Rabbit polyclonal RPS3 antibody (A2533)

$69.00$259.00

Reactivity: Human,Mouse,Rat
Applications: WB,IHC,IF
Conjugation: Unconjugated
Supplier: ABclonal Inc.

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Rabbit polyclonal RPS3 antibody (A2533)

Supplier: ABclonal Inc.

Recommended applications: WB,IHC,IF

Recommended dilution:

WB 1:500 – 1:2000 IHC 1:50 – 1:200 IF 1:50 – 1:200

Recommended protocols: check protocols

Image descriptions:

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Alternative names:

Check alternative names for the antibody

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S3 antibody
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SCBT cat No: sc-376008|sc-376098|sc-135390|sc-169173|

Name

Rabbit polyclonal RPS3 antibody

Catalogue No.

A2533

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant protein of human RPS3

Host

Rabbit

Applications

WB, IHC, IF

Recommended dilution

WB 1:500 – 1:2000
IHC 1:50 – 1:200
IF 1:50 – 1:200

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

Predicted: 26kDa/Observed: Refer to Figures

Formulation

RPS3 antibody was tube-contained.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Purification

RPS3 antibody was purified using affinity purification.

Storage

Store at -20 Celsius degree. Avoid freeze / thaw cycles.

Alternative antibody names

S3 antibody

Database links

Human UniProt/Swiss-Prot:P23396

Protein names

S3

Protein function

Involved in translation as a component of the 40S small ribosomal subunit . Has endonuclease activity and plays a role in repair of damaged DNA . Cleaves phosphodiester bonds of DNAs containing altered bases with broad specificity and cleaves supercoiled DNA more efficiently than relaxed DNA . Displays high binding affinity for 7,8-dihydro-8-oxoguanine (8-oxoG), a common DNA lesion caused by reactive oxygen species (ROS) . Has also been shown to bind with similar affinity to intact and damaged DNA . Stimulates the N-glycosylase activity of the base excision protein OGG1 . Enhances the uracil excision activity of UNG1 . Also stimulates the cleavage of the phosphodiester backbone by APEX1 . When located in the mitochondrion, reduces cellular ROS levels and mitochondrial DNA damage . Has also been shown to negatively regulate DNA repair in cells exposed to hydrogen peroxide . Plays a role in regulating transcription as part of the NF-kappa-B p65-p50 complex where it binds to the RELA/p65 subunit, enhances binding of the complex to DNA and promotes transcription of target genes . Represses its own translation by binding to its cognate mRNA . Binds to and protects TP53/p53 from MDM2-mediated ubiquitination . Involved in spindle formation and chromosome movement during mitosis by regulating microtubule polymerization . Involved in induction of apoptosis through its role in activation of CASP8 . Induces neuronal apoptosis by interacting with the E2F1 transcription factor and acting synergistically with it to up-regulate pro-apoptotic proteins BCL2L11/BIM and HRK/Dp5 . Interacts with TRADD following exposure to UV radiation and induces apoptosis by caspase-dependent JNK activation .

Protein sequence and domain

Belongs to the ribosomal protein S3P family.; Contains 1 KH type-2 domain.

Protein post-translational modifications

Methylation by PRMT1 is required for import into the nucleolus and for ribosome assembly.; Sumoylation by SUMO1 enhances protein stability through increased resistance to proteolysis. Sumoylation occurs at one or more of the three consensus sites, Lys-18, Lys-214 and Lys-230.; Phosphorylation at Thr-221 by CDK1 occurs mainly in G2/M phase . Phosphorylation by PRKCD occurs on a non-ribosomal-associated form which results in translocation of RPS3 to the nucleus and enhances its endonuclease activity . Phosphorylated on Ser-209 by IKKB in response to activation of the NF-kappa-B p65-p50 complex which enhances the association of RPS3 with importin-alpha and mediates the nuclear translocation of RPS3 . Phosphorylation by MAPK is required for translocation to the nucleus following exposure of cells to DNA damaging agents such as hydrogen peroxide . Phosphorylation by PKB/AKT mediates RPS3 nuclear translocation, enhances RPS3 endonuclease activity and suppresses RPS3-induced neuronal apoptosis .; Ubiquitinated. This is prevented by interaction with HSP90 which stabilizes the protein.

Protein cellular localization

Cytoplasm . Nucleus . Nucleus, nucleolus . Mitochondrion inner membrane ; Peripheral membrane protein . Cytoplasm, cytoskeleton, spindle . Note: In normal cells, located mainly in the cytoplasm with small amounts in the nucleus but translocates to the nucleus in cells undergoing apoptosis (By similarity). Nuclear translocation is induced by DNA damaging agents such as hydrogen peroxide . Accumulates in the mitochondrion in response to increased ROS levels . Localizes to the spindle during mitosis . Localized in cytoplasmic mRNP granules containing untranslated mRNAs .

Background

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit, where it forms part of the domain where translation is initiated. The protein belongs to the S3P family of ribosomal proteins. Studies of the mouse and rat proteins have demonstrated that the protein has an extraribosomal role as an endonuclease involved in the repair of UV-induced DNA damage. The protein appears to be located in both the cytoplasm and nucleus but not in the nucleolus. Higher levels of expression of this gene in colon adenocarcinomas and adenomatous polyps compared to adjacent normal colonic mucosa have been observed. This gene is co-transcribed with the small nucleolar RNA genes U15A and U15B, which are located in its first and fifth introns, respectively. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Research area

All research areas>Signaling Intermediates>Ribosomal Protein
(View all antibody categories related to Signaling Intermediates)

Note

AntibodyPlus can customize RPS3 antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provides antibody trial sample for your own antibody validation.

Product type

Primary antibody

Supplier

ABclonal Inc.

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