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Rabbit Polyclonal Smad2/3 antibody (STJ95697)
Supplier: St John’s Laboratory Ltd.
Recommended applications: WB, IHC, ELISA
Recommended dilution: WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:10000;
Recommended protocols: check protocols
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Check alternative names for the antibodyExpand
SMAD2 antibody, MADH2 antibody, MADR2 antibody,|Drosophila, homolog of, MADR2 antibody|SMAD 2 antibody|SMAD family member 2 antibody|SMAD, mothers against DPP homolog 2 antibody|SMAD2 antibody|SMAD2_HUMAN antibody|Anti-Smad2 antibody [EP784Y] (ab40855)
SCBT cat No: sc-101801|sc-11769|sc-130218|sc-514317|
Smad2/3 Polyclonal Antibody
|Catalogue No.|| |
Human, Mouse, Rat, Monkey
Smad2/3 Polyclonal Antibody detects endogenous levels of Smad2/3 protein.
Synthesized peptide derived from Smad2/3 at AA range 1-80
WB, IHC, ELISA
|Recommended dilution|| |
WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:10000;
|Molecular weight|| |
Smad2/3 Antibody was tube-contained. Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Smad2/3 Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
-20 Celsius degree. Avoid repeated freeze/thaw cycles.
|Alternative antibody names|| |
Mothers against decapentaplegic homolog 2 antibody, MAD homolog 2 antibody, Mothers against DPP homolog 2 antibody, JV18-1 antibody, Mad-related protein 2 antibody, hMAD-2 antibody, SMAD family member 2 antibody, SMAD 2 antibody, Smad2 antibody, hSMAD2 antibody
|Database links|| |
Human UniProt/Swiss-Prot:<a href="http://www.uniprot.org/uniprot/Q15796">Q15796 </a>;Human Entrez Gene: <a href="http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=4087">4087</a>;
|Protein names|| |
Mothers against decapentaplegic homolog 2 , MAD homolog 2 , Mothers against DPP homolog 2 , JV18-1 , Mad-related protein 2 , hMAD-2 , SMAD family member 2 , SMAD 2 , Smad2 , hSMAD2
|Protein function|| |
Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD2/SMAD4 complex, activates transcription. May act as a tumor suppressor in colorectal carcinoma. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator.
|Protein tissue specificity|| |
Expressed at high levels in skeletal muscle, endothelial cells, heart and placenta.
|Protein sequence and domain|| |
Belongs to the dwarfin/SMAD family. / Contains 1 MH1 (MAD homology 1) domain. / Contains 1 MH2 (MAD homology 2) domain.
|Protein post-translational modifications|| |
Phosphorylated on one or several of Thr-220, Ser-245, Ser-250, and Ser-255. In response to TGF-beta, phosphorylated on Ser-465/467 by TGF-beta and activin type 1 receptor kinases. TGF-beta-induced Ser-465/467 phosphorylation declines progressively in a KMT5A-dependent manner. Able to interact with SMURF2 when phosphorylated on Ser-465/467, recruiting other proteins, such as SNON, for degradation. In response to decorin, the naturally occurring inhibitor of TGF-beta signaling, phosphorylated on Ser-240 by CaMK2. Phosphorylated by MAPK3 upon EGF stimulation; which increases transcriptional activity and stability, and is blocked by calmodulin. Phosphorylated by PDPK1. / In response to TGF-beta, ubiquitinated by NEDD4L; which promotes its degradation (By similarity). Monoubiquitinated, leading to prevent DNA-binding. Deubiquitination by USP15 alleviates inhibition and promotes activation of TGF-beta target genes. / Acetylated on Lys-19 by coactivators in response to TGF-beta signaling, which increases transcriptional activity. Isoform short: Acetylation increases DNA binding activity in vitro and enhances its association with target promoters in vivo. Acetylation in the nucleus by EP300 is enhanced by TGF-beta.
|Protein cellular localization|| |
Cytoplasm / Nucleus
|Research area|| |
<a href="http://antibodyplus.com/research-areas/">All research areas</a>><a href="http://antibodyplus.com/Transcription-Regulators/">Transcription Regulators</a>><a href="http://antibodyplus.com/product-tag/Smad/">Smad</a><br><a href="http://antibodyplus.com/product-tag/Transcription-Regulators/"> (View all antibody categories related to Transcription Regulators)</a>
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St John’s Laboratory Ltd.
|Product type|| |
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Immunohistochemical analysis of paraffin embedded Human stomach tissue1: Smad2/3 Polyclonal Antibody was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.