Rabbit Polyclonal RANKL antibody (STJ96769)

$99.00$319.00

Reactivity: Human, Mouse, Rat
Applications: WB, IHC
Conjugation: Unconjugated
Supplier: St John’s Laboratory Ltd.

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Rabbit Polyclonal RANKL antibody (STJ96769)

Supplier: St John’s Laboratory Ltd.

Recommended applications: WB, IHC, ELISA

Recommended dilution: WB 1:500-1:2000; IHC-P 1:100-1:300; ELISA 1:20000;

Recommended protocols: check protocols

Image descriptions:

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Alternative names:

Check alternative names for the antibody

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TNFSF11 antibody, OPGL antibody, RANKL antibody, TRANCE antibody,|CD254 antibody|TNF11_HUMAN antibody|TNFSF 11 antibody|Tnfsf11 antibody|TRANCE antibody|Tumor necrosis factor (ligand) superfamily member 11 antibody|Tumor necrosis factor ligand superfamily member 11 antibody|Tumor necrosis factor ligand superfamily member 11, soluble form antibody|Anti-RANKL antibody [12A668] – BSA and Azide free (ab45039)
SCBT cat No: sc-52950|

Name

RANKL Polyclonal Antibody

Catalogue No.

STJ96769

Reactivity

Human, Mouse, Rat

Specificity

RANKL Polyclonal Antibody detects endogenous levels of RANKL protein.

Immunogen

Synthesized peptide derived from Tumor necrosis factor ligand superfamily member 11 at AA range 261-310

Host

Rabbit

Applications

WB, IHC, ELISA

Recommended dilution

WB 1:500-1:2000; IHC-P 1:100-1:300; ELISA 1:20000;

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

35 kDa

Formulation

RANKL Antibody was tube-contained. Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Concentration

1 mg/ml

Purification

RANKL Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage

-20 Celsius degree. Avoid repeated freeze/thaw cycles.

Alternative antibody names

Tumor necrosis factor ligand superfamily member 11 antibody, Osteoclast differentiation factor antibody, ODF antibody, Osteoprotegerin ligand antibody, OPGL antibody, Receptor activator of nuclear factor kappa-B ligand antibody, RANKL antibody, TNF-related activation-induced cytokine antibody, TRANCE antibody, CD antigen CD254 antibody

Database links

Human UniProt/Swiss-Prot:O14788;Mouse UniPort/Swiss-Prot: O35235;Rat UniProt/Swiss-Port: Q9ESE2;Human Entrez Gene: 8600;Mouse Entrez Gene: 21943;Rat Entrez Gene: Rn.217570

Protein names

Tumor necrosis factor ligand superfamily member 11 , Osteoclast differentiation factor , ODF , Osteoprotegerin ligand , OPGL , Receptor activator of nuclear factor kappa-B ligand , RANKL , TNF-related activation-induced cytokine , TRANCE , CD antigen CD254

Protein function

Cytokine that binds to TNFRSF11B/OPG and to TNFRSF11A/RANK. Osteoclast differentiation and activation factor. Augments the ability of dendritic cells to stimulate naive T-cell proliferation. May be an important regulator of interactions between T-cells and dendritic cells and may play a role in the regulation of the T-cell-dependent immune response. May also play an important role in enhanced bone-resorption in humoral hypercalcemia of malignancy . Induces osteoclastogenesis by activating multiple signaling pathways in osteoclast precursor cells, chief among which is induction of long lasting oscillations in the intracellular concentration of Ca (2+) resulting in the activation of NFATC1, which translocates to the nucleus and induces osteoclast-specific gene transcription to allow differentiation of osteoclasts. During osteoclast differentiation, in a TMEM64 and ATP2A2-dependent manner induces activation of CREB1 and mitochondrial ROS generation necessary for proper osteoclast generation (By similarity).

Protein tissue specificity

Highest in the peripheral lymph nodes, weak in spleen, peripheral blood Leukocytes, bone marrow, heart, placenta, skeletal muscle, stomach and thyroid.

Involvement in disease

Osteopetrosis, autosomal recessive 2 (OPTB2) [MIM:259710]: A rare genetic disease characterized by abnormally dense bone, due to defective resorption of immature bone. Osteopetrosis occurs in two forms: a severe autosomal recessive form occurring in utero, infancy, or childhood, and a benign autosomal dominant form occurring in adolescence or adulthood. Recessive osteopetrosis commonly manifests in early infancy with macrocephaly, feeding difficulties, evolving blindness and deafness, bone marrow failure, severe anemia, and hepatosplenomegaly. Deafness and blindness are generally thought to represent effects of pressure on nerves. OPTB2 is characterized by paucity of osteoclasts, suggesting a molecular defect in osteoclast development. . Note: The disease is caused by mutations affecting the gene represented in this entry.

Protein sequence and domain

Belongs to the tumor necrosis factor family.

Protein post-translational modifications

The soluble form of isoform 1 derives from the membrane form by proteolytic processing (By similarity). The cleavage may be catalyzed by ADAM17.

Protein cellular localization

Cell membrane; Single-pass type II membrane protein / Cell membrane; Single-pass type II membrane protein / Cytoplasm / Secreted

Research area

All research areas>Lymphocyte Signaling>RANK
(View all antibody categories related to Lymphocyte Signaling)

Note

AntibodyPlus can customize RANKL Antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provide antibody trial sample for your own antibody validation and collects antibody reviews.

Supplier

St John’s Laboratory Ltd.

Product type

Primary antibody

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Immunohistochemical analysis of paraffin embedded Human uterus tissue

1: RANKL Polyclonal Antibody was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Rat lung tissue

1: RANKL Polyclonal Antibody was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Mouse kidney tissue

1: RANKL Polyclonal Antibody was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.


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