Rabbit polyclonal ROCK2 antibody (A5698)

$69.00$259.00

Reactivity: Human,Mouse,Rat
Applications: WB,IHC,IF,IP
Conjugation: Unconjugated
Supplier: ABclonal Inc.

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Rabbit polyclonal ROCK2 antibody (A5698)

Supplier: ABclonal Inc.

Recommended applications: WB,IHC,IF,IP

Recommended dilution:

WB 1:500 – 1:2000 IHC 1:50 – 1:200 IF 1:50 – 1:200 IP 1:20 – 1:50

Recommended protocols: check protocols

Image descriptions:

Click or hover above images to see image description for Rabbit polyclonal ROCK2 antibody.

Alternative names:

Check alternative names for the antibody

Expand

ROCK-II antibody
coiled-coil-containing protein kinase 2 antibody|KIAA0619 antibody|p164 ROCK 2 antibody|p164 ROCK-2 antibody|Rho associated coiled coil containing protein kinase 2 antibody|Rho associated protein kinase 2 antibody|Rho associated, coiled coil containing protein kinase II antibody|Rho kinase 2 antibody|Rho-associated antibody|Rho-associated protein kinase 2 antibody|ROCK 2 antibody|Rock II antibody|Rock2 antibody|ROCK2_HUMAN antibody|Rock2m antibody|ROK alpha antibody|ROKalpha antibody|Anti-ROCK2 antibody (ab71598)

SCBT cat No: sc-100425|sc-1851|sc-398519|sc-365275|sc-5561|sc-1852|

Name

Rabbit polyclonal ROCK2 antibody

Catalogue No.

A5698

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant protein of human ROCK2

Host

Rabbit

Applications

WB, IHC, IF, IP

Recommended dilution

WB 1:500 – 1:2000
IHC 1:50 – 1:200
IF 1:50 – 1:200
IP 1:20 – 1:50

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

Predicted: 160kDa/Observed: Refer to Figures

Formulation

ROCK2 antibody was tube-contained.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Purification

ROCK2 antibody was purified using affinity purification.

Storage

Store at -20 Celsius degree. Avoid freeze / thaw cycles.

Alternative antibody names

ROCK-II antibody

Database links

Human UniProt/Swiss-Prot:O75116

Protein names

ROCK-II

Protein function

Protein kinase which is a key regulator of actin cytoskeleton and cell polarity. Involved in regulation of smooth muscle contraction, actin cytoskeleton organization, stress fiber and focal adhesion formation, neurite retraction, cell adhesion and motility via phosphorylation of ADD1, BRCA2, CNN1, EZR, DPYSL2, EP300, MSN, MYL9/MLC2, NPM1, RDX, PPP1R12A and VIM. Phosphorylates SORL1 and IRF4. Acts as a negative regulator of VEGF-induced angiogenic endothelial cell activation. Positively regulates the activation of p42/MAPK1-p44/MAPK3 and of p90RSK/RPS6KA1 during myogenic differentiation. Plays an important role in the timely initiation of centrosome duplication. Inhibits keratinocyte terminal differentiation. May regulate closure of the eyelids and ventral body wall through organization of actomyosin bundles. Plays a critical role in the regulation of spine and synaptic properties in the hippocampus. Plays an important role in generating the circadian rhythm of the aortic myofilament Ca(2+) sensitivity and vascular contractility by modulating the myosin light chain phosphorylation.

Protein sequence and domain

An interaction between Thr-414 and Asp-48 is essential for kinase activity and dimerization.Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.; Contains 1 AGC-kinase C-terminal domain.; Contains 1 PH domain.; Contains 1 phorbol-ester/DAG-type zinc finger.; Contains 1 protein kinase domain.; Contains 1 REM (Hr1) repeat.

Protein post-translational modifications

Phosphorylation at Tyr-722 reduces its binding to RHOA and is crucial for focal adhesion dynamics. Dephosphorylation by PTPN11 stimulates its RHOA binding activity.; Cleaved by granzyme B during apoptosis. This leads to constitutive activation of the kinase and membrane blebbing.

Protein cellular localization

Cytoplasm. Cell membrane ; Peripheral membrane protein . Nucleus. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Note: Cytoplasmic, and associated with actin microfilaments and the plasma membrane.

Background

The protein encoded by this gene is a serine/threonine kinase that regulates cytokinesis, smooth muscle contraction, the formation of actin stress fibers and focal adhesions, and the activation of the c-fos serum response element. This protein, which is an isozyme of ROCK1 is a target for the small GTPase Rho.

Research area

All research areas>Kinases and Phosphatases>Rock
(View all antibody categories related to Kinases and Phosphatases)

Note

AntibodyPlus can customize ROCK2 antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provides antibody trial sample for your own antibody validation.

Product type

Primary antibody

Supplier

ABclonal Inc.

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