Rabbit Polyclonal VIM antibody (STJ96243)

$99.00$319.00

Reactivity: Human, Mouse, Rat
Applications: WB, IHC
Conjugation: Unconjugated
Supplier: St John’s Laboratory Ltd.

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Rabbit Polyclonal VIM antibody (STJ96243)

Supplier: St John’s Laboratory Ltd.

Recommended applications: WB, IHC, IF, ELISA

Recommended dilution: WB 1:500-1:2000; IHC 1:100-1:300; IF 1:200-1:1000; ELISA 1:10000;

Recommended protocols: check protocols

Image descriptions:

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Alternative names:

Check alternative names for the antibody

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VIM antibody,|CTRCT30 antibody|Epididymis luminal protein 113 antibody|FLJ36605 antibody|HEL113 antibody|VIM antibody|VIME_HUMAN antibody|Vimentin antibody|Anti-Vimentin antibody [V9] – Cytoskeleton Marker (ab8069)
SCBT cat No: sc-16673|sc-12973|sc-16674|sc-248328|sc-248326|sc-166656|sc-390188|sc-398474|sc-12934|sc-377077|sc-48403|sc-48340|sc-25381|sc-9690|sc-7739|sc-7737|sc-66913|

Name

VIM Polyclonal Antibody

Catalogue No.

STJ96243

Reactivity

Human, Mouse, Rat

Specificity

VIM Polyclonal Antibody detects endogenous levels of VIM protein.

Immunogen

Synthesized peptide derived from VIM at AA range 380-460

Host

Rabbit

Applications

WB, IHC, IF, ELISA

Recommended dilution

WB 1:500-1:2000; IHC 1:100-1:300; IF 1:200-1:1000; ELISA 1:10000;

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

53 kDa

Formulation

VIM Antibody was tube-contained. Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Concentration

1 mg/ml

Purification

VIM Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage

-20 Celsius degree. Avoid repeated freeze/thaw cycles.

Alternative antibody names

Vimentin antibody

Database links

Human UniProt/Swiss-Prot:P08670;Mouse UniPort/Swiss-Prot: P20152;Rat UniProt/Swiss-Port: P31000;Human Entrez Gene: 7431;Mouse Entrez Gene: 22352;Rat Entrez Gene: Rn.2710

Protein names

Vimentin

Protein function

Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally. / Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2.

Protein tissue specificity

Highly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt’s lymphoma cell lines. Expressed in many hormone-independent mammary carcinoma cell lines.

Involvement in disease

Cataract 30 (CTRCT30) [MIM:116300]: An opacification of the crystalline lens of the eye that frequently results in visual impairment or blindness. Opacities vary in morphology, are often confined to a portion of the lens, and may be static or progressive. In general, the more posteriorly located and dense an opacity, the greater the impact on visual function. . Note: The disease is caused by mutations affecting the gene represented in this entry.

Protein sequence and domain

The central alpha-helical coiled-coil rod region mediates elementary homodimerization. / The [IL]-x-C-x-x-[DE] motif is a proposed target motif for cysteine S-nitrosylation mediated by the iNOS-S100A8/A9 transnitrosylase complex. / Belongs to the intermediate filament family.

Protein post-translational modifications

Filament disassembly during mitosis is promoted by phosphorylation at Ser-55 as well as by nestin (By similarity). One of the most prominent phosphoproteins in various cells of mesenchymal origin. Phosphorylation is enhanced during cell division, at which time vimentin filaments are significantly reorganized. Phosphorylation by PKN1 inhibits the formation of filaments. Phosphorylated at Ser-56 by CDK5 during neutrophil secretion in the cytoplasm. Phosphorylated by STK33. / O-glycosylated during cytokinesis at sites identical or close to phosphorylation sites, this interferes with the phosphorylation status. / S-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex.

Protein cellular localization

Cytoplasm

Research area

All research areas>Lymphocyte Signaling>CD15
(View all antibody categories related to Lymphocyte Signaling)

Note

AntibodyPlus can customize VIM Antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provide antibody trial sample for your own antibody validation and collects antibody reviews.

Supplier

St John’s Laboratory Ltd.

Product type

Primary antibody

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Immunohistochemical analysis of paraffin embedded Human stomach cancer tissue

1:VIM Polyclonal Antibody was diluted at 1:200(4 Celcius degree,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval(>98 Celcius degree,20min).
3:Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Human liver cancer tissue

1:VIM Polyclonal Antibody was diluted at 1:200(4 Celcius degree,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval(>98 Celcius degree,20min).
3:Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Rat KIDNEY tissue

1:VIM Polyclonal Antibody was diluted at 1:200(4 Celcius degree,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval(>98 Celcius degree,20min).
3:Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Mouse spleen tissue

1:VIM Polyclonal Antibody was diluted at 1:200(4 Celcius degree,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval(>98 Celcius degree,20min).
3:Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.


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