this protein is probably a necessary, if not sufficient step for tumorigenesis.

Protein tissue specificity

In the adult, expressed in brain, lung, liver, heart, placenta, skeletal muscle, pancreas and kidney. Expressed in tumor cells but not in normal skin.

Protein sequence and domain

Belongs to the patched family.; Contains 1 SSD (sterol-sensing) domain.

Protein post-translational modifications

Glycosylation is necessary for SHH binding.; In the absence of Hh ligands, ubiquitination by ITCH at Lys-1426 promotes endocytosis and both proteasomal and lysosomal degradation.

Protein cellular localization

Membrane; Multi-pass membrane protein.

Background

Patched1 and 2 (PTCH1 and PTCH2) are twelve-pass transmembrane proteins that function as the receiving receptors for members of the Hedgehog family of proteins (1-4). In the absence of Hedgehog proteins, PTCH suppresses the otherwise constitutively active signaling receptor Smoothened (Smo) so that the Hedgehog signaling pathway is in the off state (5,6). Deactivating mutations that impair the ability of PTCH1 to suppress Smo are frequently found in patients with nevoid basal cell carcinoma syndrome (7,8). PTCH proteins have a sterol-sensing domain (SSD) also found in several proteins that function in cholesterol homeostasis, such as HMGCR (3-hydroxy-3-methylglutaryl coenzyme A-reductase) and SCAP (sterol regulatory element-binding protein-cleavage activating protein). However, the role of the SSD in Patched proteins is not clear (9,10).PTCH1 itself is a target of Hedgehog signaling (11), with elevated PTCH1 expression as a surrogate marker for Hedgehog pathway activation (12-14).

Research area

All research areas>Membrane Receptors>Patched
(View all antibody categories related to Membrane Receptors)

Note

AntibodyPlus can customize PTCH1 antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provides antibody trial sample for your own antibody validation.

Product type

Primary antibody

Supplier

ABclonal Inc.

Reviews


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Antibody customer reviews & validation data - 188

Validation data ID: 188

Submission date: 2017-05-04

View Details

Result figure:
  • Result figure
  • Result figure

Figure description:

1776 - 2 min exp
1777 - 15 min exp
THe bightest band is not in the right MW. Its around 90KDa.
However, there is a band around 160 which is the 2nd brightest.

Product name

PTCH1

Product catalog No.

A0826

Product type

Primary antibody

Supplier

St John's Laboratory Ltd

Application type

Western Blot

Antibody specificity rating

Good

Antibody overall rating

Good

Your comments

Multiple bands observed; however, there was one in the right MW range

Detailed protocol

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