Vitamin B12 ELISA Kit (ARG80816)

$799.00

Size: 96 wells
Conjugation: HRP
Supplier: Arigo Biolaboratories

Overview


Supplier : Arigo Biolaboratories

 Production type ELISA Kits
Tested Reactivity Other
Tested Application ELISA
Specificity
Target Name Vitamin B12
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm
Sensitivity 0.3 ng/ml
Detection Range
Sample Type food
Sample Volume 50 ul
Precision

CV: <10%

Full Name Vitamin B12
Alternate Names Check alternative names for the Vitamin B12 ELISA Kit

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Application Instructions

 

Assay Time 1 h (RT/shaker), 20 min (RT/dark)


Properties

 

Form 96 wells
Storage instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components or simply ask Antibodyplus for ELISA troublesooting.
Note Antibodyplus and Arigo provide the most validated ELISA kits for researchers. For laboratory research only, not for drug, diagnostic or other use.
User’s manual of Vitamin B12 ELISA Kit  Download
size

96 wells

Cat No.

ARG80816

Conjugation note

Substrate: TMB and read at 450 nm

Sensitivity

0.3 ng/ml

Sample type

food

Sample Volume

50 ul

Precision

CV: <10%

Target name

Vitamin B12

Assay time

1 h (RT/shaker), 20 min (RT/dark)

Store at

4 Celcius degree

Storage instruction

Store the kit at 2-8 Celcius degree. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.

Background

Vitamin B12 as a trace element belongs to the biologi?cally important chelate formers. The basic unit con?sists of a corrin ring with cobalt as a central atom. Cobalt is sixfold coordinated by four nitrogen atoms, one cyanide and a dimethylbenzimidazol group. Vitamin B12 forms a stable complex, which is absorbed in the lower part of the small intestine, with the so-called intrinsic factor present in the gastric juice. A lack of vitamin B12 can lead among other things to pernicious anemia. This disease is not generated by an insufficient supply of vitamin B12, but by the ab?sence of intrinsic factor. A pernicious anemia can be treated by a high dosage of vitamin B12. The existing detection procedures are mainly microbiological methods, but also HPLC and thin-layer chromatography, all of which are associated with a high amount of time and instrumentation. With the present test kit it is possible, to determine vitamin B12 quantitatively in vitaminated food in a significantly faster way (2.5 to 4 hours inclusive sam?ple pretreatment) compared with a conventional microbiological assay (24 to 48 hours).

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