Rabbit polyclonal ROCK1 antibody (A1008)

$69.00$259.00

Reactivity: Human,Mouse,Rat
Applications: WB,IHC
Conjugation: Unconjugated
Supplier: ABclonal Inc.

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Rabbit polyclonal ROCK1 antibody (A1008)

Supplier: ABclonal Inc.

Recommended applications: WB,IHC

Recommended dilution:

WB 1:500 – 1:2000 IHC 1:50 – 1:100

Recommended protocols: check protocols

Image descriptions:

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Alternative names:

Check alternative names for the antibody

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ROCK-I antibody, P160ROCK antibody
coiled-coil-containing protein kinase 1 antibody| coiled-coil-containing protein kinase I antibody|MGC131603 antibody|MGC43611 antibody|p160 Rhoassociated coiled coil-forming protein kinase antibody|p160 ROCK-1 antibody|p160 ROCK1 antibody|p160ROCK antibody|PRO0435 antibody|Renal carcinoma antigen NY REN 35 antibody|Renal carcinoma antigen NY-REN-35 antibody|Rho associated coiled coil containing protein kinase 1 antibody|Rho associated protein kinase 1 antibody|Rho kinase antibody|Rho-alpha kinase antibody|Rho-associated antibody|Rho-associated protein kinase 1 antibody|ROCK I antibody|ROCK-I antibody|ROCK1 antibody|ROCK1_HUMAN antibody|Rok antibody|rokalpha antibody|Anti-ROCK1 antibody [EP786Y] (ab45171)

SCBT cat No: sc-52953|sc-71965|sc-23465|sc-23464|sc-23335|sc-73850|sc-49413|sc-49414|

Name

Rabbit polyclonal ROCK1 antibody

Catalogue No.

A1008

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant protein of human ROCK1

Host

Rabbit

Applications

WB, IHC

Recommended dilution

WB 1:500 – 1:2000
IHC 1:50 – 1:100

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

Predicted: 158kDa/Observed: Refer to Figures

Formulation

ROCK1 antibody was tube-contained.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Purification

ROCK1 antibody was purified using affinity purification.

Storage

Store at -20 Celsius degree. Avoid freeze / thaw cycles.

Alternative antibody names

ROCK-I antibody, P160ROCK antibody

Database links

Human UniProt/Swiss-Prot:Q13464

Protein names

ROCK-I, P160ROCK

Protein function

Protein kinase which is a key regulator of actin cytoskeleton and cell polarity. Involved in regulation of smooth muscle contraction, actin cytoskeleton organization, stress fiber and focal adhesion formation, neurite retraction, cell adhesion and motility via phosphorylation of DAPK3, GFAP, LIMK1, LIMK2, MYL9/MLC2, PFN1 and PPP1R12A. Phosphorylates FHOD1 and acts synergistically with it to promote SRC-dependent non-apoptotic plasma membrane blebbing. Phosphorylates JIP3 and regulates the recruitment of JNK to JIP3 upon UVB-induced stress. Acts as a suppressor of inflammatory cell migration by regulating PTEN phosphorylation and stability. Acts as a negative regulator of VEGF-induced angiogenic endothelial cell activation. Required for centrosome positioning and centrosome-dependent exit from mitosis. Plays a role in terminal erythroid differentiation. May regulate closure of the eyelids and ventral body wall by inducing the assembly of actomyosin bundles. Promotes keratinocyte terminal differentiation. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization.

Protein tissue specificity

Detected in blood platelets.

Protein sequence and domain

The C-terminal auto-inhibitory domain interferes with kinase activity. RHOA binding leads to a conformation change and activation of the kinase. Truncated ROCK1 is constitutively activated.Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.; Contains 1 AGC-kinase C-terminal domain.; Contains 1 PH domain.; Contains 1 phorbol-ester/DAG-type zinc finger.; Contains 1 protein kinase domain.; Contains 1 REM (Hr1) repeat.

Protein post-translational modifications

Autophosphorylated on serine and threonine residues.; Cleaved by caspase-3 during apoptosis. This leads to constitutive activation of the kinase and membrane blebbing.

Protein cellular localization

Cytoplasm. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole . Golgi apparatus membrane; Peripheral membrane protein. Cell projection, bleb. Cytoplasm, cytoskeleton . Cell membrane . Cell projection, lamellipodium . Cell projection, ruffle . Note: Associated with the mother centriole and an intercentriolar linker. Colocalizes with ITGB1BP1 and ITGB1 at the cell membrane predominantly in lamellipodia and membrane ruffles, but also in retraction fibers. Localizes at the cell membrane in an ITGB1BP1-dependent manner (By similarity). A small proportion is associated with Golgi membranes.

Background

Rho-associated kinase I (ROCK1, ROK beta, p160ROCK) is a serine-threonine protein kinase and an effector of the small GTPase Rho. With N-terminus protein kinase domain and C-terminus Rho-binding domain/pleckstrin homology domain, ROCK1 can be activated by either RhoA or RhoB. In Rho specific ROCK1 activation, a Rho protein binds to the Rho-binding domain and induces a conformational change which opens the kinase domain for the phosphorylation of downstream effectors (1). Activated ROCK1 phosphorylates various signaling proteins, such as myosin light chain phosphatase, LIM kinases, and ezrin-radixin-moesin proteins. Caspase-3 also activates ROCK1; Caspase-3 cleaves ROCK1 at DETD1113/G sequence and removes its inhibitory c-terminal domain. This activation, independent of Rho activity, leads to apoptotic membrane blebbing (2). ROCK1 is involved in regulating actin cytoskeleton assembly, cell migration, centromere positing, smooth muscle contraction, and neurite outgrowth. Its involvement in tumor invasion, hypertension, and bronchial asthma makes ROCK1 an ideal target for drug development (3).

Research area

All research areas>Kinases and Phosphatases>Rock
(View all antibody categories related to Kinases and Phosphatases)

Note

AntibodyPlus can customize ROCK1 antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provides antibody trial sample for your own antibody validation.

Product type

Primary antibody

Supplier

ABclonal Inc.

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