Rabbit polyclonal SEPT2 antibody (A5801)


Reactivity: Human,Mouse,Rat
Applications: WB,IHC
Conjugation: Unconjugated
Supplier: ABclonal Inc.

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Rabbit polyclonal SEPT2 antibody (A5801)

Supplier: ABclonal Inc.

Recommended applications: WB,IHC

Recommended dilution:

WB 1:500 – 1:2000 IHC 1:50 – 1:200

Recommended protocols: check protocols

Image descriptions:

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Alternative names:

Check alternative names for the antibody


DIFF6 antibody, NEDD5 antibody, NEDD-5 antibody, Pnutl3 antibody, hNedd5 antibody
DIFF6 antibody|Differentiation 6 (deoxyguanosine triphosphate triphosphohydrolase) antibody|LARP antibody|MGC20394 antibody|Peanut like protein 3 antibody|Peanut-like protein 3 antibody|PNUTL3 antibody|SEP1 antibody|SEPT1 antibody|SEPT1_HUMAN antibody|Septin 1 antibody|Septin-1 antibody|Serologically defined breast cancer antigen NY BR 24 antibody|Serologically defined breast cancer antigen NY-BR-24 antibody|Anti-Septin 2 antibody [EPR12123] (ab179436)

SCBT cat No:


Rabbit polyclonal SEPT2 antibody

Catalogue No.



Human, Mouse, Rat


Recombinant protein of human SEPT2





Recommended dilution

WB 1:500 – 1:2000
IHC 1:50 – 1:200







Molecular weight

Predicted: 41kDa/Observed: Refer to Figures


SEPT2 antibody was tube-contained.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.


SEPT2 antibody was purified using affinity purification.


Store at -20 Celsius degree. Avoid freeze / thaw cycles.

Alternative antibody names

DIFF6 antibody, NEDD5 antibody, NEDD-5 antibody, Pnutl3 antibody, hNedd5 antibody

Database links

Human UniProt/Swiss-Prot:Q15019

Protein names

DIFF6, NEDD5, NEDD-5, Pnutl3, hNedd5

Protein function

Filament-forming cytoskeletal GTPase. Required for normal organization of the actin cytoskeleton. Plays a role in the biogenesis of polarized columnar-shaped epithelium by maintaining polyglutamylated microtubules, thus facilitating efficient vesicle transport, and by impeding MAP4 binding to tubulin. Required for the progression through mitosis. Forms a scaffold at the midplane of the mitotic splindle required to maintain CENPE localization at kinetochores and consequently chromosome congression. During anaphase, may be required for chromosome segregation and spindle elongation. Plays a role in ciliogenesis and collective cell movements. In cilia, required for the integrity of the diffusion barrier at the base of the primary cilium that prevents diffusion of transmembrane proteins between the cilia and plasma membranes: probably acts by regulating the assembly of the tectonic-like complex (also named B9 complex) by localizing TMEM231 protein. May play a role in the internalization of 2 intracellular microbial pathogens, Listeria monocytogenes and Shigella flexneri.

Protein tissue specificity

Widely expressed. Up-regulated in liver cancer.

Protein sequence and domain

Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. Septin GTPase family.; Contains 1 septin-type G (guanine nucleotide-binding) domain.

Protein cellular localization

Cytoplasm . Cytoplasm, cytoskeleton . Cytoplasm, cytoskeleton, spindle . Chromosome, centromere, kinetochore . Cleavage furrow . Midbody . Cytoplasm, cell cortex . Cell projection, cilium membrane . Note: In metaphase cells, localized within the microtubule spindle. At the metaphase plate, in close apposition to the kinetochores of the congressed chromosomes. In cells undergoing cytokinesis, localized to the midbody, the ingressing cleavage furrow, and the central spindle. During bacterial infection, displays a collar shape structure next to actin at the pole of invading bacteria. In epithelial cells, colocalizes with polyglutamylated tubulin around the trans-Golgi network, as well as juxatnuclear and proximal Golgi apparatus. Localizes at the base of the cilia near the morphological distinction between the cilia and plasma membranes.

Research area

All research areas>Signaling Intermediates>Septin
(View all antibody categories related to Signaling Intermediates)


AntibodyPlus can customize SEPT2 antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provides antibody trial sample for your own antibody validation.

Product type

Primary antibody


ABclonal Inc.


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Antibody customer reviews & validation data - 112

Validation data ID: 112

Submission date: 2017-02-10

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Result figure:
Result figure

Figure description:

Immunoblotting detection of septin (SEPT) in total lysates of hTERT-RPE1 cells

Product name

Rabbit polyclonal SEPT2 antibody (A5801)

Product catalog No.


Product type

Primary antibody



Application type

Western Blot

Antibody specificity rating


Antibody overall rating


Your comments

We tested the septin (SEPT) antibody on western blots with total lysates of human retinal epithelial cells (hTert-RPE1, ATCC). The antibody showed strong clean band at the expected molecular weight (41kDa), indicating that it is likely specific. We did not test the specificity of the antibody by CRISPR, RNAi or otherwise, which is the only reason for only the "good" rating of specificity.

Detailed protocol

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