THETM beta Actin Antibody, mAb, Mouse [2D1D10] (A00702-100)

$69.00$229.00

Reactivity: mouse, rabbit, chicken, human, hamster, cow, goat, fish, pig
Applications: ELISA,WB,
Conjugation: Various
Supplier: GenScript Inc.

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THETM beta Actin Antibody, mAb, Mouse [2D1D10] (A00702-100)

Supplier: GenScript Inc.

Recommended applications:

ELISA: 0.1-1.0 ug/ml
Western blot: 0.1-1.0 ug/ml
Other applications: user-optimized

Recommended protocols: check protocols

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THETM beta Actin Antibody, mAb, Mouse

Name

THETM beta Actin Antibody, mAb, Mouse

Catalogue No.

A00702-100

Reactivity

mouse, rabbit, chicken, human, hamster, cow, goat, fish, pig

Specificity

THETM beta Actin Antibody, mAb, Mouse reacts with mouse, rabbit, chicken, human, hamster, cow, goat, fish, and pig. It has not yet been tested in other species.

Immunogen

A synthetic peptide DDDIAALVVDNGSG coupled to KLH

Host

Mouse

Applications

ELISA, WB

Fusion partner

Spleen cells are fused with SP2/0-Ag14 mouse myeloma cells

Clonality

Monoclonal

Conjugation

Unconjugated

Isotype

IgG2a, k

Formulation

40 ug, lyophilized with PBS, pH 7.4, containing 1% BSA and 0.02% sodium azide. Note: 100 ug, 200ug (GenScript, A00702), lyophilized with PBS, pH 7.4, containing 0.02% sodium azide, without BSA

Reconstitution

Reconstitute the lyophilized antibody with deionized water to the final concentration of 0.5 mg/ml.

Purification

Protein A or G affinity column

Storage

The antibody is stable in lyophilized form if stored at -20 Celsius degree or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8 Celsius degree. For long term storage, aliquot and store at -20 Celsius degree or below. Avoid repeated freezing and thawing cycles.

Citation

Gruenwald K.,et al. Sc65 is a novel endoplasmic reticulum protein that regulates bone mass homeostasisJ Bone Miner Res.2013Aug; Au KY.,et al. Engineering a switch-on peptide to ricin A chain for increasing its specificity towards HIV-infected cells.Biochim Biophys Acta.2013Nov; Palavicini JP.,et al. RanBP9 Overexpression Down-Regulates Phospho-Cofilin, Causes Early Synaptic Deficits and Impaired Learning, and Accelerates Accumulation of Amyloid Plaques in the Mouse Brain.J Alzheimers Dis.2013Nov; Wang H.,et al. COPS5 (Jab1) Protein Increases Site Processing of Amyloid Precursor Protein and Amyloid Peptide Generation by Stabilizing RanBP9 Protein Levels.J Biol Chem.2013Sep;288(37):26668-77 Palavicini JP.,et al. RanBP9 aggravates synaptic damage in the mouse brain and is inversely correlated to spinophilin levels in Alzheimer¡®s brain synaptosomes.Cell Death Dis.2013Jun;4:e667 Shen Y.,et al. Efficient Protection and Transfection of Small Interfering RNA by Cationic Shell-Crosslinked Knedel-Like Nanoparticles.Nucleic Acid Ther.2013Apr;23(2):95-108 Hayes CD.,et al. Striking reduction of amyloid plaque burden in an Alzheimer’s mouse model after chronic administration of carmustine.BMC Med.2013Mar;11:81 Micale V.,et al. Cholecystokinin in White Sea Bream: Molecular Cloning, Regional Expression, and Immunohistochemical Localization in the Gut after Feeding and Fasting.PLoS One.2012Dec;7(12):e52428 Hayes CD.,et al. Chronic Cladribine Administration Increases Amyloid Beta Peptide Generation and Plaque Burden in Mice.PLoS One.2012Otc;7(10):e45841 Ke H.,et al. CYLD Inhibits Melanoma Growth and Progression through Suppression of the JNK/AP-1 and 1-Integrin Signaling Pathways.J Invest Dermatol.2013Jan;133(1):221-9 H Wang, et al. RanBP9 Overexpression Accelerates Loss of Pre and Postsynaptic Proteins in the APdeltaE9 Transgenic Mouse Brain. Plos One. 2014Jan.;9(1):e85484

Note

AntibodyPlus can customize THETM beta Actin Antibody, mAb, Mouse antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com

Product type

Primary antibody

Supplier

Genscript Inc.

Reviews


There are no reviews yet.

Only logged in customers who have purchased this product may leave a review

Displaying 1 - 10 of 118

Antibody customer reviews & validation data - 208

Validation data ID: 208

Submission date: 2017-07-17

View Details

Result figure:
Result figure

Figure description:

A clear band of Her2 has been detected.

Product name

Rabbit Polyclonal Neu Antibody

Product catalog No.

STJ94416

Product type

Primary antibody

Supplier

St John's Laboratory

Application type

Western Blot(WB)

Antibody specificity rating

Good

Antibody overall rating

Good

Your comments

N/A

Detailed protocol

View Details

Antibody customer reviews & validation data - 206

Validation data ID: 206

Submission date: 2017-06-30

View Details

Result figure:
Result figure

Figure description:

Figure is labelled with sample names very well

Product name

Rabbit polyclonal GAS6 antibody (A8545)

Product catalog No.

A8545

Product type

Primary antibody

Supplier

ABclonal

Application type

Western Blot(WB)

Antibody specificity rating

Good

Antibody overall rating

Good

Your comments

This lot of Gas6 antibody works well on my WB, hopefully, you have enough antibody for our research from the same lot.

Detailed protocol

View Details

Antibody customer reviews & validation data - 205

Validation data ID: 205

Submission date: 2017-06-30

View Details

Result figure:
Result figure

Figure description:

A is 4x, B is 10x (scale=100 um, white arrows point cilia cells, blue triangles point peripheral nerves) of whole-mount immunofluorescence on stage-31 embryo. The cc of the antibody was 1:1000 and blocking solution was 10% normal goat serum + 2% BSA in PBS-Tx100 0.1%

Product name

Anti-Beta II Tubulin

Product catalog No.

STJ97122

Product type

Primary antibody

Supplier

St John's Laboratory

Application type

Immunofluorescence (IF)

Antibody specificity rating

Excellent

Antibody overall rating

Excellent

Your comments

This antibody worked well.

Detailed protocol

View Details

Antibody customer reviews & validation data - 204

Validation data ID: 204

Submission date: 2017-06-30

View Details

Result figure:
  • Result figure
  • Result figure

Figure description:

C2C12 mouse muscle cells were transfected with a plasmid expressing connexin 43 IRES FusionRed under the control of a mature muscle promoter MCK. Normally C2C12 cells do not express Connexin 43 in mature myotubes. They do express connexin 43 in immature myoblasts. The negative control are the same cells with the FusionRed tag only. There is still some staining of connexin 43, but not in the mature myotubes.

Product name

Anti-Connexin 43 antibody

Product catalog No.

STJ92411

Product type

Primary antibody

Supplier

St John's Laboratory

Application type

Immunocytochemistry (ICC)

Antibody specificity rating

Excellent

Antibody overall rating

Excellent

Your comments

This antibody worked very well for this assay.

Detailed protocol

View Details

Antibody customer reviews & validation data - 203

Validation data ID: 203

Submission date: 2017-06-30

View Details

Result figure:
  • Result figure
  • Result figure

Figure description:

The antibody was tested for functionality in cell immunofluorescence. The sample was C2C12 mouse muscle cells transfected with a plasmid that expresses connexin 40 IRES FusionRed under a mature muscle promoter MCK. The negative control were the same cells with just the FusionRed tag. It appears that there is light non-specific binding.

Product name

Anti-Connexin 40 antibody

Product catalog No.

STJ96742

Product type

Primary antibody

Supplier

St John's Laboratory

Application type

Immunocytochemistry (ICC)

Antibody specificity rating

Not work in this case

Antibody overall rating

Not work in this case

Your comments

The antibody had some non-specific binding in the negative control. The experimental did not show clear dots which is what is expected for connexin binding.

Detailed protocol

View Details

Antibody customer reviews & validation data - 202

Validation data ID: 202

Submission date: 2017-06-30

View Details

Result figure:
  • Result figure
  • Result figure

Figure description:

C2C12 mouse muscle cells were transfected with a plasmid expressing connexin 37 IRES FusionRed using a mature muscle promoter MCK. The negative control is the same cells but with only the FusionRed tag. The connexin 37 antibody non-specifically bound to the cells.

Product name

Anti-Connexin 37 antibody

Product catalog No.

STJ92408

Product type

Primary antibody

Supplier

St John's Laboratory

Application type

Immunocytochemistry (ICC)

Antibody specificity rating

Not work in this case

Antibody overall rating

Not work in this case

Your comments

This antibody did not work in cell immunofluorescence. There was a lot of non-specific binding in the negative control.

Detailed protocol

View Details

Antibody customer reviews & validation data - 201

Validation data ID: 201

Submission date: 2017-06-29

View Details

Result figure:
p-Foxo3a.pdf

Figure description:

Human multiple myeloma cell line KMS11 was cultured in RPMI-8226 media and then lysates are made in RIPA buffer supplemented with phosphatase and proteinase inhibitors. 2ug of protein was loaded in two wells and electrophoresed for 30min at 200V BIORAD electrophoresis unit. Gel was transferred to PVDF membrane and blot evaluated for the expression of p-FOXO3a.

Product name

Rabbit Polyclonal Phospho-FoxO3A (S253) antibody

Product catalog No.

STJ90275

Product type

Primary antibody

Supplier

St John's Laboratory

Application type

Western Blot(WB)

Antibody specificity rating

Good

Antibody overall rating

Good

Your comments

This antibody worked well for me. I had tried antibodies from other companies also but only this worked.

Detailed protocol

View Details

Antibody customer reviews & validation data - 196

Validation data ID: 196

Submission date: 2017-05-26

View Details


Figure description:

The figure show a protein of 94KDa,
The expure time using X-Ray film was for 30 seconds

Product name

c-Myc Tag Antibody, mAb, Mouse

Product catalog No.

A00704-100

Product type

Primary antibody

Supplier

GenScript

Application type

Western Blot(WB)

Antibody specificity rating

Excellent

Antibody overall rating

Excellent

Your comments

It worked great

Detailed protocol

View Details

Antibody customer reviews & validation data - 195

Validation data ID: 195

Submission date: 2017-05-26

View Details


Figure description:

The figure shows a protein of 80KDa.
The acquisition was performed using an X-Ray film, the specific band is clearly visible but it would be excellent without the non spefific band.

Product name

Rabbit Polyclonal FLAG-tag antibody

Product catalog No.

STJ90108

Product type

Primary antibody

Supplier

St John's Laboratory

Application type

Western Blot(WB)

Antibody specificity rating

Fair

Antibody overall rating

Good

Your comments

I used a dilution of 1:1000, incubating the membrane with the antibody overnight at 4 degrees.
I detected along with my specific protein band, also one unspecific which maybe adjusting the condtions I could get rid of it.

Detailed protocol

View Details

Antibody customer reviews & validation data - 194

Validation data ID: 194

Submission date: 2017-05-25

View Details


Figure description:

is provided in the Figure.

Product name

GABAA delta antibody

Product catalog No.

STJ93192

Product type

Primary antibody

Supplier

St John's Laboratory

Application type

Western Blot(WB)

Antibody specificity rating

Good

Antibody overall rating

Excellent

Your comments

single band

Detailed protocol

View Details

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