Rabbit polyclonal RAD9A antibody (A1890)

$69.00$259.00

Reactivity: Human,Mouse,Rat
Applications: WB,IHC,IF
Conjugation: Unconjugated
Supplier: ABclonal Inc.

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Rabbit polyclonal RAD9A antibody (A1890)

Supplier: ABclonal Inc.

Recommended applications: WB,IHC,IF

Recommended dilution:

WB 1:500 – 1:2000 IHC 1:50 – 1:200 IF 1:50 – 1:200

Recommended protocols: check protocols

Image descriptions:

Click or hover above images to see image description for Rabbit polyclonal RAD9A antibody.

Alternative names:

Check alternative names for the antibody

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RAD9 antibody
Cell cycle checkpoint control protein antibody|Cell cycle checkpoint control protein RAD9A antibody|DNA repair exonuclease rad9 homolog A antibody|hRAD 9 antibody|hRAD9 antibody|Rad 9 antibody|RAD 9A antibody|RAD9 (S pombe) homolog antibody|RAD9 homolog A antibody|RAD9 homolog antibody|Rad9a antibody|RAD9A_HUMAN antibody|Anti-Rad9 antibody [93A535] (ab13600)

SCBT cat No: sc-136053|sc-74463|sc-166457|sc-74464|sc-10465|sc-8324|sc-32489|

Name

Rabbit polyclonal RAD9A antibody

Catalogue No.

A1890

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant protein of human RAD9A

Host

Rabbit

Applications

WB, IHC, IF

Recommended dilution

WB 1:500 – 1:2000
IHC 1:50 – 1:200
IF 1:50 – 1:200

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

Predicted: 42kDa/Observed: Refer to Figures

Formulation

RAD9A antibody was tube-contained.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Purification

RAD9A antibody was purified using affinity purification.

Storage

Store at -20 Celsius degree. Avoid freeze / thaw cycles.

Alternative antibody names

RAD9 antibody

Database links

Human UniProt/Swiss-Prot:Q99638

Protein names

RAD9

Protein function

Component of the 9-1-1 cell-cycle checkpoint response complex that plays a major role in DNA repair. The 9-1-1 complex is recruited to DNA lesion upon damage by the RAD17-replication factor C (RFC) clamp loader complex. Acts then as a sliding clamp platform on DNA for several proteins involved in long-patch base excision repair (LP-BER). The 9-1-1 complex stimulates DNA polymerase beta (POLB) activity by increasing its affinity for the 3′-OH end of the primer-template and stabilizes POLB to those sites where LP-BER proceeds; endonuclease FEN1 cleavage activity on substrates with double, nick, or gap flaps of distinct sequences and lengths; and DNA ligase I (LIG1) on long-patch base excision repair substrates. The 9-1-1 complex is necessary for the recruitment of RHNO1 to sites of double-stranded breaks (DSB) occurring during the S phase. RAD9A possesses 3′->5′ double stranded DNA exonuclease activity. Its phosphorylation by PRKCD may be required for the formation of the 9-1-1 complex.

Protein sequence and domain

Belongs to the rad9 family.

Protein post-translational modifications

Constitutively phosphorylated on serine and threonine amino acids in absence of DNA damage. Hyperphosphorylated by PRKCD and ABL1 upon DNA damage. Its phosphorylation by PRKCD may be required for the formation of the 9-1-1 complex.

Protein cellular localization

Nucleus .

Background

This gene product is highly similar to Schizosaccharomyces pombe rad9, a cell cycle checkpoint protein required for cell cycle arrest and DNA damage repair. This protein possesses 3′ to 5′ exonuclease activity, which may contribute to its role in sensing and repairing DNA damage. It forms a checkpoint protein complex with RAD1 and HUS1. This complex is recruited by checkpoint protein RAD17 to the sites of DNA damage, which is thought to be important for triggering the checkpoint-signaling cascade. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2011]

Research area

All research areas>Tumor Suppressor/Apoptosis>Rad
(View all antibody categories related to Tumor Suppressor/Apoptosis)

Note

AntibodyPlus can customize RAD9A antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provides antibody trial sample for your own antibody validation.

Product type

Primary antibody

Supplier

ABclonal Inc.

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