Rabbit polyclonal PTTG1 antibody (A8307)

$69.00$259.00

Reactivity: Human,Mouse,Rat
Applications: WB,IHC
Conjugation: Unconjugated
Supplier: ABclonal Inc.

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Rabbit polyclonal PTTG1 antibody (A8307)

Supplier: ABclonal Inc.

Recommended applications: WB,IHC

Recommended dilution:

WB 1:500 – 1:2000 IHC 1:100 – 1:200

Recommended protocols: check protocols

Image descriptions:

Click or hover above images to see image description for Rabbit polyclonal PTTG1 antibody.

Alternative names:

Check alternative names for the antibody

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EAP1 antibody, PTTG antibody, HPTTG antibody, TUTR1 antibody
AW555095 antibody|C87862 antibody|Cut2 antibody|EAP 1 antibody|EAP1 antibody|ESP1 associated protein 1 antibody|Esp1-associated protein antibody|hPTTG antibody|MGC126883 antibody|MGC138276 antibody|Pds1 antibody|Pituitary tumor transforming 1 antibody|Pituitary tumor transforming protein 1 antibody|Pituitary tumor-transforming 1, isoform CRA_a antibody|Pituitary tumor-transforming 1, isoform CRA_b antibody|Pituitary tumor-transforming gene 1 antibody|Pituitary tumor-transforming gene 1 protein antibody|PTTG 1 antibody|PTTG antibody|PTTG1 antibody|PTTG1 protein antibody|PTTG1_HUMAN antibody|Pttg3 antibody|Securin antibody|Tumor transforming 1 antibody|Tumor transforming protein 1 antibody|Tumor-transforming protein 1 antibody|TUTR 1 antibody|TUTR1 antibody|Anti-Securin antibody [DCS-280] (ab3305)

SCBT cat No: sc-71947|sc-5839|sc-398471|sc-56207|sc-376454|sc-22772|sc-514984|

Name

Rabbit polyclonal PTTG1 antibody

Catalogue No.

A8307

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant protein of human PTTG1

Host

Rabbit

Applications

WB, IHC

Recommended dilution

WB 1:500 – 1:2000
IHC 1:100 – 1:200

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

Predicted: 22kDa/Observed: Refer to figures

Formulation

PTTG1 antibody was tube-contained.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Purification

PTTG1 antibody was purified using affinity purification.

Storage

Store at -20 Celsius degree. Avoid freeze / thaw cycles.

Alternative antibody names

EAP1 antibody, PTTG antibody, HPTTG antibody, TUTR1 antibody

Database links

Human UniProt/Swiss-Prot:O95997

Protein names

EAP1, PTTG, HPTTG, TUTR1

Protein function

Regulatory protein, which plays a central role in chromosome stability, in the p53/TP53 pathway, and DNA repair. Probably acts by blocking the action of key proteins. During the mitosis, it blocks Separase/ESPL1 function, preventing the proteolysis of the cohesin complex and the subsequent segregation of the chromosomes. At the onset of anaphase, it is ubiquitinated, conducting to its destruction and to the liberation of ESPL1. Its function is however not limited to a blocking activity, since it is required to activate ESPL1. Negatively regulates the transcriptional activity and related apoptosis activity of TP53. The negative regulation of TP53 may explain the strong transforming capability of the protein when it is overexpressed. May also play a role in DNA repair via its interaction with Ku, possibly by connecting DNA damage-response pathways with sister chromatid separation.

Protein tissue specificity

Expressed at low level in most tissues, except in adult testis, where it is highly expressed. Overexpressed in many patients suffering from pituitary adenomas, primary epithelial neoplasias, and esophageal cancer.

Protein sequence and domain

The N-terminal destruction box (D-box) acts as a recognition signal for degradation via the ubiquitin-proteasome pathway.; The TEK-boxes are required for ‘Lys-11’-linked ubiquitination and facilitate the transfer of the first ubiquitin and ubiquitin chain nucleation. TEK-boxes may direct a catalytically competent orientation of the UBE2C/UBCH10-ubiquitin thioester with the acceptor lysine residue.Belongs to the securin family.

Protein post-translational modifications

Phosphorylated at Ser-165 by CDK1 during mitosis.; Phosphorylated in vitro by ds-DNA kinase.; Ubiquitinated through ‘Lys-11’ linkage of ubiquitin moieties by the anaphase promoting complex (APC) at the onset of anaphase, conducting to its degradation. ‘Lys-11’-linked ubiquitination is mediated by the E2 ligase UBE2C/UBCH10.

Protein cellular localization

Cytoplasm. Nucleus.

Background

The encoded protein is a homolog of yeast securin proteins, which prevent separins from promoting sister chromatid separation. It is an anaphase-promoting complex (APC) substrate that associates with a separin until activation of the APC. The gene product has transforming activity in vitro and tumorigenic activity in vivo, and the gene is highly expressed in various tumors. The gene product contains 2 PXXP motifs, which are required for its transforming and tumorigenic activities, as well as for its stimulation of basic fibroblast growth factor expression. It also contains a destruction box (D box) that is required for its degradation by the APC. The acidic C-terminal region of the encoded protein can act as a transactivation domain. The gene product is mainly a cytosolic protein, although it partially localizes in the nucleus. Three transcript variants encoding the same protein have been found for this gene.

Note

AntibodyPlus can customize PTTG1 antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provides antibody trial sample for your own antibody validation.

Product type

Primary antibody

Supplier

ABclonal Inc.

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