Rabbit Polyclonal RFWD2 antibody (A10463)

$99.00$259.00

Reactivity: Human,Mouse
Applications: WB
Conjugation: Unconjugated
Supplier: ABclonal Inc.

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Rabbit Polyclonal RFWD2 antibody (A10463)

Supplier: ABclonal Inc.

Recommended applications: WB

Recommended dilution:

WB 1:500 – 1:2000

Recommended protocols: check protocols

Image descriptions:

Click or hover above images to see image description for Rabbit Polyclonal RFWD2 antibody.

Alternative names:

Check alternative names for the antibody

Expand

COP1 antibody; RNF200 antibody;
Constitutive photomorphogenesis protein 1 homolog antibody|COP antibody|E3 ubiquitin-protein ligase RFWD2 antibody|hCOP1 antibody|Rfwd2 antibody|RFWD2_HUMAN antibody|ring finger and WD repeat domain 2 antibody|RING finger and WD repeat domain protein 2 antibody|RING finger protein 200 antibody|Anti-COP1 antibody (ab56400)

SCBT cat No: sc-166799|sc-34592|

Name

Rabbit Polyclonal RFWD2 antibody

Catalogue No.

A10463

Reactivity

Human, Mouse

Immunogen

Recombinant protein of human RFWD2

Host

Rabbit

Applications

WB

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

80kDa

Formulation

RFWD2 antibody was tube-contained.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Purification

Affinity purification

Storage

Store at -20 Celsius degree. Avoid freeze / thaw cycles.

Alternative antibody names

COP1 antibody; RNF200 antibody;

Protein names

COP1; RNF200;

Protein function

E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Involved in JUN ubiquitination and degradation. Directly involved in p53 (TP53) ubiquitination and degradation, thereby abolishing p53-dependent transcription and apoptosis. Ubiquitinates p53 independently of MDM2 or RCHY1. Probably mediates E3 ubiquitin ligase activity by functioning as the essential RING domain subunit of larger E3 complexes. In contrast, it does not constitute the catalytic RING subunit in the DCX DET1-COP1 complex that negatively regulates JUN, the ubiquitin ligase activity being mediated by RBX1. Involved in 14-3-3 protein sigma/SFN ubiquitination and proteasomal degradation, leading to AKT activation and promotion of cell survival. Ubiquitinates MTA1 leading to its proteasomal degradation.

Protein tissue specificity

Ubiquitously expressed at low level. Expressed at higher level in testis, placenta, skeletal muscle and heart.

Protein sequence and domain

Belongs to the COP1 family.; Contains 1 RING-type zinc finger.; Contains 7 WD repeats. The RING finger domain, in addition to its role in ubiquitination, functions as a structural scaffold to bring two clusters of positive-charged residues within spatial proximity to mimic a bipartite nuclear localization signal (NLS).

Protein post-translational modifications

Autoubiquitinated. MTA1 destabilizes it by promoting its autoubiquitination.

Protein cellular localization

Nucleus speckle. Cytoplasm. Note=In the nucleus, it forms nuclear speckles.

Research area

<a href="http://antibodyplus.com/research-areas/">All research areas</a>><a href="http://antibodyplus.com/Tumor-SuppressorApoptosis/">Tumor Suppressor/Apoptosis</a>><a href="http://antibodyplus.com/product-tag/COP1/">COP1</a><br><a href="http://antibodyplus.com/product-tag/Tumor-SuppressorApoptosis/"&gt; (View all antibody categories related to Tumor Suppressor/Apoptosis)</a>

Note

AntibodyPlus can customize RFWD2 antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provides antibody trial sample for your own antibody validation.

Product type

Primary antibody

Supplier

ABclonal Inc.

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