Rabbit polyclonal PYCARD antibody (A1170)

$69.00$259.00

Reactivity: Human,Mouse
Applications: WB,IF
Conjugation: Unconjugated
Supplier: ABclonal Inc.

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Rabbit polyclonal PYCARD antibody (A1170)

Supplier: ABclonal Inc.

Recommended applications: WB,IF

Recommended dilution:

WB 1:500 – 1:2000 IF 1:50 – 1:200

Recommended protocols: check protocols

Image descriptions:

Click or hover above images to see image description for Rabbit polyclonal PYCARD antibody.

Alternative names:

Check alternative names for the antibody

Expand

PYCARD antibody,ASC antibody,CARD5 antibody,MGC10332 antibody,TMS antibody,TMS-1 antibody,TMS1 antibody
Apoptosis associated speck like protein containing a CARD antibody|Apoptosis-associated speck-like protein containing a CARD antibody|ASC antibody|ASC_HUMAN antibody|CARD 5 antibody|CARD5 antibody|Caspase recruitment domain containing protein 5 antibody|Caspase recruitment domain protein 5 antibody|Caspase recruitment domain-containing protein 5 antibody|hASC antibody|MGC10332 antibody|PYCARD antibody|PYD and CARD domain containing antibody|PYD and CARD domain containing protein antibody|PYD and CARD domain-containing protein antibody|Target of methylation induced silencing 1 antibody|Target of methylation-induced silencing 1 antibody|TMS 1 antibody|TMS antibody|TMS1 antibody|Anti-TMS1 antibody [2A7] (ab128097)

SCBT cat No: sc-514414|sc-33958|sc-514559|sc-271054|sc-30153|sc-22514|

Name

Rabbit polyclonal PYCARD antibody

Catalogue No.

A1170

Reactivity

Human, Mouse

Immunogen

Recombinant protein of human PYCARD

Host

Rabbit

Applications

WB, IF

Recommended dilution

WB 1:500 – 1:2000
IF 1:50 – 1:200

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

Predicted: 25kDa/Observed: Refer to Figures

Formulation

PYCARD antibody was tube-contained.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Purification

PYCARD antibody was purified using affinity purification.

Storage

Store at -20 Celsius degree. Avoid freeze / thaw cycles.

Alternative antibody names

PYCARD antibody,ASC antibody,CARD5 antibody,MGC10332 antibody,TMS antibody,TMS-1 antibody,TMS1 antibody

Database links

Human UniProt/Swiss-Prot:Q9ULZ3

Protein names

PYCARD,ASC,CARD5,MGC10332,TMS,TMS-1,TMS1

Protein function

Functions as key mediator in apoptosis and inflammation. Promotes caspase-mediated apoptosis involving predominantly caspase-8 and also caspase-9 in a probable cell type-specific manner. Involved in activation of the mitochondrial apoptotic pathway, promotes caspase-8-dependent proteolytic maturation of BID independently of FADD in certain cell types and also mediates mitochondrial translocation of BAX and activates BAX-dependent apoptosis coupled to activation of caspase-9, -2 and -3. Involved in macrophage pyroptosis, a caspase-1-dependent inflammatory form of cell death and is the major constituent of the ASC pyroptosome which forms upon potassium depletion and rapidly recruits and activates caspase-1. In innate immune response believed to act as an integral adapter in the assembly of the inflammasome which activates caspase-1 leading to processing and secretion of proinflammatory cytokines. The function as activating adapter in different types of inflammasomes is mediated by the pyrin and CARD domains and their homotypic interactions. Required for recruitment of caspase-1 to inflammasomes containing certain pattern recognition receptors, such as NLRP2, NLRP3, AIM2 and probably IFI16. In the NLRP1 and NLRC4 inflammasomes seems not be required but facilitates the processing of procaspase-1. In cooperation with NOD2 involved in an inflammasome activated by bacterial muramyl dipeptide leading to caspase-1 activation. May be involved in DDX58-triggered proinflammatory responses and inflammasome activation. Isoform 2 may have a regulating effect on the function as inflammasome adapter. Isoform 3 seems to inhibit inflammasome-mediated maturation of interleukin-1 beta. In collaboration with AIM2 which detects cytosolic double-stranded DNA may also be involved in a caspase-1-independent cell death that involves caspase-8. In adaptive immunity may be involved in maturation of dendritic cells to stimulate T-cell immunity and in cytoskeletal rearrangements coupled to chemotaxis and antigen uptake may be involved in post-transcriptional regulation of the guanine nucleotide exchange factor DOCK2; the latter function is proposed to involve the nuclear form. Also involved in transcriptional activation of cytokines and chemokines independent of the inflammasome; this function may involve AP-1, NF-kappa-B, MAPK and caspase-8 signaling pathways. For regulation of NF-kappa-B activating and inhibiting functions have been reported. Modulates NF-kappa-B induction at the level of the IKK complex by inhibiting kinase activity of CHUK and IKBK. Proposed to compete with RIPK2 for association with CASP1 thereby down-regulating CASP1-mediated RIPK2-dependent NF-kappa-B activation and activating interleukin-1 beta processing.

Protein tissue specificity

Widely expressed at low levels. Detected in peripheral blood leukocytes, lung, small intestine, spleen, thymus, colon and at lower levels in placenta, liver and kidney. Very low expression in skeletal muscle, heart and brain. Detected in the leukemia cell lines HL-60 and U-937, but not in Jurkat T-cell lymphoma and Daudi Burkitt’s lymphoma. Detected in the melanoma cell line WM35, but not in WM793. Not detected in HeLa cervical carcinoma cells and MOLT-4 lymphocytic leukemia cells.

Protein sequence and domain

The CARD domain mediates interaction with CASP1 and NLRC4 .; The pyrin domain mediates homotypic interactions with pyrin domains of proteins such as of NLRP3, PYDC1, PYDC2 and AIM2.Contains 1 CARD domain.; Contains 1 pyrin domain.

Protein post-translational modifications

Phosphorylated.

Protein cellular localization

Cytoplasm. Endoplasmic reticulum. Mitochondrion. Nucleus. Note: Upstream of caspase activation, a redistribution from the cytoplasm to the aggregates occurs. These appear as hollow, perinuclear spherical, ball-like structures. Upon NLRP3 inflammasome activation redistributes to the perinuclear space localizing to endoplasmic reticulum and mitochondria. Localized primarily to the nucleus in resting monocytes/macrophages and rapidly redistributed to the cytoplasm upon pathogen infection. Localized to large cytoplasmic aggregate appearing as a speck containing AIM2, PYCARD, CASP8 and bacterial DNA after infection with Francisella tularensis (By similarity).

Background

TMS1 (target of methylation-induced silencing)/ASC (apoptosis-associated speck-like protein containing a CARD), also referred to as PYCARD and CARD5, is a 22-kDa pro-apoptotic protein containing an N-terminal pyrin domain (PYD) and a C-terminal caspase recruitment domain (CARD) (1-2). The TMS1 gene was originally found to be aberrantly methylated and silenced in breast cancer cells (2), and has since been found to be silenced in a number of other cancers, including ovarian cancer (3), glioblastoma (4), melanoma (5), gastric cancer (6), lung cancer (7), and prostate cancer (8). Expression of TMS1 can be induced by pro-apoptotic/inflammatory stimuli (9). During apoptosis TMS1 is re-distributed from the cytosol to the mitochondria and associates with mitochondrial Bax to trigger cytochrome c release and subsequent apoptosis (10). TMS1 has also been found to be a critical component of inflammatory signaling where it associates with and activates caspase-1 in response to pro-inflammatory signals (11).

Research area

All research areas>Tumor Suppressor/Apoptosis>ASC
(View all antibody categories related to Tumor Suppressor/Apoptosis)

Note

AntibodyPlus can customize PYCARD antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provides antibody trial sample for your own antibody validation.

Product type

Primary antibody

Supplier

ABclonal Inc.

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