Rabbit polyclonal RPS6KB1 antibody (A2190)

$69.00$259.00

Reactivity: Human,Mouse,Rat
Applications: WB,IHC,IP
Conjugation: Unconjugated
Supplier: ABclonal Inc.

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Rabbit polyclonal RPS6KB1 antibody (A2190)

Supplier: ABclonal Inc.

Recommended applications: WB,IHC,IP

Recommended dilution:

WB 1:500 – 1:2000 IHC 1:50 – 1:200 IP 1:20 – 1:100

Recommended protocols: check protocols

Image descriptions:

Click or hover above images to see image description for Rabbit polyclonal RPS6KB1 antibody.

Alternative names:

Check alternative names for the antibody

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PS6K antibody, S6K antibody, S6K1 antibody, STK14A antibody, p70(S6K)-alpha antibody, p70-S6K antibody, p70-alpha antibody, p70 antibody
70 kDa ribosomal protein S6 kinase 1 antibody|KS6B1_HUMAN antibody|p70 alpha antibody|P70 beta 1 antibody|p70 ribosomal S6 kinase alpha antibody|p70 ribosomal S6 kinase beta 1 antibody|p70 S6 kinase alpha antibody|P70 S6 Kinase antibody|p70 S6 kinase, alpha 1 antibody|p70 S6 kinase, alpha 2 antibody|p70 S6K antibody|p70 S6K-alpha antibody|p70 S6KA antibody|p70(S6K) alpha antibody|p70(S6K)-alpha antibody|p70-alpha antibody|p70-S6K 1 antibody|p70-S6K antibody|P70S6K antibody|P70S6K1 antibody|p70S6Kb antibody|PS6K antibody|Ribosomal protein S6 kinase 70kDa polypeptide 1 antibody|Ribosomal protein S6 kinase beta 1 antibody|Ribosomal protein S6 kinase beta-1 antibody|Ribosomal protein S6 kinase I antibody|RPS6KB1 antibody|S6K antibody|S6K-beta-1 antibody|S6K1 antibody|Serine/threonine kinase 14 alpha antibody|Serine/threonine-protein kinase 14A antibody|STK14A antibody|Anti-S6K1 antibody [E343] (ab32529)

SCBT cat No: sc-393967|sc-230|sc-514501|sc-9027|sc-8418|sc-293269|sc-9381|sc-9379|sc-130833|sc-56190|sc-56191|

Name

Rabbit polyclonal RPS6KB1 antibody

Catalogue No.

A2190

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant protein of human RPS6KB1

Host

Rabbit

Applications

WB, IHC, IP

Recommended dilution

WB 1:500 – 1:2000
IHC 1:50 – 1:200
IP 1:20 – 1:100

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

Predicted: 59kDa/Observed: Refer to Figures

Formulation

RPS6KB1 antibody was tube-contained.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Purification

RPS6KB1 antibody was purified using affinity purification.

Storage

Store at -20 Celsius degree. Avoid freeze / thaw cycles.

Alternative antibody names

PS6K antibody, S6K antibody, S6K1 antibody, STK14A antibody, p70(S6K)-alpha antibody, p70-S6K antibody, p70-alpha antibody, p70 antibody

Database links

Human UniProt/Swiss-Prot:P23443

Protein names

PS6K, S6K, S6K1, STK14A, p70(S6K)-alpha, p70-S6K, p70-alpha, p70

Protein function

Serine/threonine-protein kinase that acts downstream of mTOR signaling in response to growth factors and nutrients to promote cell proliferation, cell growth and cell cycle progression. Regulates protein synthesis through phosphorylation of EIF4B, RPS6 and EEF2K, and contributes to cell survival by repressing the pro-apoptotic function of BAD. Under conditions of nutrient depletion, the inactive form associates with the EIF3 translation initiation complex. Upon mitogenic stimulation, phosphorylation by the mammalian target of rapamycin complex 1 (mTORC1) leads to dissociation from the EIF3 complex and activation. The active form then phosphorylates and activates several substrates in the pre-initiation complex, including the EIF2B complex and the cap-binding complex component EIF4B. Also controls translation initiation by phosphorylating a negative regulator of EIF4A, PDCD4, targeting it for ubiquitination and subsequent proteolysis. Promotes initiation of the pioneer round of protein synthesis by phosphorylating POLDIP3/SKAR. In response to IGF1, activates translation elongation by phosphorylating EEF2 kinase (EEF2K), which leads to its inhibition and thus activation of EEF2. Also plays a role in feedback regulation of mTORC2 by mTORC1 by phosphorylating RICTOR, resulting in the inhibition of mTORC2 and AKT1 signaling. Mediates cell survival by phosphorylating the pro-apoptotic protein BAD and suppressing its pro-apoptotic function. Phosphorylates mitochondrial URI1 leading to dissociation of a URI1-PPP1CC complex. The free mitochondrial PPP1CC can then dephosphorylate RPS6KB1 at Thr-412, which is proposed to be a negative feedback mechanism for the RPS6KB1 anti-apoptotic function. Mediates TNF-alpha-induced insulin resistance by phosphorylating IRS1 at multiple serine residues, resulting in accelerated degradation of IRS1. In cells lacking functional TSC1-2 complex, constitutively phosphorylates and inhibits GSK3B. May be involved in cytoskeletal rearrangement through binding to neurabin. Phosphorylates and activates the pyrimidine biosynthesis enzyme CAD, downstream of MTOR.

Protein tissue specificity

Widely expressed.

Protein sequence and domain

The autoinhibitory domain is believed to block phosphorylation within the AGC-kinase C-terminal domain and the activation loop.; The TOS (TOR signaling) motif is essential for activation by mTORC1.Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.; Contains 1 AGC-kinase C-terminal domain.; Contains 1 protein kinase domain.

Protein post-translational modifications

Phosphorylation at Thr-412 is regulated by mTORC1. The phosphorylation at this site is maintained by an agonist-dependent autophosphorylation mechanism (By similarity). Activated by phosphorylation at Thr-252 by PDPK1. Dephosphorylation by PPP1CC at Thr-412 in mitochondrion.

Protein cellular localization

Cell junction, synapse, synaptosome . Mitochondrion outer membrane. Mitochondrion. Note: Colocalizes with URI1 at mitochondrion.; Isoform Alpha I: Nucleus. Cytoplasm.; Isoform Alpha II: Cytoplasm.

Background

p70 S6 kinase is a mitogen activated Ser/Thr protein kinase that is required for cell growth and G1 cell cycle progression (1,2). p70 S6 kinase phosphorylates the S6 protein of the 40S ribosomal subunit and is involved in translational control of 5′ oligopyrimidine tract mRNAs (1). A second isoform, p85 S6 kinase, is derived from the same gene and is identical to p70 S6 kinase except for 23 extra residues at the amino terminus, which encode a nuclear localizing signal (1). Both isoforms lie on a mitogen activated signaling pathway downstream of phosphoinositide-3 kinase (PI-3K) and the target of rapamycin, FRAP/mTOR, a pathway distinct from the Ras/MAP kinase cascade (1). The activity of p70 S6 kinase is controlled by multiple phosphorylation events located within the catalytic, linker and pseudosubstrate domains (1). Phosphorylation of Thr229 in the catalytic domain and Thr389 in the linker domain are most critical for kinase function (1). Phosphorylation of Thr389, however, most closely correlates with p70 kinase activity in vivo (3). Prior phosphorylation of Thr389 is required for the action of phosphoinositide 3-dependent protein kinase 1 (PDK1) on Thr229 (4,5). Phosphorylation of this site is stimulated by growth factors such as insulin, EGF and FGF, as well as by serum and some G-protein-coupled receptor ligands, and is blocked by wortmannin, LY294002 (PI-3K inhibitor) and rapamycin (FRAP/mTOR inhibitor) (1,6,7). Ser411, Thr421 and Ser424 lie within a Ser-Pro-rich region located in the pseudosubstrate region (1). Phosphorylation at these sites is thought to activate p70 S6 kinase via relief of pseudosubstrate suppression (1,2). Another LY294002 and rapamycin sensitive phosphorylation site, Ser371, is an in vitro substrate for mTOR and correlates well with the activity of a partially rapamycin resistant mutant p70 S6 kinase (8).

Research area

All research areas>Kinases and Phosphatases>p70
(View all antibody categories related to Kinases and Phosphatases)

Note

AntibodyPlus can customize RPS6KB1 antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provides antibody trial sample for your own antibody validation.

Product type

Primary antibody

Supplier

ABclonal Inc.

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