Rabbit polyclonal RAD17 antibody (A5359)

$69.00$259.00

Reactivity: Human,Mouse,Rat
Applications: WB,IHC,IF
Conjugation: Unconjugated
Supplier: ABclonal Inc.

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Rabbit polyclonal RAD17 antibody (A5359)

Supplier: ABclonal Inc.

Recommended applications: WB,IHC,IF

Recommended dilution:

WB 1:500 – 1:2000 IHC 1:50 – 1:200 IF 1:10 – 1:100

Recommended protocols: check protocols

Image descriptions:

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Alternative names:

Check alternative names for the antibody

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CCYC antibody, R24L antibody, RAD24 antibody, HRAD17 antibody, RAD17SP antibody
CCYC antibody|Cell cycle checkpoint protein antibody|Cell cycle checkpoint protein RAD17 antibody|FLJ41520 antibody|HRAD 17 antibody|hRad17 antibody|R24L antibody|Rad 17 antibody|Rad 24 antibody|RAD1 (S. pombe) homolog antibody|RAD1 homolog antibody|RAD17 antibody|RAD17 homolog (S. pombe) antibody|RAD17 homolog antibody|Rad17 like protein antibody|RAD17, S. pombe, homolog of antibody|RAD17_HUMAN antibody|RAD17Sp antibody|Rad24 antibody|Rad24, mouse, homolog of antibody|Rad24, S. cerevisiae, homolog of antibody|RF C activator 1 homolog antibody|RF C/activator 1 homolog antibody|RF-C/activator 1 homolog antibody|Phospho anti-Rad17 (S645) antibody (ab3620)

SCBT cat No: sc-32842|sc-32843|sc-81513|sc-271929|sc-271928|

Name

Rabbit polyclonal RAD17 antibody

Catalogue No.

A5359

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant protein of human RAD17

Host

Rabbit

Applications

WB, IHC, IF

Recommended dilution

WB 1:500 – 1:2000
IHC 1:50 – 1:200
IF 1:10 – 1:100

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

Predicted: 77kDa/Observed: Refer to Figures

Formulation

RAD17 antibody was tube-contained.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Purification

RAD17 antibody was purified using affinity purification.

Storage

Store at -20 Celsius degree. Avoid freeze / thaw cycles.

Alternative antibody names

CCYC antibody, R24L antibody, RAD24 antibody, HRAD17 antibody, RAD17SP antibody

Database links

Human UniProt/Swiss-Prot:O75943

Protein names

CCYC, R24L, RAD24, HRAD17, RAD17SP

Protein function

Essential for sustained cell growth, maintenance of chromosomal stability, and ATR-dependent checkpoint activation upon DNA damage. Has a weak ATPase activity required for binding to chromatin. Participates in the recruitment of the RAD1-RAD9-HUS1 complex and RHNO1 onto chromatin, and in CHEK1 activation. May also serve as a sensor of DNA replication progression, and may be involved in homologous recombination.

Protein tissue specificity

Overexpressed in various cancer cell lines and in colon carcinoma (at protein level). Isoform 2 and isoform 3 are the most abundant isoforms in non irradiated cells (at protein level). Ubiquitous at low levels. Highly expressed in testis, where it is expressed within the germinal epithelium of the seminiferous tubuli. Weakly expressed in seminomas (testicular tumors).

Protein sequence and domain

Belongs to the rad17/RAD24 family.

Protein post-translational modifications

Phosphorylated. Phosphorylation on Ser-646 and Ser-656 is cell cycle-regulated, enhanced by genotoxic stress, and required for activation of checkpoint signaling. Phosphorylation is mediated by ATR upon UV or replication arrest, whereas it may be mediated both by ATR and ATM upon ionizing radiation. Phosphorylation on both sites is required for interaction with RAD1 but dispensable for interaction with RFC3 or RFC4.

Protein cellular localization

Nucleus . Note: Phosphorylated form redistributes to discrete nuclear foci upon DNA damage.

Background

The protein encoded by this gene is highly similar to the gene product of Schizosaccharomyces pombe rad17, a cell cycle checkpoint gene required for cell cycle arrest and DNA damage repair in response to DNA damage. This protein shares strong similarity with DNA replication factor C (RFC), and can form a complex with RFCs. This protein binds to chromatin prior to DNA damage and is phosphorylated by the checkpoint kinase ATR following damage. This protein recruits the RAD1-RAD9-HUS1 checkpoint protein complex onto chromatin after DNA damage, which may be required for its phosphorylation. The phosphorylation of this protein is required for the DNA-damage-induced cell cycle G2 arrest, and is thought to be a critical early event during checkpoint signaling in DNA-damaged cells. Multiple alternatively spliced transcript variants of this gene, which encode four distinct protein isoforms, have been reported. Two pseudogenes, located on chromosomes 7 and 13, have been identified.

Research area

All research areas>Tumor Suppressor/Apoptosis>Rad
(View all antibody categories related to Tumor Suppressor/Apoptosis)

Note

AntibodyPlus can customize RAD17 antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provides antibody trial sample for your own antibody validation.

Product type

Primary antibody

Supplier

ABclonal Inc.

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