Rabbit polyclonal RUNX1 antibody (A2055)

$69.00$259.00

Reactivity: Human,Mouse,Rat
Applications: WB,IHC,ICC,IF,IP
Conjugation: Unconjugated
Supplier: ABclonal Inc.

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Rabbit polyclonal RUNX1 antibody (A2055)

Supplier: ABclonal Inc.

Recommended applications: WB,IHC,ICC,IF,IP

Recommended dilution:

WB 1:500 – 1:2000 IHC 1:50 – 1:100 ICC 1:50 – 1:200 IF 1:50 – 1:200 IP 1:20 – 1:100

Recommended protocols: check protocols

Image descriptions:

Click or hover above images to see image description for Rabbit polyclonal RUNX1 antibody.

Alternative names:

Check alternative names for the antibody

Expand

AML1 antibody, AML1-EVI-1 antibody, AMLCR1 antibody, CBFA2 antibody, EVI-1 antibody, PEBP2Ab antibody
Acute myeloid leukemia 1 antibody|RUNX 1 antibody|Runx1 antibody|RUNX1_HUMAN antibody|SL3 3 enhancer factor 1 alpha B subunit antibody|SL3-3 enhancer factor 1 alpha B subunit antibody|SL3/AKV core binding factor alpha B subunit antibody|SL3/AKV core-binding factor alpha B subunit antibody|Anti-RUNX1 / AML1 antibody – ChIP Grade (ab23980)

SCBT cat No: sc-293146|sc-137150|sc-137222|sc-271047|sc-271020|sc-137212|

Name

Rabbit polyclonal RUNX1 antibody

Catalogue No.

A2055

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide of human RUNX1

Host

Rabbit

Applications

WB, IHC, ICC, IF, IP

Recommended dilution

WB 1:500 – 1:2000
IHC 1:50 – 1:100
ICC 1:50 – 1:200
IF 1:50 – 1:200
IP 1:20 – 1:100

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

Predicted: 51kDa/Observed: Refer to Figures

Formulation

RUNX1 antibody was tube-contained.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Purification

RUNX1 antibody was purified using affinity purification.

Storage

Store at -20 Celsius degree. Avoid freeze / thaw cycles.

Alternative antibody names

AML1 antibody, AML1-EVI-1 antibody, AMLCR1 antibody, CBFA2 antibody, EVI-1 antibody, PEBP2Ab antibody

Database links

Human UniProt/Swiss-Prot:Q01196

Protein names

AML1, AML1-EVI-1, AMLCR1, CBFA2, EVI-1, PEBP2Ab

Protein function

CBF binds to the core site, 5′-PYGPYGGT-3′, of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, LCK, IL-3 and GM-CSF promoters. The alpha subunit binds DNA and appears to have a role in the development of normal hematopoiesis. Isoform AML-1L interferes with the transactivation activity of RUNX1. Acts synergistically with ELF4 to transactivate the IL-3 promoter and with ELF2 to transactivate the mouse BLK promoter. Inhibits KAT6B-dependent transcriptional activation. Controls the anergy and suppressive function of regulatory T-cells (Treg) by associating with FOXP3. Activates the expression of IL2 and IFNG and down-regulates the expression of TNFRSF18, IL2RA and CTLA4, in conventional T-cells .

Protein tissue specificity

Expressed in all tissues examined except brain and heart. Highest levels in thymus, bone marrow and peripheral blood.

Protein sequence and domain

A proline/serine/threonine rich region at the C-terminus is necessary for transcriptional activation of target genes.Contains 1 Runt domain.

Protein post-translational modifications

Phosphorylated in its C-terminus upon IL-6 treatment. Phosphorylation enhances interaction with KAT6A.; Methylated.; Phosphorylated in Ser-249 Thr-273 and Ser-276 by HIPK2 when associated with CBFB and DNA. This phosphorylation promotes subsequent EP300 phosphorylation.

Protein cellular localization

Nucleus.

Background

AML1 (also known as Runx1, CBFA2, and PEBP2alphaB) is a member of the core binding factor (CBF) family of transcription factors (1,2). It is required for normal development of all hematopoietic lineages (3-5). AML1 forms a heterodimeric DNA binding complex with its partner protein CBFbeta and regulates the expression of cellular genes by binding to promoter and enhancer elements. AML1 is commonly translocated in hematopoietic cancers: chromosomal translocations include t(8;21) AML1-ETO, t(12;21) TEL-AML, and t(8;21) AML-M2 (6). Phosphorylation of AML1 on several potential serine and threonine sites, including Ser249, is thought to occur in an Erk-dependent manner (7,8).

Research area

All research areas>Transcription Regulators>RUNX
(View all antibody categories related to Transcription Regulators)

Note

AntibodyPlus can customize RUNX1 antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provides antibody trial sample for your own antibody validation.

Product type

Primary antibody

Supplier

ABclonal Inc.

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