Rabbit Polyclonal USP28 antibody (A10400)

$99.00$259.00

Reactivity: Human
Applications: WB
Conjugation: Unconjugated
Supplier: ABclonal Inc.

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Rabbit Polyclonal USP28 antibody (A10400)

Supplier: ABclonal Inc.

Recommended applications: WB

Recommended dilution:

WB 1:500 – 1:2000

Recommended protocols: check protocols

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Alternative names:

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Deubiquitinating enzyme 28 antibody|KIAA1515 antibody|Ubiquitin carboxyl terminal hydrolase 28 antibody|Ubiquitin carboxyl-terminal hydrolase 28 antibody|ubiquitin carboxyl-terminal hydrolase 28 variant 1 antibody|Ubiquitin specific peptidase 28 antibody|Ubiquitin specific processing protease 28 antibody|Ubiquitin specific protease 28 antibody|Ubiquitin thioesterase 28 antibody|Ubiquitin thiolesterase 28 antibody|Ubiquitin-specific-processing protease 28 antibody|UBP28_HUMAN antibody|USP 28 antibody|USP28 antibody|USP28 protein antibody|Anti-USP28 antibody (ab188240)

SCBT cat No:

Name

Rabbit Polyclonal USP28 antibody

Catalogue No.

A10400

Reactivity

Human

Immunogen

Recombinant protein of human USP28

Host

Rabbit

Applications

WB

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

118kDa

Formulation

USP28 antibody was tube-contained.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Purification

Affinity purification

Storage

Store at -20 Celsius degree. Avoid freeze / thaw cycles.

Protein function

Deubiquitinase involved in DNA damage response checkpoint and MYC proto-oncogene stability. Involved in DNA damage induced apoptosis by specifically deubiquitinating proteins of the DNA damage pathway such as CLSPN. Also involved in G2 DNA damage checkpoint, by deubiquitinating CLSPN, and preventing its degradation by the anaphase promoting complex/cyclosome (APC/C). In contrast, it does not deubiquitinate PLK1. Specifically deubiquitinates MYC in the nucleoplasm, leading to prevent MYC degradation by the proteasome: acts by specifically interacting with isoform 1 of FBXW7 (FBW7alpha) in the nucleoplasm and counteracting ubiquitination of MYC by the SCF(FBW7) complex. In contrast, it does not interact with isoform 4 of FBXW7 (FBW7gamma) in the nucleolus, allowing MYC degradation and explaining the selective MYC degradation in the nucleolus. Deubiquitinates ZNF304, hence preventing ZNF304 degradation by the proteasome and leading to the activated KRAS-mediated promoter hypermethylation and transcriptional silencing of tumor suppressor genes (TSGs) in a subset of colorectal cancers (CRC) cells (PubMed:24623306).

Protein sequence and domain

Belongs to the peptidase C19 family. USP28 subfamily.; Contains 1 UIM (ubiquitin-interacting motif) domain.; Contains 1 USP domain.

Protein post-translational modifications

Degradaded upon nickel ion level or hypoxia exposure.; Phosphorylated upon DNA damage at Ser-67 and Ser-714, by ATM or ATR (PubMed:16901786). Phosphorylated by PRKD1 (PubMed:24623306).

Protein cellular localization

Nucleus, nucleoplasm .

Background

The ubiquitin-dependent protein degradation pathway is essential for proteolysis of intracellular proteins and peptides. Enzymes that remove ubiquitin from ubiquitin-conjugated peptides, like USP28, affect the fate and degradation of intracellular proteins and are essential for maintenance of cell-free ubiquitin pools (Valero et al., 2001).

Note

AntibodyPlus can customize USP28 antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provides antibody trial sample for your own antibody validation.

Product type

Primary antibody

Supplier

ABclonal Inc.

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