Rabbit Polyclonal TNF-alpha antibody (STJ96054)

$99.00$319.00

Reactivity: Human, Mouse, Rat
Applications: WB, IF
Conjugation: Unconjugated
Supplier: St John’s Laboratory Ltd.

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Rabbit Polyclonal TNF-alpha antibody (STJ96054)

Supplier: St John’s Laboratory Ltd.

Recommended applications: IHC, IF, ELISA

Recommended dilution: IHC 1:100-1:300; IF 1:200-1:1000; ELISA 1:10000;

Recommended protocols: check protocols

Image descriptions:

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Alternative names:

Check alternative names for the antibody

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TNF antibody, TNFA antibody, TNFSF2 antibody,|APC1 antibody|APC1 protein antibody|Cachectin antibody|DIF antibody|Differentiation inducing factor antibody|Macrophage cytotoxic factor antibody|Tnf antibody|TNF superfamily, member 2 antibody|TNF, macrophage derived antibody|TNF, monocyte derived antibody|TNF-a antibody|TNF-alpha antibody|TNFA antibody|TNFA_HUMAN antibody|TNFSF2 antibody|Tumor necrosis factor alpha antibody|Tumor necrosis factor antibody|Tumor necrosis factor ligand superfamily member 2 antibody|Tumor Necrosis Factor, Membrane Form antibody|Tumor necrosis factor, soluble form antibody|Anti-TNF alpha antibody (ab6671)
SCBT cat No: To be updated

Name

TNF-alpha Polyclonal Antibody

Catalogue No.

STJ96054

Reactivity

Human, Mouse, Rat

Specificity

TNF-alpha Polyclonal Antibody detects endogenous levels of TNF-alpha protein.

Immunogen

Synthesized peptide derived from TNF-alpha at AA range 110-190

Host

Rabbit

Applications

IHC, IF, ELISA

Recommended dilution

IHC 1:100-1:300; IF 1:200-1:1000; ELISA 1:10000;

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

16 kDa

Formulation

TNF-alpha Antibody was tube-contained. Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Concentration

1 mg/ml

Purification

TNF-alpha Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage

-20 Celsius degree. Avoid repeated freeze/thaw cycles.

Alternative antibody names

Tumor necrosis factor antibody, Cachectin antibody, TNF-alpha antibody, Tumor necrosis factor ligand superfamily member 2 antibody, TNF-a antibody

Database links

Human UniProt/Swiss-Prot:P01375;Mouse UniPort/Swiss-Prot: P06804;Rat UniProt/Swiss-Port: P16599;Human Entrez Gene: 7124;Mouse Entrez Gene: 21926;Rat Entrez Gene: Rn.2275

Protein names

Tumor necrosis factor , Cachectin , TNF-alpha , Tumor necrosis factor ligand superfamily member 2 , TNF-a

Protein function

Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. Impairs regulatory T-cells (Treg) function in individuals with rheumatoid arthritis via FOXP3 dephosphorylation. Upregulates the expression of protein phosphatase 1 (PP1), which dephosphorylates the key ‘Ser-418’ residue of FOXP3, thereby inactivating FOXP3 and rendering Treg cells functionally defective . Key mediator of cell death in the anticancer action of BCG-stimulated neutrophils in combination with DIABLO/SMAC mimetic in the RT4v6 bladder cancer cell line . / The TNF intracellular domain (ICD) form induces IL12 production in dendritic cells.

Involvement in disease

Psoriatic arthritis (PSORAS) [MIM:607507]: An inflammatory, seronegative arthritis associated with psoriasis. It is a heterogeneous disorder ranging from a mild, non-destructive disease to a severe, progressive, erosive arthropathy. Five types of psoriatic arthritis have been defined: asymmetrical oligoarthritis characterized by primary involvement of the small joints of the fingers or toes; asymmetrical arthritis which involves the joints of the extremities; symmetrical polyarthritis characterized by a rheumatoid like pattern that can involve hands, wrists, ankles, and feet; arthritis mutilans, which is a rare but deforming and destructive condition; arthritis of the sacroiliac joints and spine (psoriatic spondylitis). . Note: Disease susceptibility is associated with variations affecting the gene represented in this entry.

Protein sequence and domain

Belongs to the tumor necrosis factor family.

Protein post-translational modifications

The soluble form derives from the membrane form by proteolytic processing. The membrane-bound form is further proteolytically processed by SPPL2A or SPPL2B through regulated intramembrane proteolysis producing TNF intracellular domains (ICD1 and ICD2) released in the cytosol and TNF C-domain 1 and C-domain 2 secreted into the extracellular space. / The membrane form, but not the soluble form, is phosphorylated on serine residues. Dephosphorylation of the membrane form occurs by binding to soluble TNFRSF1A/TNFR1. / O-glycosylated; glycans contain galactose, N-acetylgalactosamine and N-acetylneuraminic acid.

Protein cellular localization

Cell membrane / Single-pass type II membrane protein / Membrane; Single-pass type II membrane protein / Secreted / Secreted / Secreted

Research area

All research areas>Growth Factors and Hormones>Tumor Necrosis Factor
(View all antibody categories related to Growth Factors and Hormones)

Note

AntibodyPlus can customize TNF-alpha Antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provide antibody trial sample for your own antibody validation and collects antibody reviews.

Supplier

St John’s Laboratory Ltd.

Product type

Primary antibody

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Immunofluorescence analysis of Human stomach tissue

1: TNF-alpha Polyclonal Antibody(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunofluorescence analysis of Human stomach tissue

1: TNF-alpha Polyclonal Antibody(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.


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