Vitamin H (Biotin) ELISA Kit (ARG80817)

$799.00

Size: 96 wells
Conjugation: HRP
Supplier: Arigo Biolaboratories

Overview


Supplier : Arigo Biolaboratories

 Production type ELISA Kits
Tested Reactivity Other
Tested Application ELISA
Specificity
Target Name Vitamin H
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm
Sensitivity 0.5 ng/ml
Detection Range
Sample Type food
Sample Volume 50 ul
Precision

CV: <10%

Full Name Vitamin H / Biotin
Alternate Names Check alternative names for the Vitamin H (Biotin) ELISA Kit

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Application Instructions

 

Assay Time 1 h (RT/shaker), 20 min (RT/dark)


Properties

 

Form 96 wells
Storage instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components or simply ask Antibodyplus for ELISA troublesooting.
Note Antibodyplus and Arigo provide the most validated ELISA kits for researchers. For laboratory research only, not for drug, diagnostic or other use.
User’s manual of Vitamin H (Biotin) ELISA Kit  Download
size

96 wells

Cat No.

ARG80817

Conjugation note

Substrate: TMB and read at 450 nm

Sensitivity

0.5 ng/ml

Sample type

food

Sample Volume

50 ul

Precision

CV: <10%

Target name

Vitamin H

Assay time

1 h (RT/shaker), 20 min (RT/dark)

Store at

4 Celcius degree

Storage instruction

Store the kit at 2-8 Celcius degree. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.

Background

Biotin serves as the prosthetic group of enzymes, which catalyze carboxylations in the organism. For this purpose, biotin is bound via its carboxy group to lysin residues of carboxylases, and the transfer of carbon dioxide takes place after its attachment to a nitrogen atom of biotin, forming the so-called active carbon dioxide. The awareness of the population for a good health and its interest in healthy nutrition has increased sig?nificantly during the last years. After the content of vitamins in his nourishment has gained importance for the consumer, food has partially been vitaminized by the manufacturer. When there exists a lack of biotin, seborrhoea, dermatitis, anorexia, muscle pain, tiredness and nervous disorders can appear. As biotin is synthesized by the human intestinal flora, deficiency symptoms are rare, appear however after excessive ingestion of raw egg white, which can be explained by its content of biotin-binding avidin.

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