Rabbit Polyclonal Talin-1 antibody (STJ97271)

$99.00$319.00

Reactivity: Human, Mouse, Rat
Applications: WB, IHC, ELISA
Conjugation: Unconjugated
Supplier: St John’s Laboratory Ltd.

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Rabbit Polyclonal Talin-1 antibody (STJ97271)

Supplier: St John’s Laboratory Ltd.

Recommended applications: WB, IHC, ELISA

Recommended dilution: WB 1:500-1:2000; IHC-P 1:100-1:300; ELISA 1:10000;

Recommended protocols: check protocols

Image descriptions:

Click or hover above images to see image description for Talin-1 Polyclonal Antibody.

Alternative names:

Check alternative names for the antibody

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TLN1 antibody, KIAA1027 antibody, TLN antibody,|ILWEQ antibody|Talin 1 antibody|Talin antibody|Talin-1 antibody|TLN 1 antibody|TLN antibody|Tln1 antibody|TLN1_HUMAN antibody|Anti-Talin 1 antibody (ab71333)
SCBT cat No: To be updated

 

Name

Talin-1 Polyclonal Antibody

Catalogue No.

STJ97271

Reactivity

Human, Mouse, Rat

Specificity

Talin-1 Polyclonal Antibody detects endogenous levels of Talin-1 protein.

Immunogen

Synthesized peptide derived from Talin-1 at AA range c-terminal

Host

Rabbit

Applications

WB, IHC, ELISA

Recommended dilution

WB 1:500-1:2000; IHC-P 1:100-1:300; ELISA 1:10000;

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Formulation

Talin-1 Antibody was tube-contained. Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Concentration

1 mg/ml

Purification

Talin-1 Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage

-20 Celsius degree. Avoid repeated freeze/thaw cycles.

Alternative antibody names

Talin-1 antibody

Database links

Human UniProt/Swiss-Prot:Q9Y490;Mouse UniPort/Swiss-Prot: P26039;Rat UniProt/Swiss-Port: G3V852;Human Entrez Gene: 7094;Mouse Entrez Gene: 21894;Rat Entrez Gene: Rn.7715

Protein names

Talin-1

Protein function

Probably involved in connections of major cytoskeletal structures to the plasma membrane. High molecular weight cytoskeletal protein concentrated at regions of cell-substratum contact and, in lymphocytes, at cell-cell contacts (By similarity).

Protein sequence and domain

Contains 1 FERM domain. / Contains 1 I/LWEQ domain.

Protein cellular localization

Cell projection > ruffle membrane / Cytoplasm > cytoskeleton / Cell surface / Cell junction > focal adhesion

Research area

All research areas>Cell Adhesion Proteins>Talin
(View all antibody categories related to Cell Adhesion Proteins)

Note

AntibodyPlus can customize Talin-1 Antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provide antibody trial sample for your own antibody validation and collects antibody reviews.

Supplier

St John’s Laboratory Ltd.

Product type

Primary antibody

Reviews


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Antibody customer reviews & validation data - 113

Validation data ID: 113

Submission date: 2017-02-10

View Details

Result figure:
Result figure

Figure description:

Immunoblotting detection of talin-1 in total lysates of hTERT-RPE1 cells

Product name

Rabbit Polyclonal Talin-1 antibody

Product catalog No.

STJ97271

Product type

Primary antibody

Supplier

St John's Laboratory Ltd

Application type

Western Blot

Antibody specificity rating

Not work in this case

Antibody overall rating

Not work in this case

Your comments

The antibody showed no specific signal on Western blots with total lysates of human retinal epithelial cells. At long exposure (300s), it showed a weak band at about 30kDa and no signal at the expected size of 269kDa. The same samples developed in parallel with anti-filamin (FLNA) antibody showed strong band at the expected size of about 281 kDa. The test was repeated two times, with essentially the same negative result.

Detailed protocol

View Details

Antibody customer reviews & validation data - 77

Validation data ID: 77

Submission date: 2017-01-20

View Details

Result figure:
Talin1.pdf

Figure description:

Talin1 is present ubiquitously throughout the zebrafish embryonic hearts, and is localized within the cytoplasm of the cells. In the migrating endocardial cell, Talin1 is more enriched in the submembrane of the protrusion, and less dispersed throughout the cytoplasm.
GFP marks membrane of migrating cells, DAPI marks nuclei.
There is strong non-specific background of the antibody signal in the blood cells.

Product name

Rabbit Polyclonal Talin-1 antibody

Product catalog No.

STJ97271

Product type

Primary antibody

Supplier

St John's Laboratory Ltd

Application type

Immunofluorescence (IF)

Antibody specificity rating

Good

Antibody overall rating

Good

Your comments

Talin1 is present ubiquitously throughout the zebrafish embryonic hearts, and is localized within the cytoplasm of the cells. In the migrating endocardial cell, Talin1 is more enriched in the submembrane of the protrusion, and less dispersed throughout the cytoplasm.
There is strong non-specific background of the antibody signal in the blood cells.

Detailed protocol

View Details

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