Rabbit polyclonal PUF60 antibody (A6709)

$69.00$259.00

Reactivity: Human,Mouse,Rat
Applications: WB,IHC,IF
Conjugation: Unconjugated
Supplier: ABclonal Inc.

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Rabbit polyclonal PUF60 antibody (A6709)

Supplier: ABclonal Inc.

Recommended applications: WB,IHC,IF

Recommended dilution:

WB 1:500 – 1:2000 IHC 1:50 – 1:200 IF 1:50 – 1:200

Recommended protocols: check protocols

Image descriptions:

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Alternative names:

Check alternative names for the antibody

Expand

FIR antibody, VRJS antibody, RoBPI antibody, SIAHBP1 antibody
60 kDa poly(U)-binding-splicing factor antibody|FBP-interacting repressor antibody|FIR antibody|FLJ31379 antibody|FUSE-binding protein-interacting repressor antibody|Poly U binding splicing factor 60KDa antibody|Poly(U)-binding-splicing factor PUF60 antibody|poly-U binding splicing factor PUF60 antibody|PUF60 antibody|PUF60_HUMAN antibody|pyrimidine tract binding splicing factor antibody|Ro ribonucleoprotein-binding protein 1 antibody|Ro-binding protein 1 antibody|RoBP1 antibody|RoBPI antibody|siah binding protein 1 antibody|Siah-binding protein 1 antibody|Siah-BP1 antibody|SIAHBP1 antibody|Anti-PUF60 antibody [GT677] (ab184538)

SCBT cat No: sc-133583|

Name

Rabbit polyclonal PUF60 antibody

Catalogue No.

A6709

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant protein of human PUF60

Host

Rabbit

Applications

WB, IHC, IF

Recommended dilution

WB 1:500 – 1:2000
IHC 1:50 – 1:200
IF 1:50 – 1:200

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

Predicted: 59kDa/Observed: Refer to figures

Formulation

PUF60 antibody was tube-contained.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Purification

PUF60 antibody was purified using affinity purification.

Storage

Store at -20 Celsius degree. Avoid freeze / thaw cycles.

Alternative antibody names

FIR antibody, VRJS antibody, RoBPI antibody, SIAHBP1 antibody

Database links

Human UniProt/Swiss-Prot:Q9UHX1

Protein names

FIR, VRJS, RoBPI, SIAHBP1

Protein function

DNA- and RNA-binding protein, involved in several nuclear processes such as pre-mRNA splicing, apoptosis and transcription regulation. In association with FUBP1 regulates MYC transcription at the P2 promoter through the core-TFIIH basal transcription factor. Acts as a transcriptional repressor through the core-TFIIH basal transcription factor. Represses FUBP1-induced transcriptional activation but not basal transcription. Decreases ERCC3 helicase activity. Does not repress TFIIH-mediated transcription in xeroderma pigmentosum complementation group B (XPB) cells. Is also involved in pre-mRNA splicing. Promotes splicing of an intron with weak 3′-splice site and pyrimidine tract in a cooperative manner with U2AF2. Involved in apoptosis induction when overexpressed in HeLa cells. Isoform 6 failed to repress MYC transcription and inhibited FIR-induced apoptosis in colorectal cancer. Isoform 6 may contribute to tumor progression by enabling increased MYC expression and greater resistance to apoptosis in tumors than in normal cells. Modulates alternative splicing of several mRNAs. Binds to relaxed DNA of active promoter regions. Binds to the pyrimidine tract and 3′-splice site regions of pre-mRNA; binding is enhanced in presence of U2AF2. Binds to Y5 RNA in association with TROVE2. Binds to poly(U) RNA.

Protein tissue specificity

Isoform 2 is expressed in colonic epithelium and colorectal epithelium cancer (at protein level). Isoform 6 is expressed in colorectal epithelial cancer but below detection level in colonic epithelium. Expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney, pancreas, spleen, thymus, prostate, testis, ovary, small intestine, colon and peripheral blood leukocytes.

Protein sequence and domain

The third RNA recognition motif, called PUMP domain, is atypical and may rather mediate homodimerization and/or protein-protein interactions.Belongs to the RRM half pint family.; Contains 3 RRM (RNA recognition motif) domains.

Protein cellular localization

Nucleus . Note: Colocalizes partially with TROVE2.

Background

This gene encodes a nucleic acid-binding protein that plays a role in a variety of nuclear processes, including pre-mRNA splicing and transcriptional regulation. The encoded protein forms a complex with the far upstream DNA element (FUSE) and FUSE-binding protein at the myelocytomatosis oncogene (MYC) promoter. This complex represses MYC transcription through the core-TFIIH basal transcription factor. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.

Research area

All research areas>Transcription Regulators>FIR
(View all antibody categories related to Transcription Regulators)

Note

AntibodyPlus can customize PUF60 antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provides antibody trial sample for your own antibody validation.

Product type

Primary antibody

Supplier

ABclonal Inc.

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