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Rabbit polyclonal ROCK1 antibody (A1008)
Supplier: ABclonal Inc.
Recommended applications: WB,IHC
WB 1:500 – 1:2000 IHC 1:50 – 1:100
Recommended protocols: check protocols
Click or hover above images to see image description for Rabbit polyclonal ROCK1 antibody.
Check alternative names for the antibodyExpand
ROCK-I antibody, P160ROCK antibody
coiled-coil-containing protein kinase 1 antibody| coiled-coil-containing protein kinase I antibody|MGC131603 antibody|MGC43611 antibody|p160 Rhoassociated coiled coil-forming protein kinase antibody|p160 ROCK-1 antibody|p160 ROCK1 antibody|p160ROCK antibody|PRO0435 antibody|Renal carcinoma antigen NY REN 35 antibody|Renal carcinoma antigen NY-REN-35 antibody|Rho associated coiled coil containing protein kinase 1 antibody|Rho associated protein kinase 1 antibody|Rho kinase antibody|Rho-alpha kinase antibody|Rho-associated antibody|Rho-associated protein kinase 1 antibody|ROCK I antibody|ROCK-I antibody|ROCK1 antibody|ROCK1_HUMAN antibody|Rok antibody|rokalpha antibody|Anti-ROCK1 antibody [EP786Y] (ab45171)
SCBT cat No: sc-52953|sc-71965|sc-23465|sc-23464|sc-23335|sc-73850|sc-49413|sc-49414|
Rabbit polyclonal ROCK1 antibody
|Catalogue No.|| |
Human, Mouse, Rat
Recombinant protein of human ROCK1
|Recommended dilution|| |
WB 1:500 – 1:2000
|Molecular weight|| |
Predicted: 158kDa/Observed: Refer to Figures
ROCK1 antibody was tube-contained.
ROCK1 antibody was purified using affinity purification.
Store at -20 Celsius degree. Avoid freeze / thaw cycles.
|Alternative antibody names|| |
ROCK-I antibody, P160ROCK antibody
|Database links|| |
|Protein names|| |
|Protein function|| |
Protein kinase which is a key regulator of actin cytoskeleton and cell polarity. Involved in regulation of smooth muscle contraction, actin cytoskeleton organization, stress fiber and focal adhesion formation, neurite retraction, cell adhesion and motility via phosphorylation of DAPK3, GFAP, LIMK1, LIMK2, MYL9/MLC2, PFN1 and PPP1R12A. Phosphorylates FHOD1 and acts synergistically with it to promote SRC-dependent non-apoptotic plasma membrane blebbing. Phosphorylates JIP3 and regulates the recruitment of JNK to JIP3 upon UVB-induced stress. Acts as a suppressor of inflammatory cell migration by regulating PTEN phosphorylation and stability. Acts as a negative regulator of VEGF-induced angiogenic endothelial cell activation. Required for centrosome positioning and centrosome-dependent exit from mitosis. Plays a role in terminal erythroid differentiation. May regulate closure of the eyelids and ventral body wall by inducing the assembly of actomyosin bundles. Promotes keratinocyte terminal differentiation. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization.
|Protein tissue specificity|| |
Detected in blood platelets.
|Protein sequence and domain|| |
The C-terminal auto-inhibitory domain interferes with kinase activity. RHOA binding leads to a conformation change and activation of the kinase. Truncated ROCK1 is constitutively activated.Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.; Contains 1 AGC-kinase C-terminal domain.; Contains 1 PH domain.; Contains 1 phorbol-ester/DAG-type zinc finger.; Contains 1 protein kinase domain.; Contains 1 REM (Hr1) repeat.
|Protein post-translational modifications|| |
Autophosphorylated on serine and threonine residues.; Cleaved by caspase-3 during apoptosis. This leads to constitutive activation of the kinase and membrane blebbing.
|Protein cellular localization|| |
Cytoplasm. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole . Golgi apparatus membrane; Peripheral membrane protein. Cell projection, bleb. Cytoplasm, cytoskeleton . Cell membrane . Cell projection, lamellipodium . Cell projection, ruffle . Note: Associated with the mother centriole and an intercentriolar linker. Colocalizes with ITGB1BP1 and ITGB1 at the cell membrane predominantly in lamellipodia and membrane ruffles, but also in retraction fibers. Localizes at the cell membrane in an ITGB1BP1-dependent manner (By similarity). A small proportion is associated with Golgi membranes.
Rho-associated kinase I (ROCK1, ROK beta, p160ROCK) is a serine-threonine protein kinase and an effector of the small GTPase Rho. With N-terminus protein kinase domain and C-terminus Rho-binding domain/pleckstrin homology domain, ROCK1 can be activated by either RhoA or RhoB. In Rho specific ROCK1 activation, a Rho protein binds to the Rho-binding domain and induces a conformational change which opens the kinase domain for the phosphorylation of downstream effectors (1). Activated ROCK1 phosphorylates various signaling proteins, such as myosin light chain phosphatase, LIM kinases, and ezrin-radixin-moesin proteins. Caspase-3 also activates ROCK1; Caspase-3 cleaves ROCK1 at DETD1113/G sequence and removes its inhibitory c-terminal domain. This activation, independent of Rho activity, leads to apoptotic membrane blebbing (2). ROCK1 is involved in regulating actin cytoskeleton assembly, cell migration, centromere positing, smooth muscle contraction, and neurite outgrowth. Its involvement in tumor invasion, hypertension, and bronchial asthma makes ROCK1 an ideal target for drug development (3).
AntibodyPlus can customize ROCK1 antibody according to your requirement, including bulk product size,etc. Please contact firstname.lastname@example.org. AntibodyPlus provides antibody trial sample for your own antibody validation.
|Product type|| |
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