Goat Anti-Rabbit IgG Antibody (H&L) [HRP], pAb

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Overview

Abbreviated name-1 Goat Anti Rabbit IgG (H&L) [HRP]; Anti Rabbit IgG (H&L) [HRP]
Description GenScript Goat Anti-Rabbit IgG (H&L) [HRP] Polyclonal Antibody is highly purified from goat antiserum by immunoaffinity chromatography and then conjugated to horseradish peroxidase. It reacts with rabbit IgG heavy and light chains.
Immunogen Full-length rabbit IgG (purified)
Host Species Goat
Conjugation Horseradish peroxidase (HRP)
Purification Method Immunoaffinity chromatography

 

Properties

Formulation 1 mg/ml, lyophilized with PBS, pH 7.4, containing 1% BSA and 0.01% thimerosal
Reconstitution Reconstitute the lyophilized antibody with deionized water (or equivalent) to a final concentration of 1.0 mg/ml.
Ig Subclass Goat IgG
Specificity This antibody specifically recognizes rabbit IgG and other species are not tested.
Storage The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

 

Applications

GenScript Goat Anti-Rabbit IgG (H&L) [HRP] is suitable for immunoblotting (western and dot blots), ELISA, immunoperoxidase electron microscopy, immunohistochemistry, and other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
The investigator must determine the ideal working concentration for each specific application. The ideal working concentration must take into account such factors as secondary antibody affinity, antigen concentration, sensitivity of the detection method, temperature, and the length of the incubations. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 1:10,000-1:50,000
Western blot: 1:1,000-1:20,000
Other applications: user-optimized
Technical Manual ELISA Protocol(pdf)
Western Blot Protocol(pdf)
FACS Protocol(pdf)
IHC Protocol(pdf)
IP Protocol(pdf)
Size

1mg

Product type

Secondary antibody

Reviews


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Displaying 1 - 10 of 118

Antibody customer reviews & validation data - 208

Validation data ID: 208

Submission date: 2017-07-17

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Result figure:
Result figure

Figure description:

A clear band of Her2 has been detected.

Product name

Rabbit Polyclonal Neu Antibody

Product catalog No.

STJ94416

Product type

Primary antibody

Supplier

St John's Laboratory

Application type

Western Blot(WB)

Antibody specificity rating

Good

Antibody overall rating

Good

Your comments

N/A

Detailed protocol

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Antibody customer reviews & validation data - 206

Validation data ID: 206

Submission date: 2017-06-30

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Result figure:
Result figure

Figure description:

Figure is labelled with sample names very well

Product name

Rabbit polyclonal GAS6 antibody (A8545)

Product catalog No.

A8545

Product type

Primary antibody

Supplier

ABclonal

Application type

Western Blot(WB)

Antibody specificity rating

Good

Antibody overall rating

Good

Your comments

This lot of Gas6 antibody works well on my WB, hopefully, you have enough antibody for our research from the same lot.

Detailed protocol

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Antibody customer reviews & validation data - 205

Validation data ID: 205

Submission date: 2017-06-30

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Result figure:
Result figure

Figure description:

A is 4x, B is 10x (scale=100 um, white arrows point cilia cells, blue triangles point peripheral nerves) of whole-mount immunofluorescence on stage-31 embryo. The cc of the antibody was 1:1000 and blocking solution was 10% normal goat serum + 2% BSA in PBS-Tx100 0.1%

Product name

Anti-Beta II Tubulin

Product catalog No.

STJ97122

Product type

Primary antibody

Supplier

St John's Laboratory

Application type

Immunofluorescence (IF)

Antibody specificity rating

Excellent

Antibody overall rating

Excellent

Your comments

This antibody worked well.

Detailed protocol

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Antibody customer reviews & validation data - 204

Validation data ID: 204

Submission date: 2017-06-30

View Details

Result figure:
  • Result figure
  • Result figure

Figure description:

C2C12 mouse muscle cells were transfected with a plasmid expressing connexin 43 IRES FusionRed under the control of a mature muscle promoter MCK. Normally C2C12 cells do not express Connexin 43 in mature myotubes. They do express connexin 43 in immature myoblasts. The negative control are the same cells with the FusionRed tag only. There is still some staining of connexin 43, but not in the mature myotubes.

Product name

Anti-Connexin 43 antibody

Product catalog No.

STJ92411

Product type

Primary antibody

Supplier

St John's Laboratory

Application type

Immunocytochemistry (ICC)

Antibody specificity rating

Excellent

Antibody overall rating

Excellent

Your comments

This antibody worked very well for this assay.

Detailed protocol

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Antibody customer reviews & validation data - 203

Validation data ID: 203

Submission date: 2017-06-30

View Details

Result figure:
  • Result figure
  • Result figure

Figure description:

The antibody was tested for functionality in cell immunofluorescence. The sample was C2C12 mouse muscle cells transfected with a plasmid that expresses connexin 40 IRES FusionRed under a mature muscle promoter MCK. The negative control were the same cells with just the FusionRed tag. It appears that there is light non-specific binding.

Product name

Anti-Connexin 40 antibody

Product catalog No.

STJ96742

Product type

Primary antibody

Supplier

St John's Laboratory

Application type

Immunocytochemistry (ICC)

Antibody specificity rating

Not work in this case

Antibody overall rating

Not work in this case

Your comments

The antibody had some non-specific binding in the negative control. The experimental did not show clear dots which is what is expected for connexin binding.

Detailed protocol

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Antibody customer reviews & validation data - 202

Validation data ID: 202

Submission date: 2017-06-30

View Details

Result figure:
  • Result figure
  • Result figure

Figure description:

C2C12 mouse muscle cells were transfected with a plasmid expressing connexin 37 IRES FusionRed using a mature muscle promoter MCK. The negative control is the same cells but with only the FusionRed tag. The connexin 37 antibody non-specifically bound to the cells.

Product name

Anti-Connexin 37 antibody

Product catalog No.

STJ92408

Product type

Primary antibody

Supplier

St John's Laboratory

Application type

Immunocytochemistry (ICC)

Antibody specificity rating

Not work in this case

Antibody overall rating

Not work in this case

Your comments

This antibody did not work in cell immunofluorescence. There was a lot of non-specific binding in the negative control.

Detailed protocol

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Antibody customer reviews & validation data - 201

Validation data ID: 201

Submission date: 2017-06-29

View Details

Result figure:
p-Foxo3a.pdf

Figure description:

Human multiple myeloma cell line KMS11 was cultured in RPMI-8226 media and then lysates are made in RIPA buffer supplemented with phosphatase and proteinase inhibitors. 2ug of protein was loaded in two wells and electrophoresed for 30min at 200V BIORAD electrophoresis unit. Gel was transferred to PVDF membrane and blot evaluated for the expression of p-FOXO3a.

Product name

Rabbit Polyclonal Phospho-FoxO3A (S253) antibody

Product catalog No.

STJ90275

Product type

Primary antibody

Supplier

St John's Laboratory

Application type

Western Blot(WB)

Antibody specificity rating

Good

Antibody overall rating

Good

Your comments

This antibody worked well for me. I had tried antibodies from other companies also but only this worked.

Detailed protocol

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Antibody customer reviews & validation data - 196

Validation data ID: 196

Submission date: 2017-05-26

View Details


Figure description:

The figure show a protein of 94KDa,
The expure time using X-Ray film was for 30 seconds

Product name

c-Myc Tag Antibody, mAb, Mouse

Product catalog No.

A00704-100

Product type

Primary antibody

Supplier

GenScript

Application type

Western Blot(WB)

Antibody specificity rating

Excellent

Antibody overall rating

Excellent

Your comments

It worked great

Detailed protocol

View Details

Antibody customer reviews & validation data - 195

Validation data ID: 195

Submission date: 2017-05-26

View Details


Figure description:

The figure shows a protein of 80KDa.
The acquisition was performed using an X-Ray film, the specific band is clearly visible but it would be excellent without the non spefific band.

Product name

Rabbit Polyclonal FLAG-tag antibody

Product catalog No.

STJ90108

Product type

Primary antibody

Supplier

St John's Laboratory

Application type

Western Blot(WB)

Antibody specificity rating

Fair

Antibody overall rating

Good

Your comments

I used a dilution of 1:1000, incubating the membrane with the antibody overnight at 4 degrees.
I detected along with my specific protein band, also one unspecific which maybe adjusting the condtions I could get rid of it.

Detailed protocol

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Antibody customer reviews & validation data - 194

Validation data ID: 194

Submission date: 2017-05-25

View Details


Figure description:

is provided in the Figure.

Product name

GABAA delta antibody

Product catalog No.

STJ93192

Product type

Primary antibody

Supplier

St John's Laboratory

Application type

Western Blot(WB)

Antibody specificity rating

Good

Antibody overall rating

Excellent

Your comments

single band

Detailed protocol

View Details

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