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Rabbit Polyclonal RUNX2 antibody (STJ96721)
Supplier: St John’s Laboratory Ltd.
Recommended applications: WB, ELISA
Recommended dilution: WB 1:500-1:2000; ELISA 1:20000;
Recommended protocols: check protocols
Click or hover above images to see image description for RUNX2 Polyclonal Antibody.
Check alternative names for the antibodyExpand
RUNX2 antibody, AML3 antibody, CBFA1 antibody, OSF2 antibody, PEBP2A antibody,|Acute myeloid leukemia 3 protein antibody|RUNX2 antibody|RUNX2_HUMAN antibody|SL3 3 enhancer factor 1 alpha A subunit antibody|SL3-3 enhancer factor 1 alpha A subunit antibody|SL3/AKV core binding factor alpha A subunit antibody|SL3/AKV core-binding factor alpha A subunit antibody|Anti-RUNX2 antibody (ab76956)
SCBT cat No: sc-101145|sc-390715|sc-8566|sc-390351|sc-10758|sc-12488|
RUNX2 Polyclonal Antibody
|Catalogue No.|| |
Human, Mouse, Rat
RUNX2 Polyclonal Antibody detects endogenous levels of RUNX2 protein.
Synthesized peptide derived from RUNX2 at AA range 191-240
|Recommended dilution|| |
WB 1:500-1:2000; ELISA 1:20000;
|Molecular weight|| |
RUNX2 Antibody was tube-contained. Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
RUNX2 Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
-20 Celsius degree. Avoid repeated freeze/thaw cycles.
|Alternative antibody names|| |
Runt-related transcription factor 2 antibody, Acute myeloid leukemia 3 protein antibody, Core-binding factor subunit alpha-1 antibody, CBF-alpha-1 antibody, Oncogene AML-3 antibody, Osteoblast-specific transcription factor 2 antibody, OSF-2 antibody, Polyomavirus enhancer-binding protein 2 alpha A subunit antibody, PEA2-alpha A antibody, PEBP2-alpha A antibody, SL3-3 enhancer factor 1 alpha A subunit antibody, SL3/AKV core-binding factor alpha A subunit antibody
|Database links|| |
Human UniProt/Swiss-Prot:<a href="http://www.uniprot.org/uniprot/Q13950">Q13950</a>;Mouse UniPort/Swiss-Prot: <a href="http://www.uniprot.org/uniprot/Q08775">Q08775</a>;Rat UniProt/Swiss-Port: <a href="http://www.uniprot.org/uniprot/F1M9C5">F1M9C5</a>;Human Entrez Gene: <a href="http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=860">860</a>;Mouse Entrez Gene: <a href="http://www.ncbi.nlm.nih.gov/gene/12393">12393</a>;Rat Entrez Gene: <a href="http://www.ncbi.nlm.nih.gov/UniGene/clust.cgi?ORG=Rn&CID=83672">Rn.83672</a>
|Protein names|| |
Runt-related transcription factor 2 , Acute myeloid leukemia 3 protein , Core-binding factor subunit alpha-1 , CBF-alpha-1 , Oncogene AML-3 , Osteoblast-specific transcription factor 2 , OSF-2 , Polyomavirus enhancer-binding protein 2 alpha A subunit , PEA2-alpha A , PEBP2-alpha A , SL3-3 enhancer factor 1 alpha A subunit , SL3/AKV core-binding factor alpha A subunit
|Protein function|| |
Transcription factor involved in osteoblastic differentiation and skeletal morphogenesis. Essential for the maturation of osteoblasts and both intramembranous and endochondral ossification. CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, osteocalcin, osteopontin, bone sialoprotein, alpha 1(I) collagen, LCK, IL-3 and GM-CSF promoters. In osteoblasts, supports transcription activation: synergizes with SPEN/MINT to enhance FGFR2-mediated activation of the osteocalcin FGF-responsive element (OCFRE) (By similarity). Inhibits KAT6B-dependent transcriptional activation.
|Protein tissue specificity|| |
Specifically expressed in osteoblasts.
|Involvement in disease|| |
Cleidocranial dysplasia (CLCD) [MIM:119600]: Autosomal dominant skeletal disorder with high penetrance and variable expressivity. It is due to defective endochondral and intramembranous bone formation. Typical features include hypoplasia/aplasia of clavicles, patent fontanelles, wormian bones (additional cranial plates caused by abnormal ossification of the calvaria), supernumerary teeth, short stature, and other skeletal changes. In some cases defects in RUNX2 are exclusively associated with dental anomalies. . Note: The disease is caused by mutations affecting the gene represented in this entry.; Metaphyseal dysplasia with maxillary hypoplasia with or without brachydactyly (MDMHB) [MIM:156510]: An autosomal dominant bone dysplasia characterized by metaphyseal flaring of long bones, enlargement of the medial halves of the clavicles, maxillary hypoplasia, variable brachydactyly, and dystrophic teeth. . Note: The disease is caused by mutations affecting the gene represented in this entry. Analysis for copy-number variations revealed that a 105 kb duplication within RUNX2 segregated with the MDMHB phenotype in a region with maximum linkage. Real-time PCR for copy-number variation in genomic DNA in eight samples, as well as sequence analysis of fibroblast cDNA from one subject with MDMHB confirmed that affected family members were heterozygous for the presence of an intragenic duplication encompassing exons 3 to 5 of RUNX2. These three exons code for the Q/A domain and the functionally essential DNA-binding Runt domain of RUNX2. The RUNX2 duplication found in individuals with MDMHB leads to a gain of function (PubMed:23290074). .
|Protein sequence and domain|| |
A proline/serine/threonine rich region at the C-terminus is necessary for transcriptional activation of target genes and contains the phosphorylation sites. / Contains 1 Runt domain.
|Protein post-translational modifications|| |
Phosphorylated; probably by MAP kinases (MAPK). Phosphorylation by HIPK3 is required for the SPEN/MINT and FGF2 transactivation during osteoblastic differentiation (By similarity). Phosphorylation at Ser-451 by CDK1 promotes endothelial cell proliferation required for tumor angiogenesis probably by facilitating cell cycle progression. Isoform 3 is phosphorylated on Ser-340.
|Protein cellular localization|| |
|Research area|| |
<a href="http://antibodyplus.com/research-areas/">All research areas</a>><a href="http://antibodyplus.com/Transcription-Regulators/">Transcription Regulators</a>><a href="http://antibodyplus.com/product-tag/RUNX/">RUNX</a><br><a href="http://antibodyplus.com/product-tag/Transcription-Regulators/"> (View all antibody categories related to Transcription Regulators)</a>
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St John’s Laboratory Ltd.
|Product type|| |
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Immunofluorescence analysis of Human stomach tissue1: RUNX2 Polyclonal Antibody(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.