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Supplier : Arigo Biolaboratories
|Production type||ELISA Kits|
|Specificity||No significant cross-reactivity or interference with Human TNF- alpha,TNF- beta;porcine TNF- alpha|
|Target Name||TNF alpha|
|Conjugation Note||Substrate: TMB and read at 450 nm|
|Sample Type||Serum, Plasma, Cell culture supernatants|
|Sample Volume||100 ul|
|Full Name||tumor necrosis factor|
|Alternate Names||Check alternative names for the Rat TNF alpha ELISA Kit Expand
APC1 Elisa kits|APC1 protein Elisa kits|Cachectin Elisa kits|DIF Elisa kits|Differentiation inducing factor Elisa kits|Macrophage cytotoxic factor Elisa kits|Tnf Elisa kits|TNF superfamily, member 2 Elisa kits|TNF, macrophage derived Elisa kits|TNF, monocyte derived Elisa kits|TNF-a Elisa kits|TNF-alpha Elisa kits|TNFA Elisa kits|TNFA_HUMAN Elisa kits|TNFSF2 Elisa kits|Tumor necrosis factor alpha Elisa kits|Tumor necrosis factor Elisa kits|Tumor necrosis factor ligand superfamily member 2 Elisa kits|Tumor Necrosis Factor, Membrane Form Elisa kits|Tumor necrosis factor, soluble form Elisa kits
|Assay Time||4 hours|
|Storage instruction||Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components or simply ask Antibodyplus for ELISA troublesooting.|
|Note||Antibodyplus and Arigo provide the most validated ELISA kits for researchers. For laboratory research only, not for drug, diagnostic or other use.|
|User’s manual of Rat TNF alpha ELISA Kit||Download|
|Cat No.|| |
|Conjugation note|| |
Substrate: TMB and read at 450 nm
|Sample type|| |
Serum, Plasma, Cell culture supernatants
|Sample Volume|| |
|Target name|| |
|Assay time|| |
|Store at|| |
4 Celcius degree
|Storage instruction|| |
Store the kit at 2-8 Celcius degree. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Tumor Necrosis Factor Alpha (TNF alpha) is a protein secreted by lipopolysaccharide stimulated macrophages, and causes tumor necrosis when injected into tumor bearing mice. TNF alpha is believed to mediate pathogenic shock and tissue injury associated with endotoxemia. TNF alpha exists as a multimer of two, three, or five noncovalently linked units, but shows a single 17 kDa band following SDS PAGE under non reducing conditions. TNF alpha is closely related to the 25 kDa protein Tumor Necrosis Factor beta (lymphotoxin), sharing the same receptors and cellular actions. TNF alpha causes cytolysis or cytostasis of certain transformed cells, being synergistic with interferon gamma in its cytotoxicity. Although it has little effect on many cultured normal human cells, TNF alpha appears to be directly toxic to vascular endothelial cells. Other actions of TNF alpha include stimulating growth of human fibroblasts and other cell lines, activating polymorphonuclear neutrophils and osteoclasts, and induction of interleukin 1, prostaglandin E2 and collagenase production. TNF alpha is currently being evaluated in treatment of certain cancers and AIDS Related Complex.
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