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Rabbit polyclonal RPS3 antibody (A2533)
Supplier: ABclonal Inc.
Recommended applications: WB,IHC,IF
WB 1:500 – 1:2000 IHC 1:50 – 1:200 IF 1:50 – 1:200
Recommended protocols: check protocols
Click or hover above images to see image description for Rabbit polyclonal RPS3 antibody.
Check alternative names for the antibodyExpand
40S ribosomal protein S3 antibody|fb13d09 antibody|FLJ26283 antibody|FLJ27450 antibody|IMR 90 ribosomal protein S3 antibody|MGC56088 antibody|MGC87870 antibody|OTTHUMP00000229804 antibody|OTTHUMP00000229805 antibody|OTTHUMP00000229874 antibody|OTTHUMP00000229877 antibody|OTTHUMP00000229878 antibody|OTTHUMP00000229879 antibody|OTTHUMP00000229880 antibody|OTTHUMP00000229882 antibody|OTTHUMP00000229883 antibody|OTTHUMP00000229886 antibody|Ribosomal protein S3 antibody|rps3 antibody|RS3_HUMAN antibody|S3 antibody|wu:fb13d09 antibody|zgc:56088 antibody|Anti-RPS3 antibody (ab77772)
SCBT cat No: sc-376008|sc-376098|sc-135390|sc-169173|
Rabbit polyclonal RPS3 antibody
|Catalogue No.|| |
Human, Mouse, Rat
Recombinant protein of human RPS3
WB, IHC, IF
|Recommended dilution|| |
WB 1:500 – 1:2000
|Molecular weight|| |
Predicted: 26kDa/Observed: Refer to Figures
RPS3 antibody was tube-contained.
RPS3 antibody was purified using affinity purification.
Store at -20 Celsius degree. Avoid freeze / thaw cycles.
|Alternative antibody names|| |
|Database links|| |
|Protein names|| |
|Protein function|| |
Involved in translation as a component of the 40S small ribosomal subunit . Has endonuclease activity and plays a role in repair of damaged DNA . Cleaves phosphodiester bonds of DNAs containing altered bases with broad specificity and cleaves supercoiled DNA more efficiently than relaxed DNA . Displays high binding affinity for 7,8-dihydro-8-oxoguanine (8-oxoG), a common DNA lesion caused by reactive oxygen species (ROS) . Has also been shown to bind with similar affinity to intact and damaged DNA . Stimulates the N-glycosylase activity of the base excision protein OGG1 . Enhances the uracil excision activity of UNG1 . Also stimulates the cleavage of the phosphodiester backbone by APEX1 . When located in the mitochondrion, reduces cellular ROS levels and mitochondrial DNA damage . Has also been shown to negatively regulate DNA repair in cells exposed to hydrogen peroxide . Plays a role in regulating transcription as part of the NF-kappa-B p65-p50 complex where it binds to the RELA/p65 subunit, enhances binding of the complex to DNA and promotes transcription of target genes . Represses its own translation by binding to its cognate mRNA . Binds to and protects TP53/p53 from MDM2-mediated ubiquitination . Involved in spindle formation and chromosome movement during mitosis by regulating microtubule polymerization . Involved in induction of apoptosis through its role in activation of CASP8 . Induces neuronal apoptosis by interacting with the E2F1 transcription factor and acting synergistically with it to up-regulate pro-apoptotic proteins BCL2L11/BIM and HRK/Dp5 . Interacts with TRADD following exposure to UV radiation and induces apoptosis by caspase-dependent JNK activation .
|Protein sequence and domain|| |
Belongs to the ribosomal protein S3P family.; Contains 1 KH type-2 domain.
|Protein post-translational modifications|| |
Methylation by PRMT1 is required for import into the nucleolus and for ribosome assembly.; Sumoylation by SUMO1 enhances protein stability through increased resistance to proteolysis. Sumoylation occurs at one or more of the three consensus sites, Lys-18, Lys-214 and Lys-230.; Phosphorylation at Thr-221 by CDK1 occurs mainly in G2/M phase . Phosphorylation by PRKCD occurs on a non-ribosomal-associated form which results in translocation of RPS3 to the nucleus and enhances its endonuclease activity . Phosphorylated on Ser-209 by IKKB in response to activation of the NF-kappa-B p65-p50 complex which enhances the association of RPS3 with importin-alpha and mediates the nuclear translocation of RPS3 . Phosphorylation by MAPK is required for translocation to the nucleus following exposure of cells to DNA damaging agents such as hydrogen peroxide . Phosphorylation by PKB/AKT mediates RPS3 nuclear translocation, enhances RPS3 endonuclease activity and suppresses RPS3-induced neuronal apoptosis .; Ubiquitinated. This is prevented by interaction with HSP90 which stabilizes the protein.
|Protein cellular localization|| |
Cytoplasm . Nucleus . Nucleus, nucleolus . Mitochondrion inner membrane ; Peripheral membrane protein . Cytoplasm, cytoskeleton, spindle . Note: In normal cells, located mainly in the cytoplasm with small amounts in the nucleus but translocates to the nucleus in cells undergoing apoptosis (By similarity). Nuclear translocation is induced by DNA damaging agents such as hydrogen peroxide . Accumulates in the mitochondrion in response to increased ROS levels . Localizes to the spindle during mitosis . Localized in cytoplasmic mRNP granules containing untranslated mRNAs .
Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit, where it forms part of the domain where translation is initiated. The protein belongs to the S3P family of ribosomal proteins. Studies of the mouse and rat proteins have demonstrated that the protein has an extraribosomal role as an endonuclease involved in the repair of UV-induced DNA damage. The protein appears to be located in both the cytoplasm and nucleus but not in the nucleolus. Higher levels of expression of this gene in colon adenocarcinomas and adenomatous polyps compared to adjacent normal colonic mucosa have been observed. This gene is co-transcribed with the small nucleolar RNA genes U15A and U15B, which are located in its first and fifth introns, respectively. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene.
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|Product type|| |
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