Rabbit polyclonal RAD50 antibody (A3078)

$69.00$259.00

Reactivity: Human,Mouse
Applications: WB,IHC,IF
Conjugation: Unconjugated
Supplier: ABclonal Inc.

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Rabbit polyclonal RAD50 antibody (A3078)

Supplier: ABclonal Inc.

Recommended applications: WB,IHC,IF

Recommended dilution:

WB 1:500 – 1:2000 IHC 1:50 – 1:200 IF 1:50 – 1:200

Recommended protocols: check protocols

Image descriptions:

Click or hover above images to see image description for Rabbit polyclonal RAD50 antibody.

Alternative names:

Check alternative names for the antibody

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NBSLD antibody, RAD502 antibody, hRad50 antibody
DNA repair protein RAD50 antibody|hRAD50 antibody|hsRAD50 antibody|Mrell antibody|NBSLD antibody|Rad 50 antibody|RAD50 2 antibody|rad50 antibody|RAD50 homolog (S cerevisiae) antibody|RAD50 homolog antibody|RAD50 PEN antibody|RAD50, S. cerevisiae, homolog of antibody|RAD50_HUMAN antibody|RAD502 antibody|Rad50l antibody|Truncated RAD50 protein antibody|Anti-Rad50 antibody [13B3/2C6] (ab89)

SCBT cat No: sc-56209|sc-18291|sc-74460|sc-20155|

Name

Rabbit polyclonal RAD50 antibody

Catalogue No.

A3078

Reactivity

Human, Mouse

Immunogen

A synthetic peptide of human RAD50

Host

Rabbit

Applications

WB, IHC, IF

Recommended dilution

WB 1:500 – 1:2000
IHC 1:50 – 1:200
IF 1:50 – 1:200

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

Predicted: 154kDa/Observed: Refer to Figures

Formulation

RAD50 antibody was tube-contained.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Purification

RAD50 antibody was purified using affinity purification.

Storage

Store at -20 Celsius degree. Avoid freeze / thaw cycles.

Alternative antibody names

NBSLD antibody, RAD502 antibody, hRad50 antibody

Database links

Human UniProt/Swiss-Prot:Q92878

Protein names

NBSLD, RAD502, hRad50

Protein function

Component of the MRN complex, which plays a central role in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity and meiosis. The complex possesses single-strand endonuclease activity and double-strand-specific 3′-5′ exonuclease activity, which are provided by MRE11A. RAD50 may be required to bind DNA ends and hold them in close proximity. This could facilitate searches for short or long regions of sequence homology in the recombining DNA templates, and may also stimulate the activity of DNA ligases and/or restrict the nuclease activity of MRE11A to prevent nucleolytic degradation past a given point . The complex may also be required for DNA damage signaling via activation of the ATM kinase . In telomeres the MRN complex may modulate t-loop formation .

Protein tissue specificity

Expressed at very low level in most tissues, except in testis where it is expressed at higher level. Expressed in fibroblasts.

Protein sequence and domain

The zinc-hook, which separates the large intramolecular coiled coil regions, contains 2 Cys residues that coordinate one molecule of zinc with the help of the 2 Cys residues of the zinc-hook of another RAD50 molecule, thereby forming a V-shaped homodimer. The two heads of the homodimer, which constitute the ATP-binding domain, interact with the MRE11A homodimer (By similarity).Belongs to the SMC family. RAD50 subfamily.; Contains 1 zinc-hook domain.

Protein cellular localization

Nucleus . Chromosome, telomere . Note: Localizes to discrete nuclear foci after treatment with genotoxic agents.

Background

The protein encoded by this gene is highly similar to Saccharomyces cerevisiae Rad50, a protein involved in DNA double-strand break repair. This protein forms a complex with MRE11 and NBS1. The protein complex binds to DNA and displays numerous enzymatic activities that are required for nonhomologous joining of DNA ends. This protein, cooperating with its partners, is important for DNA double-strand break repair, cell cycle checkpoint activation, telomere maintenance, and meiotic recombination. Knockout studies of the mouse homolog suggest this gene is essential for cell growth and viability. Mutations in this gene are the cause of Nijmegen breakage syndrome-like disorder.

Research area

All research areas>Tumor Suppressor/Apoptosis>Rad
(View all antibody categories related to Tumor Suppressor/Apoptosis)

Note

AntibodyPlus can customize RAD50 antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provides antibody trial sample for your own antibody validation.

Product type

Primary antibody

Supplier

ABclonal Inc.

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